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Plant Biology

Nitrogen

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Full-Text Articles in Life Sciences

Perennial Grass Emergence And Establishment Using A Micro-Nutrient Seed Treatment, Charlie D. Clements, J. A. Young Jun 2023

Perennial Grass Emergence And Establishment Using A Micro-Nutrient Seed Treatment, Charlie D. Clements, J. A. Young

IGC Proceedings (1997-2023)

Resource managers have become increasingly frustrated with restoration seeding failures in semi-arid and arid environments. In response to this frustration, some resource managers have attempted restoration seedings using non-conventional methodologies such as propriety seed treatments. The exact nature of these propriety treatments is often confidential, but they generally consist of either nutrient or micro-nutrient enrichment or inoculation with unspecified micro-organisms. One of the more popular propriety seed treatment used in Nevada, USA, is GERM-N-8®. This product is a suspension of nutrients (N 2%, P 14%, and K 3%) applied to dry seed. Resource managers often report excellent success using these …


Effect Of Dairy Effluent On Turnip Yields, Joe L. Jacobs, G. N. Ward, Frank R. Mckenzie Jun 2023

Effect Of Dairy Effluent On Turnip Yields, Joe L. Jacobs, G. N. Ward, Frank R. Mckenzie

IGC Proceedings (1997-2023)

Dairy effluent is a significant point source in the pollution of waterways. Only 50% of dairy farms in the dryland regions of Victoria, Australia, have suitable dairy effluent systems of which only 25% are managed effectively (IRIS Research 2000). Despite many farmers viewing effluent as an undesirable waste, it contains relatively large amounts of agronomically valuable nutrients especially nitrogen (N) and potassium (K). Results are reported from the first two years of a three year study comparing turnip leaf and root dry matter (DM) responses to a range of dairy effluent rates.


The Input Of Forage Legumes In Sustainable Grassland Systems, Žydrė Kadžiulienė, L. Sarunaite Jun 2023

The Input Of Forage Legumes In Sustainable Grassland Systems, Žydrė Kadžiulienė, L. Sarunaite

IGC Proceedings (1997-2023)

There is increased interest in sustainable grassland systems. One step towards sustainability is expansion of legume use, because of their potential to fix and transfer nitrogen (N) to subsequent crops. However, legumes can also have negative aspects, such as difficulties in establishment (Porqueddu et al., 2003), lack of persistence, N loss (Scholefield et al., 2002) and accumulation of soil borne disease agents (Kadziulis, 2001). The large variability within legume swards and between years in pastures and leys has encouraged us to search for possibilities to achieve stability of their inputs in sustainable grassland systems.


A Rapid Estimation Of Nitrogen Bound To Neutral Detergent Fibre In Forages By Near Infrared Reflectance Spectroscopy, Jérôme Bindelle, G. Sinnaeve, P. Dardenne, P. Leterme, A. Buldgen Jun 2023

A Rapid Estimation Of Nitrogen Bound To Neutral Detergent Fibre In Forages By Near Infrared Reflectance Spectroscopy, Jérôme Bindelle, G. Sinnaeve, P. Dardenne, P. Leterme, A. Buldgen

IGC Proceedings (1997-2023)

Near infrared reflectance spectroscopy (NIRS) is widely used as a rapid method for the evaluation of the chemical composition or the nutritive value of foodstuffs (Givens et al., 1997). The determination of the neutral detergent fibre (NDF) bound N (NDF-N), which is highly variable in forages (Shayo & Udén, 1999), is expensive. The purpose of this study was to test the use of NIRS in the prediction of NDF-N in various forages.


The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan May 2023

The Effect Of Nitrogen Fertiliser And Season On The In Situ Degradability Of Irish Perennial Ryegrass In Cattle, V. Olsson, J. J. Murphy, F. P. O'Mara, K. O'Connell, J. Humphreys, F. J. Mulligan

IGC Proceedings (1997-2023)

In light of increasing environmental and economic pressure on agriculture to utilise resources more efficiently, protein feeding and its effects are fundamentally important. As grazed grass is the predominant feed in Irish dairy and beef cattle production systems, it is necessary to establish protein values for different grass varieties and cultivars fed. It is also important to investigate the extent of ruminal nitrogen (N) degradability for these grasses since this characteristic greatly influences environmentally damaging urinary N excretion.


