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Articles 1 - 30 of 32
Full-Text Articles in Life Sciences
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Telomerase Activity Is Required For The Telomere G-Overhang Structure In Trypanosoma Brucei, Ranjodh Sandhu, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes fatal human African trypanosomiasis and evades the host immune response by regularly switching its major surface antigen, VSG, which is expressed exclusively from subtelomeric loci. Telomere length and telomere proteins play important roles in regulating VSG silencing and switching. T. brucei telomerase plays a key role in maintaining telomere length, and T. brucei telomeres terminate in a single-stranded 3′ G-rich overhang. Understanding the detailed structure of the telomere G-overhang and its maintenance will contribute greatly to better understanding telomere maintenance mechanisms. Using an optimized adaptor ligation assay, we found that most T. brucei telomere G-overhangs end in …
The Future Perspective: Metabolomics In Laboratory Medicine For Inborn Errors Of Metabolism, Yana Sandlers
The Future Perspective: Metabolomics In Laboratory Medicine For Inborn Errors Of Metabolism, Yana Sandlers
Chemistry Faculty Publications
Metabolomics can be described as a simultaneous and comprehensive analysis of small molecules in a biological sample. Recent technological and bioinformatics advances have facilitated large-scale metabolomic studies in many areas, including inborn errors of metabolism (IEMs). Despite significant improvements in the diagnosis and treatment of some IEMs, it is still challenging to understand how genetic variation affects disease progression and susceptibility. In addition, a search for new more personalized therapies and a growing demand for tools to monitor the long-term metabolic effects of existing therapies set the stage for metabolomics integration in preclinical and clinical studies. While targeted metabolomics approach …
Hybrid Extended Kalman Filtering And Noise Statistics Optimization For Produce Wash State Estimation, Vahid Azimi, Daniel Munther, Seyed Abolfazl Fakoorian, Thang Tien Nguyen, Daniel J. Simon
Hybrid Extended Kalman Filtering And Noise Statistics Optimization For Produce Wash State Estimation, Vahid Azimi, Daniel Munther, Seyed Abolfazl Fakoorian, Thang Tien Nguyen, Daniel J. Simon
Mathematics and Statistics Faculty Publications
Food-borne diseases associated with fresh produce consistently cause serious public health issues. Although sanitization measures are utilized to enhance the safety of fresh produce, strategies that neglect the dynamic nature of commercial wash processes are limited, creating the potential for pathogen cross-contamination and major disease outbreaks. In light of this risk, there is an urgent need for new control approaches during produce washing to reduce the probability of outbreaks. As an important step in this direction, a hybrid extended Kalman filter (HEKF) and particle swarm optimization (PSO)-based noise statistics optimization are designed for a produce wash system. The HEKF uses …
Development And Validation Of A Novel Lc–Ms/Ms Method For Simultaneous Determination Of Abiraterone And Its Seven Steroidal Metabolites In Human Serum: Innovation In Separation Of Diastereoisomers Without Use Of A Chiral Column, Mohammad Alyamani, Zhenfei Li, Sunil K. Upadhyay, David J. Anderson, Richard J. Auchus, Nima Sharifi
Development And Validation Of A Novel Lc–Ms/Ms Method For Simultaneous Determination Of Abiraterone And Its Seven Steroidal Metabolites In Human Serum: Innovation In Separation Of Diastereoisomers Without Use Of A Chiral Column, Mohammad Alyamani, Zhenfei Li, Sunil K. Upadhyay, David J. Anderson, Richard J. Auchus, Nima Sharifi
Chemistry Faculty Publications
Abiraterone acetate (AA), the prodrug of abiraterone, is FDA-approved for the treatment of castration-resistant prostate cancer. Abiraterone is metabolized in patients to a more potent analogue, D4A. However, we have recently reported that this analogue is further metabolized to additional metabolites in patients treated with AA. Here, we present a liquid chromatography-tandem mass spectrometry method developed to resolve and detect abiraterone and its seven metabolites in human serum using an AB Sciex Qtrap 5500 mass analyzer coupled with a Shimadzu Nexera UPLC station. Analytes and the internal standard (abiraterone-d4) were extracted from human serum using the liquid–liquid extraction procedure. The …
A Comparative Analysis Of Translesion Dna Synthesis Catalyzed By A High-Fidelity Dna Polymerase, Anvesh Dasari, Tejal Deodhar, Anthony J. Berdis
A Comparative Analysis Of Translesion Dna Synthesis Catalyzed By A High-Fidelity Dna Polymerase, Anvesh Dasari, Tejal Deodhar, Anthony J. Berdis
Chemistry Faculty Publications
