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Full-Text Articles in Life Sciences

The Pursuit Of Non-Corn-Based Biofuel Production As A Means Of Countering Rising Crop Costs, Rachele M. Hendricks Jun 2015

The Pursuit Of Non-Corn-Based Biofuel Production As A Means Of Countering Rising Crop Costs, Rachele M. Hendricks

Rachele M Hendricks-Sturrup

The “Renewable Fuel Standard” was the first renewable fuel mandate set forth by the United States Environmental Protection Agency initially under the Energy Policy Act (EPAct) of 2005. Alternative fuels are defined by the US EPA as alternatives to conventional fuels such as gasoline and diesel. Alternative fuels are lauded for their “clean energy” attributes and also because they can be resourced domestically, allowing for per-capita reductions in greenhouse gas emissions with less dependency on foreign energy resources. Alternative fuel conversions are methods used to convert gasoline and/or diesel engines into engines that operate on natural gas, alcohols, or electricity, …


High-Throughput Protein Purification For X-Ray Crystallography And Nmr, Rachele M. Hendricks-Sturrup Feb 2009

High-Throughput Protein Purification For X-Ray Crystallography And Nmr, Rachele M. Hendricks-Sturrup

Rachele M Hendricks-Sturrup

In structural biology, the most critical issue is the availability of high-quality samples. “Structural-biology-grade” proteins must be generated in a quantity and quality suitable for structure determination using X-ray crystallography or nuclear magnetic resonance. The additional challenge for structural genomics is the need for high numbers of proteins at low cost where protein targets quite often have low sequence similarities, unknown properties and are poorly characterized. The purification procedures must reproducibly yield homogeneous proteins or their derivatives containing marker atom(s) in milligram quantities. The choice of protein purification and handling procedures plays a critical role in obtaining high-quality protein samples. …