Modelling Of Nitrogen Allocation And Partitioning Within Lucerne (Medicago Sativa) Shoot Tissues During Recovery From Defoliation: An Approach To Estimate Forage Production And Nitrogen Composition, F. Meuriot, A. Escobar-Gutiérrez, J-C. Avice, J-C. Simon, F. Lesuffleur, F. Gastal May 2023

Modelling Of Nitrogen Allocation And Partitioning Within Lucerne (Medicago Sativa) Shoot Tissues During Recovery From Defoliation: An Approach To Estimate Forage Production And Nitrogen Composition, F. Meuriot, A. Escobar-Gutiérrez, J-C. Avice, J-C. Simon, F. Lesuffleur, F. Gastal

IGC Proceedings (1997-2023)

Lucerne has been grown over centuries for forage. Its forage production is strongly correlated to the initial taproot and stubble N reserves (Avice et al., 1996; Meuriot et al., 2004). However, the influence of cutting management on the level of N storage and the contribution of these N reserves to forage production still remain unclear and need to be studied at the whole plant level. For this purpose, a deterministic model of N allocation within the different organs and partitioning within different biochemical N pools was developed for lucerne with high and low initial N status and cutting …


Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo May 2023

Exploring Gene Expression Patterns For Resilient Maize Lines Under Nitrogen Stress, Alice Guo

Honors Theses

Nitrogen plays a major role in the proper growth and development of maize and is therefore essential to crop production, being the most critical nutrient for achieving optimal yield. In previous field and greenhouse studies, we have found maize lines that differ in their resiliency to nitrogen stress. To identify the potential genomic regions associated with the differences in nitrogen deficiency resilience, Genome-Wide Association Studies (GWAS) were conducted. Based on a previous study, the most consistently resilient maize inbred lines within the Goodman-Buckler diversity panel have been identified as the lines A619 and A661, while the non-resilient inbred lines have …


Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders May 2023

Plasticity Of Sorghum Biomass And Inflorescence Traits In Response To Nitrogen Application, Kyle M. Linders

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen is an essential nutrient required for growth and development in plants. Insufficient nitrogen availability can reduce vegetative growth and grain yield. However, nitrogen is a costly input for farmers, is energy intensive to manufacture, and runoff of excess nitrogen fertilizer impacts water quality. Compared to its close relative, maize, sorghum has much greater resilience to nitrogen and water deficit, and heat stress, allowing sorghum to be grown with fewer inputs and on marginal land. Variation in total biomass accumulation and grain yield between sorghum accessions, as well as between nitrogen conditions, can be largely explained by differences in vegetative …


Urea Applied To Puccinellia-Based Pastures Increases Pasture And Sheep Production, M. L. Hebart, N. J. Edwards, A. D. Craig, E. A. Abraham, J. D. Mcfarlane, J. E. Hocking Edwards Apr 2023

Urea Applied To Puccinellia-Based Pastures Increases Pasture And Sheep Production, M. L. Hebart, N. J. Edwards, A. D. Craig, E. A. Abraham, J. D. Mcfarlane, J. E. Hocking Edwards

IGC Proceedings (1997-2023)

In the 1950's large areas of native vegetation in the upper south east of South Australia (SA) were replaced with highly productive Hunter River lucerne. This maintained groundwater recharge at near pre-clearing levels. The area of lucerne was reduced dramatically in the late 1970's by a combination of lucerne aphids, wingless grasshoppers and drought. In 1981 severe flooding inundated large areas of the region, causing the saline groundwater to rise to the soil surface. Since that time, dryland salinity has been a feature of the local farming system and salt-tolerant pastures based on puccinellia (Puccinellia ciliata) were widely …


Effect Of Nitrogen On The Radiation Use Efficiency For Modelling Grass Growth, R. Lambert, A. Peeters Feb 2023

Effect Of Nitrogen On The Radiation Use Efficiency For Modelling Grass Growth, R. Lambert, A. Peeters

IGC Proceedings (1997-2023)

When nitrogen (N) is not at a sufficient level to permit maximum growth rate, dry matter production is reduced. Models of plant growth in relation to solar radiation intercepted by the crop have been largely used. According to these models, N deficiency can act on the leaf extension and thus on the quantity of radiation intercepted by the crop, but also by reducing the radiation use efficiency of the crop (RUE) (Bélanger, 1990). The effect of N on the RUE of ryegrass swards is determined and discussed.


