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- Keyword
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- Ammi majus (3)
- A Reliable and High Yielding Method for Isolation of Genomic DNA from Ammi majus. (1)
- Abiotic (1)
- Agrobacterium tumefaciens (1)
- Berganpectane (1)
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- Biotic (1)
- DNA Isolation (1)
- Effect of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity and Other Factors in Successful Plant Transformation (1)
- Organogenesis (1)
- Organogenesis of Ammi majus via Callus Culture from Leaf. (1)
- Pearl millet (1)
- Phytotoxicity (1)
- Regeneration (1)
- Secondary metabolites (1)
- Xanthotoxin (1)
- Publication
Articles 1 - 4 of 4
Full-Text Articles in Life Sciences
Organogenesis Of Ammi Majus Via Callus Culture From Leaf., Sandip S. Magdum, Sanjeev Kumar
Organogenesis Of Ammi Majus Via Callus Culture From Leaf., Sandip S. Magdum, Sanjeev Kumar
Sandip S. Magdum
Ammi majus L. is an imperiled medicinal plant, which contains various medically important and uncommon extraordinary secondary metabolite compounds. An efficient organogenesis protocol was established for Ammi majus through in vitro process of leaf callusing, shooting and rooting by using different combination of auxins and cytokinins. In vitro cultured juvenile leaf explants were used directly for callus formation on MS supplemented with different concentration of IAA, Kn and CH. Maximum percentage response for callus formation was 97.7 after 5 weeks of incubation on MS supplemented with 2 mg/l IAA, 2 mg/l Kn and 1000 mg/l CH. About 81% of calluses …
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
Sandip S. Magdum
The developed protocol describes a cheaper, quicker and reliable method for the isolation of pure DNA from medicinal herbs, such as Ammi majus, which produces the secondary metabolites xanthotoxin and berganpectane having immense medicinal importance. Use of CTAB, liquid nitrogen and EDTA in different isolation protocols analyzed for A. majus, all were ended with polysaccharide and protein contamination with low purity of DNA (A260/280 = 1.3 – 1.6), revealed a need for method modification for the inexpensive and rapid isolation of pure DNA. Developed reliable and competent protocol isolated enough pure DNA (A260/280 = 1.81) without following time consuming lengthy …
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Effect Of Agrobacterium Induced Necrosis, Antibiotic Induced Phytotoxicity And Other Factors In Successful Plant Transformation., Sandip S. Magdum
Sandip S. Magdum
Agrobacterium tumefaciens infection and antibiotic wash are the critical steps of Agrobacterium mediated plant transformation procedure, most time responsible for lower transformation efficiency due to necrosis and phytotoxicity caused by biotic stress of Agrobacterium and abiotic stress by antibiotics respectively. Ammi majus Egyptian origin medicinal plant and Pearl millet cereal grain crop were studied for their stress responses to Agrobacterium mediated transformation (AMT). Agrobacterium strains LBA4404 (O.D.=0.6-0.8) and EHA105 (O.D.=0.2-0.4) were used for transformation experiments to infect calli of Ammi majus and embryogenic calli of Pearl millet respectively. Incase of antibiotic wash, Cefotaxime 500 mg L-1 was used for LBA4404 …
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Antioxidant Potential, Cytotoxic Activity And Total Phenolic Content Of Alpinia Pahangensis Rhizomes, Halijah Ibrahim, Phang C. W., Abd Malek S. N.
Halijah Ibrahim
BACKGROUND: Alpinia pahangensis, a wild ginger distributed in the lowlands of Pahang, Malaysia, is used by the locals to treat flatulence. In this study, the antioxidant and cytotoxic activities of the crude aqueous methanol and fractionated extracts of Alpinia pahangensis against five different cancer and one normal cell lines were investigated. The total phenolic content of each extract and its fractions were also quantified. This is the first report on the antioxidant and cytotoxic activities of Alpinia pahangensis extract. METHODS: In the current study, the crude methanol and fractionated extract of the rhizomes of Alpinia pahangensis were investigated for their …