Translesion DNA synthesis (TLS) is the ability of DNA polymerases to incorporate nucleotides opposite and beyond damaged DNA. TLS activity is an important risk factor for the initiation and progression of genetic diseases such as cancer. In this study, we evaluate the ability of a high-fidelity DNA polymerase to perform TLS with 8-oxo-guanine (8-oxo-G), a highly pro-mutagenic DNA lesion formed by reactive oxygen species. Results of kinetic studies monitoring the incorporation of modified nucleotide analogs demonstrate that the binding affinity of the incoming dNTP is controlled by the overall hydrophobicity of the nucleobase. However, the rate constant for the …
Circling The Drain: Regulating Nutrient Pollution From Agricultural Sources, William Gutermuth
Circling The Drain: Regulating Nutrient Pollution From Agricultural Sources, William Gutermuth
Journal of Law and Health
The 2014 Toledo, Ohio tap water ban has, at least temporarily, put a spotlight on United States water supplies. Consequently, many Americans have begun to take a closer look at the quality of the fresh water bodies being used to supply tap water to their homes. Therefore, this Note analyzes the problems currently threatening the lakes, rivers, and other surface waters that are the source of fresh drinking water for huge populations in the United States. Part II examines the problem of nutrient pollution and explains the harmful effects it has on human health. Part III provides an overview of …
Weird Science: Frankenstein Foods And States As Laboratories Of Democracy, Jennifer Mcgee
Weird Science: Frankenstein Foods And States As Laboratories Of Democracy, Jennifer Mcgee
Journal of Law and Health
The National Bioengineered Food Disclosure Standard (the 'National Standard') was signed into law July 29, 2016. This Article analyzes the National Standard and posits that Vermont’s Act 120 was a more effective labeling law because it safeguarded consumer sovereignty. The State regulatory scheme in place prior to the passage of the National Standard satisfied consumer demand for disclosure while allowing for necessary experimentation with GMO labeling. Part I provides an overview of the current federal scheme regulating GMOs. Part II analyzes of the conflict surrounding GMOs and labeling. Given that analysis, Part III compares the disclosure requirement of the National …
Inhibiting Translesion Dna Synthesis As An Approach To Combat Drug Resistance To Dna Damaging Agents, Jung-Suk Choi, Seol Kim, Edward Motea, Anthony J. Berdis
Inhibiting Translesion Dna Synthesis As An Approach To Combat Drug Resistance To Dna Damaging Agents, Jung-Suk Choi, Seol Kim, Edward Motea, Anthony J. Berdis
Chemistry Faculty Publications
Anti-cancer agents exert therapeutic effects by damaging DNA. Unfortunately, DNA polymerases can effectively replicate the formed DNA lesions to cause drug resistance and create more aggressive cancers. To understand this process at the cellular level, we developed an artificial nucleoside that visualizes the replication of damaged DNA to identify cells that acquire drug resistance through this mechanism. Visualization is achieved using "click" chemistry to covalently attach azide-containing fluorophores to the ethynyl group present on the nucleoside analog after its incorporation opposite damaged DNA. Flow cytometry and microscopy techniques demonstrate that the extent of nucleotide incorporation into genomic DNA is enhanced …
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Trypanosoma Brucei Rap1 Maintains Telomere And Subtelomere Integrity By Suppressing Terra And Telomeric Rna: Dna Hybrids, Vishal Nanavaty, Ranjodh Sandhu, Sanaa E. Jehi, Unnati M. Pandya, Bibo Li
Biological, Geological, and Environmental Faculty Publications
Trypanosoma brucei causes human African trypanosomiasis and regularly switches its major surface antigen, VSG, thereby evading the host's immune response. VSGs are monoallelically expressed from subtelomeric expression sites (ESs), and VSG switching exploits subtelomere plasticity. However, subtelomere integrity is essential for T. brucei viability. The telomeric transcript, TERRA, was detected in T. brucei previously. We now show that the active ES-adjacent telomere is transcribed. We find that TbRAP1, a telomere protein essential for VSG silencing, suppresses VSG gene conversionmediated switching. Importantly, TbRAP1 depletion increases the TERRA level, which appears to result from longer read-through into the telomere downstream of the …
Organ Donation: A Comparison Of Altruistic And Market-Based Systems, Cameron Caputi
Organ Donation: A Comparison Of Altruistic And Market-Based Systems, Cameron Caputi
The Downtown Review
One of the most heavily regulated aspects of the Health Care industry is the organ donation system (“Legislation and Policy”). Regulations in this area ensure the quality of the organs and morality of the process through which they were procured. This system, however, is failing in at least one sense; the number of patients requiring organ transplants is increasing, but the number of donors remains stagnant (Gordon, Patel, Sohn, Hippen, Sherman , 2014). Due to the lack of available transplant organs, a debate has been sparked on whether the United States government should allow for the purchasing of these organs.
