Life Sciences Commons^™

Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr

Maria Cekanova MS, RNDr, PhD

No abstract provided.

Ppar Agonists Down-Regulate The Expression Of Atp10c Mrna During Adipogenesis, A Peretich, Maria Cekanova Ms, Rndr, Phd, S Hurst, Sj Baek, Madhu Dahr

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

No abstract provided.

Methods And Compositions For Vaccnation Of Animals With Prrsv Antigens With Improved Immunogenicity, Israrul H. Ansari, Fernando A. Osorio, Asit K. Pattnaik,

School of Veterinary and Biomedical Sciences: Faculty Publications

Pigs challenged with hypoglycosylated variants of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) major surface protein GP5 exhibited increased production of PRRSV-neutralizing antibodies relative to the levels of neutralizing antibodies produced by pigs immunized with wild type (wt) or glycosylated GP5. This invention provides for methods of obtaining improved immune responses in pigs to PRRSV, compositions useful for obtaining the improved immune responses as well as isolated polynucleotides that encode hypoglycosylated variants of PRRSV major surface protein GP5.

Farm Focus - Fall 2009, Brian K. Whitlock, Matt Welborn, Maria Prado, Amy Plummer

Faculty Publications and Other Works -- Large Animal Clinical Sciences

No abstract provided.

South Dakota Thriving, Fall 2009, College Of Agriculture &. Biological Sciences

Growing South Dakota (Publication of the College of Agriculture, Food and Environmental Sciences)

Table of Contents:
[Page] 2 Growing Farmers Markets: South Dakota Cooperative Extension Service Helps Develop and Grow Booming Homegrown markets
[Page] 4 Lighari Leads Restructuring of Extension: Director of South Dakota Cooperative Extension Service Focuses on Building Relationships
[Page} 6 South Dakota's Newest Research Station: The Addition of the SDSU Cow Camp Means Another Tremendous Resource for South Dakota and SDSU
[Page] 8 SDSU Rodeo Builds World Champs: Focus on Academics and Team Leads to Success Inside Both the Classroom and the Arena
|[Page] 12 Behind the Scenes in SDSU's Anatomy Lab: SDSU's Hands-On Anatomy Lab Gives Students the Edge …

Single-Amino-Acid Alterations In A Highly Conserved Central Region Of Vesicular Stomatitis Virus N Protein Differentially Affect The Viral Nucleocapsid Template Functions, Debasis Nayak, Debasis Panda, Subash C. Das, Ming Lou, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The nucleocapsid protein (N) of vesicular stomatitis virus and other rhabdoviruses plays a central role in the assembly and template functions of the viral N-RNA complex. The crystal structure of the viral N-RNA complex suggests that the central region of the N protein interacts with the viral RNA. Sequence alignment of rhabdovirus N proteins revealed several highly conserved regions, one of which spanned residues 282 to 291 (GLSSKSPYSS) in the central region of the molecule. Alanine-scanning mutagenesis of this region suggested that replacement of the tyrosine residue at position 289 (Y289) with alanine resulted in an N-RNA template that is …

Farm Focus - Spring 2009, Brian K. Whitlock, Matt Welborn, Maria Prado, Amy Plummer

Faculty Publications and Other Works -- Large Animal Clinical Sciences

No abstract provided.

Biarsenical Labeling Of Vesicular Stomatitis Virus Encoding Tetracysteine-Tagged M Protein Allows Dynamic Imaging Of M Protein And Virus Uncoating In Infected Cells, Subash C. Das, Debasis Panda, Debasis Nayak, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

A recombinant vesicular stomatitis virus (VSV-PeGFP-M-MmRFP) encoding enhanced green fluorescent protein fused in frame with P (PeGFP) in place of P and a fusion matrix protein (monomeric red fluorescent protein fused in frame at the carboxy terminus of M [MmRFP]) at the G-L gene junction, in addition to wild-type (wt) M protein in its normal location, was recovered, but the MmRFP was not incorporated into the virions. Subsequently, we generated recombinant viruses (VSV-PeGFP-∆M-Mtc and VSV-∆M-Mtc) encoding Mprotein with a carboxy-terminal tetracysteine tag (Mtc) in place of the M protein. These recombinant viruses incorporated Mtc at levels similar to M in …

Effect Of Noxious Stimulation Upon Antipredator Responses And Dominance Status In Rainbow Trout, Paul J. Ashley, Sian Ringrose, Katie L. Edwards, Emma Wallington, Catherine R. Mccrohan, Lynne U. Sneddon