Radiation Use Efficiency Of Ryegrass: Determination With Non Cumulative Data, R. Lambert, A. Peeters Feb 2023

Radiation Use Efficiency Of Ryegrass: Determination With Non Cumulative Data, R. Lambert, A. Peeters

IGC Proceedings (1997-2023)

The growth of a crop is generally described as biomass accumulation per unit time. Monteith (1977) developed a model of growth where biomass accumulation is related to solar radiation intercepted by the crop. This model has been largely used for different crops. The conversion factor between radiation absorbed or intercepted by the crop and the biomass production is called “radiation use efficiency” or “dry matter radiation quotient”. Radiation use efficiency (RUE) is usually calculated as the regression coefficient of the linear relationship between crop biomass measured repeatedly during growth and cumulated intercepted or absorbed solar radiation. Demetriades-Shah et al. …


The Effect Of Grassland Management On Bovine Nitrogen Efficiency, N. J. Hoekstra, R. P. O. Schulte, E. A. Lantinga, P. C. Struik Feb 2023

The Effect Of Grassland Management On Bovine Nitrogen Efficiency, N. J. Hoekstra, R. P. O. Schulte, E. A. Lantinga, P. C. Struik

IGC Proceedings (1997-2023)

Nitrogen (N) losses through grazing bovines are at the heart of the current debate on environment and agriculture. N utilisation of grazing bovines is predominantly determined by the form and amount of energy and protein in their diet, which in Ireland consists mainly of grazed grass. The two main problems of grazed grass with respect to animal N utilisation are 1) the imbalance between total N content and energy content, and 2) the lack of synchronisation between the release of N and carbohydrates in the rumen. It was hypothesised that both the balance and synchronisation of N and energy in …


Potential Yield Of Cocksfoot (Dactylis Glomerata) Monocultures In Response To Irrigation And Nitrogen, A. Mills, D. J. Moot, R. L. Lucas, P. D. Jamieson, B. A. Mckenzie Feb 2023

Potential Yield Of Cocksfoot (Dactylis Glomerata) Monocultures In Response To Irrigation And Nitrogen, A. Mills, D. J. Moot, R. L. Lucas, P. D. Jamieson, B. A. Mckenzie

IGC Proceedings (1997-2023)

Cocksfoot is a widely sown grass in temperate pastures. However, while potential yield of cocksfoot can exceed 28 t DM/ha per year, it is often restricted by water, temperature and nitrogen (N). Of these, Peri et al. (2002) showed that N was severely limiting in all seasons. The aim of this study was to confirm the potential yield of cocksfoot and quantify the extent of yield reductions due to environmental constraints.


Blood Meal As A Source Of Histidine For Cattle Fed Grass Silage And Barley, R. Berthiaume, C. Lafrenière Feb 2023

Blood Meal As A Source Of Histidine For Cattle Fed Grass Silage And Barley, R. Berthiaume, C. Lafrenière

IGC Proceedings (1997-2023)

Previous research has shown that cattle fed grass silage are responsive to protected amino acids (Veira et al., 1991). Methionine and lysine were suggested as the most limiting amino acids for grass silage diets. Recently, Korhonen et al. (2000) have shown that histidine is the first limiting amino acids for dairy cows fed grass silage and barley-based concentrates. However, histidine is not available in a rumen protected form and needs to be provided through dietary ingredients. Blood meal is rich in histidine. This trial was designed to determine the effect of increasing histidine supply through blood meal on …


Grassland Productivity And Water Quality: A 21st Century Issue, David M. Nash, P. M. Haygarth Jan 2023

Grassland Productivity And Water Quality: A 21st Century Issue, David M. Nash, P. M. Haygarth

IGC Proceedings (1997-2023)

  1. Irrigation and other changes to the hydrological cycle can increase soil and water salinity.
  2. Primary salinisation is a natural process that affects much of Europe, Asia, Africa, the Americas and Australia. Secondary salinisation is caused by human activities such as irrigation and land clearing that mobilise salt stored in the soil.
  3. The critical water contaminants exported from grasslands are nitrogen, phosphorus, potential pathogens and sediment.
  4. The mechanisms responsible for diffuse pollution from grasslands and mitigation strategies are most effectively investigated using a ‘source-mobilisation-transport’ framework.
  5. There is a lack of coherent interaction across discipline boundaries that links pollutant sources to impact. …


Nitrogen Transfer From Cover Crops To The Subsequent Grain Crop And The Influence Of Variability In Winter Conditions, Spencer Heuchan Dec 2022