Unreplicable: The Unscientific Nature Of Science Journals, Ernest M. Oleksy
Unreplicable: The Unscientific Nature Of Science Journals, Ernest M. Oleksy
The Downtown Review
Academia shapes the way our species looks at veracity and defines what is deemed as well-founded science. The platform for researchers to make their work known is academic journals. The prerogative of these journals is to disseminate technically sound work so that the public may be informed of up-to-date advances in scientific fields. However, these journals are products on the market whose ultimate purpose is to garner a following that will make the producers money. This results in research that does not have statistically significant findings, or replications of past experiments which are integral to supporting the findings of the …
Hmba Is A Putative Hsp70 Activator Stimulating Hexim1 Expression That Is Down-Regulated By Estrogen, Rati Lama, Chunfang Gan, Nethrie Idippily, Viharika Bobba, David Danielpour, Monica Montano, Bin Su Ph.D.
Hmba Is A Putative Hsp70 Activator Stimulating Hexim1 Expression That Is Down-Regulated By Estrogen, Rati Lama, Chunfang Gan, Nethrie Idippily, Viharika Bobba, David Danielpour, Monica Montano, Bin Su Ph.D.
Chemistry Faculty Publications
Hexamethylene bis-acetamide inducible protein 1 (HEXIM1) is identified as a novel inhibitor of estrogen stimulated breast cell growth, and it suppresses estrogen receptor-a transcriptional activity. HEXIM1 protein level has been found to be downregulated by estrogens. Recently, HEXIM1 has been found to inhibit androgen receptor transcriptional activity as well. Researchers have used Hexamethylene bisacetamide (HMBA) for decades to stimulate HEXIM1 expression, which also inhibit estrogen stimulated breast cancer cell gene activation and androgen stimulated prostate cancer gene activation. However, the direct molecular targets of HMBA that modulate the induction of HEXIM1 expression in mammalian cells have not been identified. Based …
African Clawed Toads Response To The Distance Choice Of Lateral Line Stimuli, Casey Iyasere
African Clawed Toads Response To The Distance Choice Of Lateral Line Stimuli, Casey Iyasere
Undergraduate Research Posters 2017
African Clawed Toads are a model organism for research on sensory integration due to their lateral line system that allows them to sense water movement. This study further investigates the choices made by toads when presented with two lateral line stimuli in the form of surface waves. When such stimuli are initiated simultaneously, toads tend to turn towards the more rostral stimulus or the nearer stimulus, waves from which arrive first. We introduced a delay between stimuli to allow the waves from the farther stimulus to arrive first. Toads were more likely to respond to a single stimulus than they …
The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack
The Effect Of Site Characteristics On The Reproductive Output Of Lesser Celandine (Ranunculus Ficaria), Justin P. Kermack
ETD Archive
Ranunculus ficaria L., an ephemeral perennial invasive plant brought over from Europe, is becoming widespread throughout the Northeastern United States. This herbaceous buttercup is able to create extensive dense mats that limit native species growth. Taking advantage of an early growing season and rapid reproduction rates, this species can create dense monocultures, which threatens native communities and ecosystems. Elimination of native spring ephemerals results in decreased biodiversity. A better understanding of how R. ficaria responds to site characteristics is needed to prioritize management efforts toward high-risk sites.
