Life Sciences Commons^™

Examining Transcriptional Regulators During Muscle Development In Drosophila Melanogaster, Chaamy Yapa

Student Theses and Dissertations

In Drosophila melanogaster embryos, a distinct approach to study the transcriptional regulation is to examine the larval somatic muscle development. Transcription factors are essential regulatory proteins that help to control gene expression and respond to signaling pathways and various cues. Today, there are at least twenty transcription factors that have been discovered to contribute to the development of the 30 distinct larval somatic muscles in each abdominal hemisegment of Drosophila melanogaster. Several studies have already been conducted on muscle regulatory transcription factors including midline and apterous. These transcription factors were shown to control the development of muscles through mutant …

Validating A New In Vivo Model To Study Als, Izabela J. Cimachowska

Student Theses and Dissertations

Buildup of oxidative stress and mitochondrial dysfunction are well known characteristics of both sporadic and hereditary amyotrophic lateral sclerosis (ALS). While both forms of the disease seem to arise from common cellular dysfunction, the genetic disease is studied to a much greater extent. Engineering novel animal models of the sporadic form of the disease is crucial for development of druggable targets to treat ALS and understand the underlying mechanisms. Interestingly, accumulation of oxidative stress by exacerbated emission of reactive oxygen species (ROS) from presynaptic mitochondria is a hallmark of both hereditary and sporadic ALS. Previous work by our laboratory showed …

The Effects Of Glycolytic Mutations In Drosophila Melanogaster Muscle Development, Coco Lim

Student Theses and Dissertations

Muscle atrophy, or muscle wasting, is caused due to lack of physical activity for an extended period of time, due to muscle diseases (such as muscle dystrophies), cancer chemotherapies, and aging. It is also extensively found on astronauts after spaceflight, particularly missions of long durations. Muscle cells are dependent on different metabolic pathways to optimize Adenosine triphosphate (ATP) production to compensate for muscle exertion. Glycolysis converts glucose into ATP producing pyruvate, which can be sent into the citric acid cycle or converted to lactate (lactic acid). Muscles preferentially use lactate production, despite the fact that fewer molecules of ATP are …

The Significance Of Sonic Branding To Strategically Stimulate Consumer Behavior: Content Analysis Of Four Interviews From Jeanna Isham’S “Sound In Marketing” Podcast, Ina Beilina

Student Theses and Dissertations

Purpose:
Sonic branding is not just about composing jingles like McDonald’s “I’m Lovin’ It.” Sonic branding is an industry that strategically designs a cohesive auditory component of a brand’s corporate identity. This paper examines the psychological impact of music and sound on consumer behavior reviewing studies from the past 40 years and investigates the significance of stimulating auditory perception by infusing sound in consumer experience in the modern 2020s.

Design/methodology/approach:
Qualitative content analysis of audio media was used to test two hypotheses. Four archival oral interview recordings from Jeanna Isham’s podcast “Sound in Marketing” featuring the sonic branding experts …

Mitochondrial Distribution Of Glycine Receptors In Motor Neuron Cell Lines, Katsiaryna Milashevich

Student Theses and Dissertations

Although non-essential, glycine plays an important role in major metabolic reactions and is most known for its anti-inflammatory effects. An accumulation of contemporary research has shown that glycine is able to stabilize membrane potential using glycine receptors at the cellular level and to protect mitochondrial function directly, whether it is from inflammation, heavy metal poisoning, or ischemia-induced neuroinflammation. In this research, the existence of a hypothetical mitochondrial glycine receptor is examined. Immunofluorescence imaging was used to examine the presence of the glycine receptor subunits alpha 1 and alpha 2 in both non- differentiated and differentiated neuroblastoma cell lines. The preliminary …

Motor Neuron Connections And Innervation Of Muscles In Drosophila Melanogaster, Sharon Tang