Aquaculture Collection

A potentially painful experience may modify normal behavioural responses. To gauge the importance of pain relative to predation or social status, we presented competing stimuli, a predator cue or an unfamiliar social group, to two groups of noxiously treated rainbow trout, Oncorhynchus mykiss. In the predator cue experiment, fish were classified as bold or shy. Noxiously stimulated fish did not show antipredator responses, suggesting that pain is the imperative. In the social status experiment, noxiously stimulated fish held individually and undisturbed showed an increase in respiration rate and plasma cortisol. As a comparison, we used the dominant or subordinate fish …

The Effects Of The Acetic Acid “Pain” Test On Feeding, Swimming, And Respiratory Responses Of Rainbow Trout (Oncorhynchus Mykiss): A Critique On Newby And Stevens (2008), Lynne U. Sneddon

Experimentation Collection

Newby and Stevens’ (2008) paper ‘‘The effects of the acetic acid ‘pain’ test on feeding, swimming, and respiratory responses of rainbow trout (Oncorhynchus mykiss)’’ examines the effects of a noxious stimulus on the behaviour of trout in an attempt to replicate research conducted in my laboratory (Sneddon, 2003a; Sneddon, 2007; Sneddon et al., 2003a,b; Reilly et al., 2008). However, the authors used a different protocol to the one already published and I would like to respond to some of their points of discussion to provide scientific explanations for their results using data from my laboratory. The authors show that swimming …

Utilización De La Haematobia Irritans Y Stomoxis Calcitrans Como Remedio Isopático En El Control Alternativo De Estos Ectoparásitos En Dos Hatos Lecheros Ubicados En El Departamento De Cundinamarca Colombia, Brayan Yersey Castro Rivera, Javier Alexis La Rotta Pineda

Medicina Veterinaria

El presente estudio se baso en la utilización de la Haemotobia irritans y Stomoxys calcitrans como remedio isopático para el control de estos ectoparásitos. El trabajo se llevo a cabo en dos haciendas dedicadas a la producción láctea especializada, la primera fue la hacienda Liswia y la segunda la Huerta, ubicadas en los municipios de Gachancipá y Facatativá (Cundinamarca, Colombia) respectivamente, el grupo de estudio conto con un numero total de 60 bovinos hembras de raza holstein friesian, en las cuales se aplicaron dos tratamientos T1 y T2, respectivamente en cada finca, además cada tratamiento conto con 15 repeticiones, en …

Group Feeding For The Lactating Cow, Anthony Joseph Martin

Dairy Science

Feeding individualized rations for lactating dairy cattle in the commercial herd based on dietary needs is a popular issue. This has been a debating topic since evolution of the Total Mixed Ration (TMR). Since dairymen were able to mix grain in a feed wagon and mix a specific dietary ration to meet the demand for an organized group, there has been an issue in which protocol cow pen grouping should take. Dairymen have always feed cattle based on if lactating or late in their gestation, nonlactating. With the evolution of the TMR dairymen are then allowed to go into more …

Feedyard Assessment: Assessor's Guide

Nebraska Beef Quality Assurance Program

The BQA Feedyard Assessment is an on-site educational tool that allows for assessing and benchmarking key indicators of animal care and well-being as well as feedyard conditions. The Feedyard Assessment focuses on three main areas – Animals, Records and Best Management Practices (BMP), and Facilities and Equipment.

Stocker And Backgrounding Self Assessment

Nebraska Beef Quality Assurance Program

Cattlemen in stocker operations have long recognized the need to properly care for livestock. Sound animal husbandry practices, based on decades of practical experience and research, are known to impact the well-being of stocker cattle, individual animal health and herd productivity. Stocker cattle operations are located in many diverse environments and geographic locations in the United States. A universal set of production practices can not be recommended for all stocker cattle producers in light of the divergent climate and geographic variations of the stocker and backgrounding segment. Personal experience, training and professional judgment can serve as a valuable resource for …

Somatic Cell Nuclear Transfer In Zebrafish, Kannika Siripattarapravat, Patrick J. Venta, C C. Chang, Jose B. Cibelli

Jose Cibelli

We developed a method for somatic cell nuclear transfer in zebrafish using laser-ablated metaphase II eggs as recipients, the micropyle for transfer of the nucleus and an egg activation protocol after nuclear reconstruction. We produced clones from cells of both embryonic and adult origins, although the latter did not give rise to live adult clones.

Farm Focus - Spring 2009, Brian K. Whitlock, Matt Welborn, Maria Prado, Amy Plummer

Brian K Whitlock, PhD, DVM, DACT

No abstract provided.

Farm Focus - Fall 2009, Brian K. Whitlock, Matt Welborn, Maria Prado, Amy Plummer

Brian K Whitlock, PhD, DVM, DACT

No abstract provided.