Ranunculus ficaria abundance and reproductive output (seed, bulbil and tuber production rates) were …
Determination Of Novel Metabolites Of Therapeutic Agents Used In The Treatment Of Castration-Resistant Prostate Cancer, Mohammad Alyamani
Determination Of Novel Metabolites Of Therapeutic Agents Used In The Treatment Of Castration-Resistant Prostate Cancer, Mohammad Alyamani
ETD Archive
Despite an array of improved treatment options over the past decade, prostate cancer remains the second leading cause of cancer mortality for men in the United States. Abiraterone and galeterone are oral steroidal compounds that are used to treat metastatic castration-resistant prostate cancer (CRPC). Abiraterone blocks 17a-hydroxylase/17,20-lyase (CYP17A1), an enzyme required for androgen synthesis. Galeteron inhibits CYP17A1, blocks the androgen receptor (AR), and decreases AR protein levels. Both drugs share the same structure with endogenous androgens such as dehydroepiandrosterone, which are substrates for the enzyme, 3ß-hydroxysteroid dehydrogenase (3ßHSD). Metabolites of 3ßHSD undergo further metabolism to produce the AR ligand, testosterone …
Cryptochromes Regulate Igf-1 Production And Signaling Through Control Of Jak2-Dependent Stat5b Phosphorylation, Amol Santosh Charudhari
Cryptochromes Regulate Igf-1 Production And Signaling Through Control Of Jak2-Dependent Stat5b Phosphorylation, Amol Santosh Charudhari
ETD Archive
Insulin-like growth factor (IGF) signaling plays an important role in cell growth and proliferation and is implicated in regulation of cancer, metabolism, and aging. Here we report that IGF-1 level in blood and IGF-1 signaling demonstrates circadian rhythms. Circadian control occurs through cryptochromes (CRYs)—transcriptional repressors and components of the circadian clock. IGF-1 rhythms are disrupted in Cry-deficient mice, and IGF-1 level is reduced by 80% in these mice, which leads to reduced IGF signaling. In agreement, Cry-deficient mice have reduced body (~30% reduction) and organ size. Down-regulation of IGF-1 upon Cry deficiency correlates with reduced Igf-1 mRNA expression in the …
Restoration Of Black Oak (Quercus Velutina) Sand Barrens Via Three Different Habitat Management Approaches, David J. Kriska
Restoration Of Black Oak (Quercus Velutina) Sand Barrens Via Three Different Habitat Management Approaches, David J. Kriska
ETD Archive
Disturbance regimes, i.e. frequent fires, historically maintained oak barrens until European settlement patterns, and eventually, Smoky the Bear and the fire suppression campaign of the U.S. Forest Service snuffed out the periodic flames. In the absence of a disturbance regime, ground layer floral composition at many historical oak sand barrens will change predominantly because of a buildup of leaf litter and shading of the soils. Termed mesophication, this process of ecological succession will drive Black Oak Sand Barrens to an alternate steady state. A survey conducted on Singer Lake Bog in Green, Ohio, demonstrated that succession shifted the community to …
Age And Sex-Specific Effect Of Caloric Restriction On Circadian Clock And Longevity-Associated Gene Expression, Arten Andreyevich Astafev
Age And Sex-Specific Effect Of Caloric Restriction On Circadian Clock And Longevity-Associated Gene Expression, Arten Andreyevich Astafev
ETD Archive
The rhythms in the expression of circadian clock genes are affected by calorie restriction (CR), a dietary paradigm known to increase lifespan. In our current study, we show that circadian rhythms are influenced by sex and the effects of CR are different between males and females. In particular, we found a group of clock genes which showed a sex-dependent difference in expression, as well as in response to CR (Rev-Erb α, Ror γ and both Cryptochromes: Cry1 and Cry2 genes). Two clock genes showed no difference in expression but their response to CR showed sexual dimorphism (Ror α and Rev-Erb …
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Consequences Of Dam Removal On Mussel Assemblages (Unionidae) In The Cuyahoga River, Nicholas P. Hogya, Rachel E. Andrikanich, Ryan J. Trimbath, Robert A. Krebs
Biological, Geological, and Environmental Faculty Publications
The primary objective of a new survey of the Cuyahoga River was to assess species richness and population abundance of various mussel species in the family Unionidae throughout the Middle Cuyahoga River and upper parts of the Lower Cuyahoga River. Historically, few records existed for this river between Lake Rockwell and the Cuyahoga Valley National Park, a region in which four dams have been removed in the past 12 years. Timed visual surveys were conducted during low flow conditions and by using tactile techniques in deeper water. Throughout the survey, only 37 live animals were located across the 18 Middle …
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Developmental Stages Identified In The Trophozoite Of The Free-Living Alveolate Flagellate Colpodella Sp. (Apicomplexa), Raghavendra Yadavalli, Tobili Y. Sam-Yellowe
Biological, Geological, and Environmental Faculty Publications
In this study we performed light, immunofluorescent and transmission electron microscopy of Colpodella trophozoites to characterize trophozoite morphology and protein distribution. The use of Giemsa staining and antibodies to distinguish Colpodella life cycle stages has not been performed previously. Rhoptry and β-tubulin antibodies were used in immunofluorescent assays (IFA) to identify protein localization and distribution in the trophozoite stage of Colpodella (ATCC 50594). We report novel data identifying “doughnut-shaped” vesicles in the cytoplasm and apical end of Colpodella trophozoites reactive with antibodies specific to Plasmodium merozoite rhoptry proteins. Giemsa staining and immunofluorescent microscopy identified different developmental stages of Colpodella trophozoites, …
Developmental Manganese Neurotoxicity In Rats: Cognitive Deficits In Allocentric And Egocentric Learning And Memory, Nina Atanasova, Robyn M. Amos-Kroohs, Laurie L. Davenport, Zuhair I. Abdulla, Matthew R. Skelton, Charles V. Vorhees, Michael T. Williams
Developmental Manganese Neurotoxicity In Rats: Cognitive Deficits In Allocentric And Egocentric Learning And Memory, Nina Atanasova, Robyn M. Amos-Kroohs, Laurie L. Davenport, Zuhair I. Abdulla, Matthew R. Skelton, Charles V. Vorhees, Michael T. Williams
Philosophy and Religious Studies Department Faculty Publications
No abstract provided.
