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- ALDH1A1; Cancer stem cells; CD44; Curcumin; Esophageal cancer; NF-κB; Tumorsphere (1)
- Battery-operated microcentrifuge; DNA extraction; Manually-operated centrifuge (1)
- Bolivia; Extinction; Psittacidae; PVA; Savanna (1)
- Cancer stem cell; Curcumin; Lewis lung carcinoma; Phototoxicity (1)
- Cyanobacteria (1)
Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Construction And Transformation Of A Thermotoga-E. Coli Shuttle Vector, Zhaohui Xu, Dongmei Han, Stephen M. Norris
Construction And Transformation Of A Thermotoga-E. Coli Shuttle Vector, Zhaohui Xu, Dongmei Han, Stephen M. Norris
Biological Sciences Faculty Publications
Background: Thermotoga spp. are attractive candidates for producing biohydrogen, green chemicals, and thermostable enzymes. They may also serve as model systems for understanding life sustainability under hyperthermophilic conditions. A lack of genetic tools has hampered the investigation and application of these organisms. This study aims to develop a genetic transfer system for Thermotoga spp. Results: Methods for preparing and handling Thermotoga solid cultures under aerobic conditions were optimized. A plating efficiency of similar to 50% was achieved when the bacterial cells were embedded in 0.3% Gelrite. A Thermotoga-E. coli shuttle vector pDH10 was constructed using pRQ7, a cryptic mini-plasmid found …
Properties Of Lewis Lung Carcinoma Cells Surviving Curcumin Toxicity, Michael Eric Geusz, Dejun Yan
Properties Of Lewis Lung Carcinoma Cells Surviving Curcumin Toxicity, Michael Eric Geusz, Dejun Yan
Biological Sciences Faculty Publications
The anti-inflammatory agent curcumin can selectively eliminate malignant rather than normal cells. The present study examined the effects of curcumin on the Lewis lung carcinoma (LLC) cell line and characterized a subpopulation surviving curcumin treatments. Cell density was measured after curcumin was applied at concentrations between 10 and 60 μM for 30 hours. Because of the high cell loss at 60 μM, this dose was chosen to select for surviving cells that were then used to establish a new cell line. The resulting line had approximately 20% slower growth than the original LLC cell line and based on ELISA contained …
Extraction Of Dna From Plant And Fungus Tissues In Situ, Scott O. Rogers, Amal S. Abu Almakarem, Katie L. Heilman, Heather L. Conger, Yury M. Shtarkman
Extraction Of Dna From Plant And Fungus Tissues In Situ, Scott O. Rogers, Amal S. Abu Almakarem, Katie L. Heilman, Heather L. Conger, Yury M. Shtarkman
Biological Sciences Faculty Publications
Background: When samples are collected in the field and transported to the lab, degradation of the nucleic acids contained in the samples is frequently observed. Immediate extraction and precipitation of the nucleic acids reduces degradation to a minimum, thus preserving accurate sequence information. An extraction method to obtain high quality DNA in field studies is described. Findings. DNA extracted immediately after sampling was compared to DNA extracted after allowing the sampled tissues to air dry at 21°C for 48 or 72 hours. While DNA extracted from fresh tissues exhibited little degradation, DNA extracted from all tissues exposed to 21°C air …
Unraveling The Determinants Of Protrusion Formation, Mita Varghese, Peter Gorsevski, Marilyn L. Cayer, Nancy S. Boudreau, Carol A. Heckman
Unraveling The Determinants Of Protrusion Formation, Mita Varghese, Peter Gorsevski, Marilyn L. Cayer, Nancy S. Boudreau, Carol A. Heckman
Biological Sciences Faculty Publications
A computerized morphometric classification technique based on latent factors reveals major protrusion classes: factors 4, 5, and 7. Previous work showed that factor 4 represented filopodia, 5 the distribution of lamellar cytoplasm, and 7 a blunt protrusion. We explore the relationship of focal contact (FC) characteristics and their integrated actin cables to factors values. The results show that FC maturation/cytoskeletal integration affects factor 5, because FC elongation/integration was correlated with its values. On the contrary, 7 values decreased with maturation, so cable or FC size or their integration must be restricted to form these protrusions. Where integration did occur, the …