Student Theses and Dissertations

Drosophila melanogaster have two sets of muscle systems: larval muscles and adult muscles. The larval somatic muscle system is established during embryogenesis and is necessary for hatching, feeding, and crawling of the larvae. Like humans, Drosophila muscles have individual characteristics, like unique sizes, shapes, orientations, attachment sites, and innervation patterns by motor neurons. These properties are encoded by a group of transcriptional regulators that are expressed in specific muscle subsets. Currently, there are over twenty known transcription factors required for the development of the 30 distinct larval somatic muscles and specification of those muscle properties. We have examined how muscle-specific …

The Distribution, Abundance, And Gut Microbiome Of Ribbed Mussel, Geukensia Demissa, Across Natural And Restored Salt Marshes In Jamaica Bay, New York, Bethany Freynk

Student Theses and Dissertations

Coastal wetlands perform valuable functions by protecting shorelines from floodwaters and storm surges, providing habitats for marine species, and improving local water quality. Unfortunately, over half of the area of global wetlands has been lost over the past century. Locally, in Jamaica Bay (Queens, NY), loss of wetlands has exceeded 98%. Restoration of Jamaica Bay marshes began in 2003. Ribbed mussels, Geukensia demissa, live symbiotically with marsh plants and have been shown to stabilize sediments and provide organic nutrients that enhance marsh plant growth. Mussels are suspension feeders, which collect algae from seawater and deposit organic matter in marsh sediments. …

Examining The Roles Of Genetic And Environmental Factors In Drosophila Melanogaster Hematopoiesis And Innate Immune System, Minkyung Lee

Student Theses and Dissertations

Nearly 24 million people are affected by autoimmune diseases in the United States. Main causes of autoimmune diseases have been attributed to genetic predisposition and environmental exposure to chemicals such as hormones and pesticides. Due to the large population that are affected by autoimmune diseases, it is critical to understand the mechanisms behind them. In this study, we sought to explore both genetic and environmental factors that affect hematopoiesis, or the formation of specific blood cells, and immune system in Drosophila melanogaster. As Drosophila melanogaster have conserved pathways of hematopoiesis as humans, they were used as the model organism …

Genetic Basis Of Larval Crystal Cell Quantity Variation In The Drosophila Genetic Reference Panel (Dgrp), Brian Tang

Student Theses and Dissertations

Crystal cells are one of three requisite hemocytes that take part in fighting infection and wound healing in Drosophila melanogaster (common fruit flies). The developmental genetics of crystal cell formation is only beginning to be discovered. To address this question, we performed a Genome-Wide Association Study (GWAS) on larval crystal cell number from 78 isolines of the Drosophila Genetic Reference Panel (DGRP) collection. The DGRP consists of naturally caught fruit flies that are inbred to near homozygosity with completely sequenced genomes. By placing the wandering third instar larvae under heatshock, a process that induces the melanization of crystal cells, …

The Effect Of Protein Import On Membrane Potential, Ruth Hartke

Student Theses and Dissertations

Mitochondria are essential organelles often referred to as the “power plants” of eukaryotic cells. Energy conversion in mitochondria relies on oxidation of metabolite intermediates by protein components of the electron transport chain and establishment of an electrochemical gradient across the inner membrane. Some of the proteins involved are encoded by mitochondrial DNA, but the vast majority, over 98%, has to be imported from the cytosol. Protein import complexes form pores in the outer and inner membranes to mediate the process. However, opening of these pores would in theory jeopardize formation of the electrochemical gradient. In this study we examined this …

Study Of Alternative Functions Of The Mitochondrial Protein Bak, Ma Su Su Aung

Student Theses and Dissertations

Research in the past 15 years has established roles for the pro-apoptotic proteins Bax and Bak in release of death signaling molecules from mitochondria. The process involves relocation of cytoplasmic Bax into the mitochondrial outer membrane to form a giant pore, MAC. Another MAC component, Bak, is constitutively present in the outer membrane regardless of apoptotic stimulation. In this study we investigated the role of Bak in mitochondrial function outside the context of apoptosis. We examined the effects of Bak elimination on emission of reactive oxygen species from mitochondria. Our results indicate a disturbance of free-radical production both in cultured …