Prevalence Of Intestinal Parasite Infection In Symptomatic And Asymptomatic Dogs In Southwestern West Virginia: The Potential Impact On Human Health, Tashina Marie Savilla

Theses, Dissertations and Capstones

Most dog owners are unaware of asymptomatic infection and of the possibility of contracting zoonotic parasites from their dogs. We hypothesized that parasite infection is: associated with one or more symptoms; independent of gender and age; and independent of anthelmintic usage. Stool samples were collected from 231 dogs in Kanawha County, West Virginia, and were examined by simple fecal flotation. Parasitic prevalence was found to be 23% for Ancylostoma caninum, 8% for Trichuris vulpis, 7% for Toxocara canis, 4% for Isospora species, and 32.5% overall. There was no significant relationship between infection and gender, nor between infection and symptom for …

Effect Of Leptin Gene Polymorphisms On Milk Production Traits Of Jersey Cows, Hanna Kulig, Marek Kmiec, Inga Kowalewska Luczak, Gabriela Andziak

Turkish Journal of Veterinary & Animal Sciences

A total of 181 Jersey cows were used to investigate how leptin gene polymorphisms affect milk production traits such as milk yield, fat and protein yield, and fat and protein content. Two single-nucleotide polymorphisms (SNPs) were genotyped, including the A59V in exon 3 and Sau3AI in intron 2. The genotype, allele, and haplotype frequencies were estimated. Statistical analysis revealed that the A59V polymorphism significantly affected the milk, protein, and fat yield (P Keywords: Leptin, SNP, Jersey cows, milk production traits

Surgical Correction Of Small Colon Faecalith In A Dare-Shuri Foal, Behrooz Nikahval, Nasser Vesal, Mohsen Ghane

Turkish Journal of Veterinary & Animal Sciences

A 6-month-old male Dare-Shuri foal was admitted for acute abdominal pain unresponsive to medical treatment. The foal was anxious and exhibiting signs of abdominal pain. The foal had not defecated in the previous 5 days and severe abdominal distension was present. Based on clinical signs and the results of the diagnostic work-up, gastrointestinal obstruction was suspected. Midline exploratory celiotomy revealed a faecalith in the small colon. A longitudinal incision was made through the antimesenteric border of the involved intestine and an approximately 350 g mass mostly composed of dried roughage was removed. The day after surgery, intestinal sounds were detectable; …

Nuclear Morphometry In Relation To Tumor Grade In Canine Spontaneous Cutaneous Squamous Cell Carcinomas, Radostin Simeonov

Turkish Journal of Veterinary & Animal Sciences

In a retrospective study on cytological specimens from 17 dogs with histologically confirmed cutaneous squamous cell carcinoma, the morphometric variables were studied and compared to tumors' nuclear grade. The morphometric parameters evaluated in this study were mean nuclear area (MNA, µm2), mean nuclear perimeter (MNP, µm), mean nuclear diameter (D mean, µm), minimum nuclear diameter (D min, µm), and maximum nuclear diameter (D max, µm). Associations between MNA, MNP, D mean, D min, and D max and tumors' grade were assessed using the ANOVA/LSD test (Statistica 6.0, StatSoft, USA) at a level of significance of P < 0.05. The result indicated an increase in the mean values of the nuclear parameters with increasing tumor grade. In the cases examined, significant differences in MNA, MNP, and D mean occurred between tumors in all grades. Besides, no significant differences in D max (between grade I-II and II-III) and D min (grade II-III) were found. In conclusion, nuclear morphometry could be used as an additional tool for differentiating nuclear grade in canine cutaneous squamous cell carcinomas.

Volume 6, Number 2 (2009), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Developing Safer Sports Turfs

Volume 6, Number 1 (2009), Ut Institute Of Agriculture

Tennessee Land, Life and Science Magazine

Issue Highlights:

• Innovations in Learning

The Detection And Analysis Of Selected Pathogens In The Water Supply Of Dairy Cohort Herds., Niall Drummond

Theses

The issue of food safety is a growing concern to Irish agriculture and to the food industry. In the last twenty years, many novel pathogens, which can cause concern to the food industry, have been identified. Organisms such as Verocytotoxigenic Escherichia coli (VTEC) cause hemorrhagic colitis and Haemolytic Uremic Syndrome (HUS), which is the major cause of acute renal failure in children in many countries, and it’s presence in food is a cause of growing concern. Other pathogens causing concern are those implicated as causative agents of Acute Gastroenteritis (AGE) and these can be bacterial, viral and parasitic organisms.