Introducing Delays Between Two Lateral Line Stimuli Alters Choices By African Clawed Toads, Kevin Goth, Austin Schaffer
Introducing Delays Between Two Lateral Line Stimuli Alters Choices By African Clawed Toads, Kevin Goth, Austin Schaffer
Undergraduate Research Posters 2017
African clawed toads (Xenopus laevis) use their lateral line system to detect prey. The African clawed frog can determine the distance of the origin of a surface wave. This allows the frog to differentiate which stimulus is more important for prey capture. The African clawed toads were put into a glass basin which was filled with water. Above the water basin are four rods that are controlled through a computer program which allow the rods to touch the surface of the water. After recording, each frame was examined in the videos to determine the turn angle and stimuli distance of …
An Evaluation Of Unionidae Diversity In The Rocky River And How Population Density Has Changed Over Time In Comparison To Observations Made Over 15 Years Ago, Jamil Wilson
Undergraduate Research Posters 2017
This study was undertaken to determine the changes in freshwater bivalve population since the survey conducted in 2001 in the northern region of the Rocky River, Ohio, USA. During our study, we discovered 69 live specimens and 58 shells most of which were found in two distinct locations near the top and bottom of the area surveyed. Our finds consisted of eight species in comparison to the nine that were seen in the same area of the 2001 study. Our results showed that Leptodea fragilis populations have decreased in the main stream of the Rocky River since the observation 2001, …
Express Recombinant Tb12990 Protein From E. Coli For Dna Binding Analysis, Alex Lotozynski
Express Recombinant Tb12990 Protein From E. Coli For Dna Binding Analysis, Alex Lotozynski
Undergraduate Research Posters 2017
Trypanosoma brucei is a parasitic protozoan species, causing sleeping sickness in humans and nagana in livestock. Its vector, the tsetse fly, feeds on an infected host and passes the parasites into the bloodstream of other mammalian hosts. The unique challenge for elimination is its complex process of antigenic variation, where the parasite regularly switches its major surface antigen, VSG. When the parasite enters the mammalian host, the host responds by making a corresponding antibody against its major surface antigen, VSG. However, although most parasites are eliminated, a small population can escape due to their altered VSG coat. The expression sites …
Morphological Study And Biochemical Characterization Of The Alveolate Flagellate Colpodella Sp. (Apicomplexa) In A Diprotist Culture With Bodo Caudatus, Lauren Dulik, Raghavendra Yadavalli
Morphological Study And Biochemical Characterization Of The Alveolate Flagellate Colpodella Sp. (Apicomplexa) In A Diprotist Culture With Bodo Caudatus, Lauren Dulik, Raghavendra Yadavalli
Undergraduate Research Posters 2017
Plasmodium falciparum, the causative agent of severe human malaria shares the presence of apical complex organelles with the free-living predatory alveolate, Colpodella sp. In this study we investigated morphological, biochemical and molecular characteristics of Colpodella sp. in a diprotist culture containing Bodo caudatus as prey. Colpodella attaches to its prey using the apical end. Attachment lasted for approximately 20 minutes while the cytoplasmic contents of the prey were aspirated into the posterior food vacuole of Colpodella in a process known as myzocytosis. Indirect immunofluorescence assay (IFA) using P. falciparum rhoptry specific antibodies showed intense reactivity with cytoplasmic vesicles of Colpodella …
Identifying Potential Causes Of Human Birth Defects Through Genetic Studies Of Worm Development, Solomiya Pushchak, Kaveri Khanna
Identifying Potential Causes Of Human Birth Defects Through Genetic Studies Of Worm Development, Solomiya Pushchak, Kaveri Khanna
Undergraduate Research Posters 2017