Population Viability Analysis Of The Blue-Throated Macaw (Ara Glaucogularis) Using Individual-Based And Cohort-Based Pva Programs, Juan Luis Bouzat, Rosa I. Strem
Population Viability Analysis Of The Blue-Throated Macaw (Ara Glaucogularis) Using Individual-Based And Cohort-Based Pva Programs, Juan Luis Bouzat, Rosa I. Strem
Biological Sciences Faculty Publications
We developed a demographic model to perform a population viability analysis (PVA) of the Blue-throated Macaw (Ara glaucogularis), a critically endangered species endemic to Bolivia. PVA simulations were run using individual based (VORTEX 9.72) and cohort-based (RAMAS GIS 4.0) programs. A baseline simulation allowed for the assessment of the status of the species based on estimates of extinction risk and population declines under current conditions of abundance and habitat availability. The role of multiple demographic, environmental, and anthropogenic parameters was evaluated to assess changes affecting population declines and extinction risk. The baseline simulation showed that the Blue-throated Macaw has a …
Effects Of Curcumin On Stem-Like Cells In Human Esophageal Squamous Carcinoma Cell Lines, Michael Eric Geusz, Taghreed N. Almanaa, Roudabeh J. Jamasbi
Effects Of Curcumin On Stem-Like Cells In Human Esophageal Squamous Carcinoma Cell Lines, Michael Eric Geusz, Taghreed N. Almanaa, Roudabeh J. Jamasbi
Biological Sciences Faculty Publications
Background: Many cancers contain cell subpopulations that display characteristics of stem cells. Because these cancer stem cells (CSCs) appear to provide resistance to chemo-radiation therapy, development of therapeutic agents that target CSCs is essential. Curcumin is a phytochemical agent that is currently used in clinical trials to test its effectiveness against cancer. However, the effect of curcumin on CSCs is not well established. The current study evaluated curcumin-induced cell death in six cancer cell lines derived from human esophageal squamous cell carcinomas. Moreover, these cell lines and the ones established from cells that survived curcumin treatments were characterized.Methods: Cell loss …
Plasticity Of Total And Intracellular Phosphorus Quotas In Microcystis Aeruginosa Cultures And Lake Erie Algal Assemblages, Robert Michael Mckay, Matthew A. Saxton, Robert J. Arnold, Richard A. Bourbonniere, Steven W. Wilhelm
Plasticity Of Total And Intracellular Phosphorus Quotas In Microcystis Aeruginosa Cultures And Lake Erie Algal Assemblages, Robert Michael Mckay, Matthew A. Saxton, Robert J. Arnold, Richard A. Bourbonniere, Steven W. Wilhelm
Biological Sciences Faculty Publications
Blooms of the potentially toxic cyanobacterium Microcystis are common events globally, and as a result significant resources continue to be dedicated to monitoring and controlling these events. Recent studies have shown that a significant proportion of total cell-associated phosphorus (P) in marine phytoplankton can be surface adsorbed; as a result studies completed to date do not accurately report the P demands of these organisms. In this study we measure the total cell-associated and intracellular P as well as growth rates of two toxic strains of Microcystis aeruginosa Kütz grown under a range of P concentrations. The results show that the …
An Evaluation Of The Role Of Sensory Drive In The Evolution Of Lake Malawi Cichlid Fishes, Adam R. Smith, Moira J. Van Staaden, Karen L. Carleton
An Evaluation Of The Role Of Sensory Drive In The Evolution Of Lake Malawi Cichlid Fishes, Adam R. Smith, Moira J. Van Staaden, Karen L. Carleton
Biological Sciences Faculty Publications
Although the cichlids of Lake Malawi are an important model system for the study of sensory evolution and sexual selection, the evolutionary processes linking these two phenomena remain unclear. Prior works have proposed that evolutionary divergence is driven by sensory drive, particularly as it applies to the visual system.While evidence suggests that sensory drive has played a role in the speciation of Lake Victoria cichlids, the findings fromseveral lines of research on cichlids of LakeMalawi are not consistent with the primary tenets of this hypothesis. More specifically, three observations make the sensory drive model implausible in Malawi: (i) a lack …