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The Cardiac L-Type Calcium Channel Distal Carboxyl- Terminus Auto-Inhibition Is Regulated By Calcium, Shawn M. Crump
The Cardiac L-Type Calcium Channel Distal Carboxyl- Terminus Auto-Inhibition Is Regulated By Calcium, Shawn M. Crump
Theses and Dissertations--Physiology
The L-type calcium channel (LTCC) provides trigger Ca2+ for sarcoplasmic reticulum Ca2+-release and LTCC function is influenced by interacting proteins including the LTCC Distal Carboxyl-terminus (DCT) and calmodulin. DCT is proteolytically cleaved, and re-associates with the LTCC complex to regulate calcium channel function. DCT reduces LTCC barium current (IBa,L) in reconstituted channel complexes, yet the contribution of DCT to ICa,L in cardiomyocyte systems is unexplored. This study tests the hypothesis that DCT attenuates cardiomyocyte ICa,L. We measured LTCC current and Ca2+ transients with DCT co-expressed in murine cardiomyocytes. We also heterologously co-expressed DCT and CaV1.2 constructs …