We will use the roundworm Caenorhabditis elegans to study the process of meiosis, which is involved in the formation of gametes (sperm and eggs). Its small size, rapid life cycle, transparency and well-annotated genome allows researchers to track the effects of mutations that disrupt gametogenesis. Our preliminary data demonstrated that a genetic screen for mutations that increase the viability of embryos produced by worms lacking SPO-11, a critical factor regulating meiotic chromosomal inheritance, can identify genes required for the accurate transmission of the genome from one generation to the next. To further test this hypothesis, we will continue this screen …
Expression Validation Of Mir-149-5p Targets In Prostate Cancer, Grant Wethington, Savita Singh
Expression Validation Of Mir-149-5p Targets In Prostate Cancer, Grant Wethington, Savita Singh
Undergraduate Research Posters 2017
Prostate cancer (PCa) is one of the leading causes of mortality in men. Current therapeutics for PCa are androgen depletion by castration or/and anti-androgen based treatments. Androgens are necessary for Androgen receptor (AR) to function as a transcription factor, AR then regulates the expression of genes which promote cancer cell proliferation. However, despite the therapeutic interventions recurred AR signaling, which is facilitated by the acquisition of mutations in AR and its amplification, cholesterol biosynthesis and alterations in the steroidogenesis continue promoting PCa carcinogenesis. Our research is focused on regulatory small RNA molecules know as microRNAs (miRNA) in PCa. Numerous miRNAs …
The Effects Of Cell Cycle Position On Skeletal Myoblast Differentiation Or Apoptosis, Asma Saleh, Briana Boslett
The Effects Of Cell Cycle Position On Skeletal Myoblast Differentiation Or Apoptosis, Asma Saleh, Briana Boslett
Undergraduate Research Posters 2017
Programmed cell death (apoptosis) is induced by the same culture conditions as differentiation in skeletal myoblasts, yet these processes result in mutually exclusive physiological endpoints. Dissecting this coordinate regulation could enable selective manipulation relevant to the alleviation of muscle degeneration, the effectiveness of regeneration, or treatment utilizing skeletal myoblast transfer. Analysis of asynchronous cultures of myoblasts in growth media (GM) were determined to have 50% of cells in G1 phase, 30% of cells in S phase and 20% of cells in G2/M phase. When skeletal myoblasts in GM are switched to differentiation media (DM), roughly 70% of cells undergo differentiation …
Phosphorus Variations In Lake Erie And Its Major Tributaries, Shiying Lu
Phosphorus Variations In Lake Erie And Its Major Tributaries, Shiying Lu
Undergraduate Research Posters 2017
As a recurring symptom of eutrophication in Lake Erie, massive blooms of harmful algae pose a threat to safe drinking water supply and recreational water use. Causes of the recent re-eutrophication in the lake include changes in the tributary phosphorus loading and/or increases in the internal nutrient loading potentially mediated by the colonization of zebra and quagga mussels. This study is to investigate the phosphorus variations in different nearshore locations of the lake and its major tributaries for a better understanding of the lake's trophic conditions. A total of 21 water samples were collected from Lake Erie and its major …
Purification And Crystallization Trials Of The Dihydroorotase From Methanococcus Jannaschii, Amy K. Dadisman
Purification And Crystallization Trials Of The Dihydroorotase From Methanococcus Jannaschii, Amy K. Dadisman
Undergraduate Research Posters 2017
Dihydroorotase is the enzyme that catalyzes the third step of the de novo biosynthesis of pyrimidines. M. jannaschii is a hyperthermophillic archaeon that can serve as a model organism for research purposes. This experiment is a first step toward elucidating the structure of the dihydroorotase in M. jannaschii. The enzyme was purified by salting out and heating the solution and then putting the supernatant through cation exchange chromatography and hydrophobic interaction chromatography. Twenty-four conditions were tested to determine if a crystal of dihydroorotase could be formed. Two of these conditions led to preliminary crystal formation. These findings can be utilized …