Nitrogen Transfer From Cover Crops To The Subsequent Grain Crop And The Influence Of Variability In Winter Conditions, Spencer Heuchan

Electronic Thesis and Dissertation Repository

Cover crops, which are used to provide ground cover after the harvest of the grain crop, can potentially improve the sustainability of agroecosystems by reducing nutrient losses. However, few data are available to document the extent to which cover crops improve both the retention of soil nitrogen (N) and the transfer of this N to the grain crop. The efficiency of this N transfer may be further influenced by variation in winter soil temperature; for example, reduced snow cover can increase the frequency and intensity of soil freezing, which can affect the survival of cover crops and the timing of …


Nitrification Inhibitor, Nitrogen Source, And Herbicide Effects On Soil Nitrogen Transformations And Corn Yield, William Neels Dec 2022

Nitrification Inhibitor, Nitrogen Source, And Herbicide Effects On Soil Nitrogen Transformations And Corn Yield, William Neels

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nitrogen fertilizer management continue to be challenging due to potential nitrogen losses under variable weather conditions. The objective of this was to evaluate the performance of nitrification inhibitors, nitrogen sources and herbicides on in-season nitrogen availability and agronomy indicators. A two site-year field experiment was conducted in silty clay loam soil in corn phase of the corn-soybean rotation at Central Nebraska. The treatments included three herbicide (no pre-emergence, Acuron, Resicore) and five nitrogen treatments: 1) control, 2) anhydrous ammonia, 3) anhydrous ammonia without nitrification inhibitor, 4) urea with nitrification inhibitors, and 5) urea without nitrification inhibitors. Results indicated that nitrogen …


Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson Aug 2022

Mycorrhizae In Maize (Zea Mays L.) Cropping Systems Respond Differently To Nitrogen Fertilization Under Increasing Crop Rotational Diversity, Morgan Mcpherson

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Arbuscular mycorrhizal fungi (AMF) remain a vital obligate symbiont of nearly all plants. It is well established that the symbiosis between AMF and host plant improves plant nutrient acquisition, alleviates abiotic and biotic environmental stressors, defends against plant pathogens, and contributes to overall plant fitness and productivity through modification of the soil habitat. Modifications include increased soil aggregation and stability, carbon sequestration through provision of fungal wall precursors to soil organic matter (SOM) formation, and enhanced nutrient cycling in the mycorrhizosphere. The goal of this dissertation was to assess how AMF respond to nitrogen (N) fertilization regimes in maize cropping …


Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell Feb 2022

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …


Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell Feb 2022

Post-Termination Effects Of Cover Crop Monocultures And Mixtures On Soil Inorganic Nitrogen And Microbial Communities On Two Organic Farms In Illinois, Eleanor E. Lucadamo, Ashley A. Holmes, Samuel E. Wortman, Anthony C. Yannarell

Department of Agronomy and Horticulture: Faculty Publications

Cover crops can continue to affect agricultural systems even after they have been terminated by influencing nitrogen dynamics and by altering soil microbial communities. These post-termination effects can influence soil fertility, weed pressure, and the dynamics of potential plant pathogens in the narrow window of time between cover crop termination and cash crop emergence. We evaluated the post-termination effects of 12 different spring-sown cover crop mixtures and monocultures on soil nitrogen and microbial communities on two different organic farms in Central Illinois (on Lawson silt loam soil) and Northern Illinois (on Virgil silt loam soil). In comparison to control plots …


Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini Jan 2020

Insufficient Nitrogen Supply From Symbiotic Fixation Reduces Seasonal Crop Growth And Nitrogen Mobilization To Seed In Highly Productive Soybean Crops, Nicolas Cafaro La Menza, Juan Pablo Monzon, John L. Lindquist, Timothy J. Arkebauer, Johannes M.H. Knops, Murray Unkovich, James E. Specht, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) supply can limit the yields of soybean [Glycine max (L.) Merr.] in highly productive environments. To explore the physiological mechanisms underlying this limitation, seasonal changes in N dynamics, aboveground dry matter (ADM) accumula- tion, leaf area index (LAI) and fraction of absorbed radiation (fAPAR) were compared in crops relying only on biological N2 fixation and available soil N (zero-N treatment) versus crops receiving N fertilizer (full-N treatment). Experiments were conducted in seven high-yield environments without water limitation, where crops received optimal management. In the zero-N treatment, biological N2 fixation was not sufficient to meet the N demand of …


Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn, Laura J. Thompson, Laila A. Puntel Jan 2020

Transforming Unmanned Aerial Vehicle (Uav) And Multispectral Sensor Into A Practical Decision Support System For Precision Nitrogen Management In Corn, Laura J. Thompson, Laila A. Puntel

Department of Agronomy and Horticulture: Faculty Publications

Determining the optimal nitrogen (N) rate in corn remains a critical issue, mainly due to unaccounted spatial (e.g., soil properties) and temporal (e.g., weather) variability. Unmanned aerial vehicles (UAVs) equipped with multispectral sensors may provide opportunities to improve N management by the timely informing of spatially variable, in-season N applications. Here, we developed a practical decision support system (DSS) to translate spatial field characteristics and normalized difference red edge (NDRE) values into an in-season N application recommendation. On-farm strip-trials were established at three sites over two years to compare farmer’s traditional N management to a split-application N management guided by …


Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini Jan 2020

Benchmarking Impact Of Nitrogen Inputs On Grain Yield And Environmental Performance Of Producer Fields In The Western Us Corn Belt, Fatima A.M. Tenorio, Eileen L. Mclellan, Alison J. Eagle, Kenneth G. Cassman, Daryl Andersen, Marie Krausnick, Russell Oaklund, John Thorburn, Patricio Grassini

Department of Agronomy and Horticulture: Faculty Publications

Benchmarking crop yields against nitrogen (N) input levels can help provide opportunities to improve N ferti-lizer efficiency and reduce N losses on maize in the US Corn Belt by identifying fields most likely to benefit from improved N management practices. Here, we evaluated a large producer database that includes field-level data on yield and applied N inputs from 9280 irrigated and rainfed fields over a 7-year period (2009–2015) in Nebraska (USA). A spatial framework, based on technology extrapolation domains, was used to cluster each field into spatial units with similar climate and soil type that represent 1.3 million ha of …


Effects Of Different Water And Nitrogen Regimens On Yield Of Winter Wheat Produced In Nebraska, Joseph Emory Davis Dec 2019

Effects Of Different Water And Nitrogen Regimens On Yield Of Winter Wheat Produced In Nebraska, Joseph Emory Davis

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Wheat is the 3rd most prominent crop in the USA and approximately 50% is exported annually. Nebraska wheat production is 11th in the country, and it plays a major role in the state's agricultural economy, especially in western NE. Generally, wheat is grown under dryland conditions and the region grows much more wheat on unirrigated land than it does on irrigated. However, deficit irrigation has shown great value in producing high yielding wheat with much less water than needed for other crops. Finding new ways to leverage irrigation in wheat production may help address the need to produce food …


Understanding Nitrogen Limitation In Soybean, Nicolas Cafaro La Menza Dec 2019

Understanding Nitrogen Limitation In Soybean, Nicolas Cafaro La Menza

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Meeting soybean demand on existing cropland area for a global population of 9.7 billion people by the year 2050 requires narrowing the existing gap between average producer yield and yield potential. Soybean relies on two sources on nitrogen (N): biological N2 fixation and indigenous soil N supply. As soybean yield continues to increase, it seems critical to know if there is a yield level at which potential contribution of indigenous nitrogen sources and fixation becomes insufficient to meet crop N requirements for high yields, while still maintaining or increasing protein and oil concentration. This study evaluated N limitation across 29 …


The Effect Of Preflood Nitrogen And Flood Establishment Timing On Rice Development, Nitrogen Uptake And Grain Yield, Tyler Richmond Dec 2017

The Effect Of Preflood Nitrogen And Flood Establishment Timing On Rice Development, Nitrogen Uptake And Grain Yield, Tyler Richmond

Graduate Theses and Dissertations

Urea-N fertilizer is typically applied at the 5-leaf stage to rice (Oryza sativa L.) grown in a dry-seeded, delayed-flood production system. How long the preflood-N can be delayed without adverse effects on yield potential is poorly understood. The research objective was to determine the effects of preflood-N application and flood establishment timing on aboveground-N content, 50% heading, yield components, and grain yield. Trials were established on silt loam soils at the Pine Tree Research Station (PTRS) and Rice Research and Extension Center (RREC) during 2015 and 2016. Urea-N was applied at 0, 45, 90, 135, and 180 kg N ha-1 …


Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold Oct 2017

Effects Of Nitrogen And Phosphorus Fertilizer And Topsoil Amendment On Native Plant Cover In Roadside Revegetation Projects, Heidi L. Hillhouse, Walter H. Schacht, Jonathan M. Soper, Carol E. Wienhold

Department of Agronomy and Horticulture: Faculty Publications

Establishing vegetation on roadsides following construction can be challenging, especially for relatively slow growing native species. Topsoil is generally removed during construction, and the surface soil following construction (“cut-slope soils”) is often compacted and low in nutrients, providing poor growing conditions for vegetation. Nebraska Department of Transportation (NDOT) protocols have historically called for nitrogen (N) and phosphorus (P) fertilization when planting roadside vegetation following construction, but these recommendations were developed for cool-season grass plantings and most current plantings use slower-establishing, native warmseason grasses that may benefit less than expected from current planting protocols. We evaluated the effects of nitrogen and …


Population-Level Coordination Of Pigment Response In Individual Cyanobacterial Cells Under Altered Nitrogen Levels, Jaclyn Murton, Aparna Nagarajan, Amelia Y. Nguyen, Michelle Liberton, Harmony A. Hancock, Himadri B. Pakrasi, Jerilyn A. Timlin Jul 2017

Population-Level Coordination Of Pigment Response In Individual Cyanobacterial Cells Under Altered Nitrogen Levels, Jaclyn Murton, Aparna Nagarajan, Amelia Y. Nguyen, Michelle Liberton, Harmony A. Hancock, Himadri B. Pakrasi, Jerilyn A. Timlin

Biological Sciences Faculty Publications

Cyanobacterial phycobilisome (PBS) pigment-protein complexes harvest light and transfer the energy to reaction centers. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. We characterized the response of four key photosynthetic pigments to nitrogen depletion and repletion at the subcellular level in individual, live Synechocystis sp. PCC 6803 cells using hyperspectral confocal fluorescence microscopy and multivariate image analysis. Our results revealed that PBS degradation and re-synthesis comprise a rapid response to …


Propagation And Container Production Of The Amelanchier Spicata Complex, Gregory J. Melcher Aug 2016

Propagation And Container Production Of The Amelanchier Spicata Complex, Gregory J. Melcher

Electronic Theses and Dissertations

Amelanchier is a genus in the Rosaceae containing shrubs and trees indigenous to North America that possess traits valued by the horticulture industry. Amelanchier spicata (dwarf serviceberry), a heterogeneous complex of hybrids indigenous to eastern North America, has agricultural and ornamental merit with notable characteristics. White blossoms emerge in early spring, edible pomes ripen in the summer, and vibrant, orange foliage occurs in the fall. I investigated phenological and physiological factors influencing adventitious rooting of stem cuttings, the effects of nitrogen source on the development of seedlings grown in container culture, and phenotypic variation among seedlings of A. spicata from …


Expression Of A Constitutively Active Nitrate Reductase Variant In Tobacco Reduces Tobacco-Specific Nitrosamine Accumulation In Cured Leaves And Cigarette Smoke, Jianli Lu, Leichen Zhang, Ramsey S. Lewis, Lucien Bovet, Simon Goepfert, Anne M. Jack, James D. Crutchfield, Huihua Ji, Ralph E. Dewey Jun 2016

Expression Of A Constitutively Active Nitrate Reductase Variant In Tobacco Reduces Tobacco-Specific Nitrosamine Accumulation In Cured Leaves And Cigarette Smoke, Jianli Lu, Leichen Zhang, Ramsey S. Lewis, Lucien Bovet, Simon Goepfert, Anne M. Jack, James D. Crutchfield, Huihua Ji, Ralph E. Dewey

Kentucky Tobacco Research and Development Center Faculty Publications

Burley tobaccos (Nicotiana tabacum) display a nitrogen-use-deficiency phenotype that is associated with the accumulation of high levels of nitrate within the leaf, a trait correlated with production of a class of compounds referred to as tobacco-specific nitrosamines (TSNAs). Two TSNA species, 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N-nitrosonornicotine (NNN), have been shown to be strong carcinogens in numerous animal studies. We investigated the potential of molecular genetic strategies to lower nitrate levels in burley tobaccos by overexpressing genes encoding key enzymes of the nitrogen-assimilation pathway. Of the various constructs tested, only the expression of a constitutively active nitrate reductase (NR) …