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Development Of Quantitative Sensitive Immunochromatographic Assays For Detection Of Aflatoxin B1, Liu Jing, Wang Shuying, Liu Daofeng, Lai Weihua
Development Of Quantitative Sensitive Immunochromatographic Assays For Detection Of Aflatoxin B1, Liu Jing, Wang Shuying, Liu Daofeng, Lai Weihua
Food and Machinery
The aim of this work was to establish a highly sensitive quantitative colloidal gold immunochromatographic assay with a test strip reader for the detection of aflatoxin B1 using colloidal gold as label. When the labeling pH was 6.0, the concentration of antigen on T-line was 1.5 mg/mL, the concentration of antibody labeled on the colloidal gold was 4 μg/mL, and the dosage of gold-labeled antibody was 1.2 μL, the sensitivity of the assay was 5.7 ng/L. The results of accelerated aging experiment indicated that the strips shelf time was more than a year. It was proved that this method …