Consumption …

Effect Of Docking And Diet Energy On Carcass Fat Characteristics In Fat-Tailed Baluchian Sheep, Ali̇ Moharrery

Turkish Journal of Veterinary & Animal Sciences

Effects of docking on carcass fat characteristics were studied in fat-tailed sheep. Male lambs were randomly divided into 2 groups. One group was docked after birth using rubber rings, and the tails of the other group were left intact (control). After weaning, 20 lambs from each group were divided into 2 subgroups; one subgroup was fed with a normal energy diet (10.25 MJ/kg ME) and the other one with a high-energy diet (11.42 MJ/kg ME) for 84 days. At the end of the fattening period, 28 lambs were slaughtered for determination of warm carcass weight (WCW) and other carcass characteristics. …

Nutritive Value Of Damascus Rose Extraction Residue Ensiled With Different Effluent Absorbents, Hassan Fazaeli, Mojtaba Zahedifar

Turkish Journal of Veterinary & Animal Sciences

Two experiments were conducted to study the silage characteristics and nutritive value of Damascus rose extraction residue (RER) which is a by-product remained after the extraction of rose water in some parts of Iran. In the first experiment, RER was ensiled together with different amounts of effluent absorbents, using a completely randomised design with 10 treatments and 4 replicates. In the second experiment, RER was ensiled together with dry sugar beet pulp, wheat straw, and limestone. The contents of OM, CP, CF, EE, and NFE were 94.4%, 11%, 24%, 24%, and 57.3%, respectively, in the initial samples of RER. The …

Effect Of Phosalone On Haematological Indices In The Tilapia, Oreochromis Mossambicus, H. Abdul Jaffar Ali, V. Jaya Rani

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to assess the effect of phosalone on the tilapia, Oreochromis mossambicus. The effect was assessed on the basis of the results of acute toxicity tests and comparison of results of haematological examination of a control and an experimental group exposed to phosalone. The 96 h LC_{50} is the basic value in the acute toxicity test and it was 0.26 mg/L for phosalone against the test fish. Haematological profile of the healthy fish was carried out with 2 sublethal concentrations, viz 0.0325 mg/L (1/8) and 0.065 mg/L (1/4) of phosalone. The experiment was performed semi-statically …

Ameliorative Effect Of Esterified Glucomannan, Sodium Bentonite, And Humic Acid On Humoral Immunity Of Broilers During Chronic Aflatoxicosis, Hasan Ghahri, Ali̇reza Talebi, Mohamad Chamani, Houshang Lotfollahian, Nazar Afzali

Turkish Journal of Veterinary & Animal Sciences

A study was conducted on the impact of aflatoxin (AF) and mycotoxin binders on immunization against Infectious Bursal Disease (IBD) and Infectious Bronchitis (IB) in chickens. Broiler chicks (7-day-old) were randomly assigned to 9 dietary treatments with 4 replicates of 12 chicks each. Treatments were 1) control; 2) diet containing corn naturally contaminated with AF (NCD); 3) NCD + 0.2% Farmagülatör DRYTM humate (FH); 4) NCD + 0.4% FH; 5) NCD + 0.6% FH; 6) NCD + 0.8% FH; 7) NCD + 1% FH; 8) NCD + 0.5% sodium bentonite (SB); and 9) NCD + 0.1% esterified glucomannan (E-GM). Data …

Association Of Polymorphisms In The Intron 1 Of Duck Prolactin With Egg Performance, Hui Fang Li, Wen Qi Zhu, Kuan Wei Chen, Tang Jie Zhang, Wei Tao Song

Turkish Journal of Veterinary & Animal Sciences

Gaoyou duck (Chinese indigenous breed) is famous for double-yolked egg. In the present study, SNP of prolactin (PRL) gene intron 1 of Gaoyou duck was detected by PCR-RFLP and DNA sequencing. The results indicated that there were 2 Dra I recognition sites in intron 1 of the PRL gene, but only 1 was of polymorphism. A T/C mutation at the position of the 1326 bp of the PRL gene was found by DNA sequencing, and resulted in 3 genotypes AA, AB, and BB. The frequencies of genotype BB and allele B were the highest. The association analysis between the polymorphism …

Detection Of Bovine Tuberculosis By Tuberculin Test And Polymerase Chain Reaction In Van, Turkey, Hasan Solmaz, Zi̇ya İlhan, Abdulbaki̇ Aksakal, Ti̇mur Gülhan, İsmai̇l Hakki Eki̇n

Turkish Journal of Veterinary & Animal Sciences

The aim of this study was to identify the presence and prevalence of bovine tuberculosis in the eastern Anatolian border province of Van and its villages and to collect the preliminary data required for programs for the prevention and control of this zoonotic disease. The tuberculin test was performed on 210 cattle. Nasal swab and milk samples were obtained from the animals tested. Three cattle that had a positive tuberculin test also tested positive for the DNA target, a 580-bp fragment of IS6110 specific for members of the Mycobacterium tuberculosis complex. This fragment was also recovered from milk taken from …