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Identifying Brachypodium Distachyon Proteins Interacting With Histone Deacetylase Bdhd1, Alberto Giovanni Torrez

Electronic Thesis and Dissertation Repository

Current evidence has revealed the involvement of epigenetic mechanisms, including histone deacetylases (HDACs), in plant stress responses. In Arabidopsis thaliana, HDA19, belonging to the RPD3/HDA1 class, interacts with transcription factors to form repressor complexes. HDAC research mainly exists for dicotyledons, whereas research on monocotyledons is limited. Brachypodium distachyon is used as a model plant to investigate questions unique to monocot crops. BdHD1 is the closest homologous gene to HDA19 in B. distachyon. This study investigated potential protein-protein interactions between BdHD1 and each of BdMYB22, BdWRKY24, and BdHOS15. Protein interactions were investigated using yeast two-hybrid (Y2H) and bimolecular fluorescence …

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Evaluation Of The Water Stress-Inducible Promoter Wsi18 In The Model Monocot Brachypodium Distachyon, Patrick D. Langille

Electronic Thesis and Dissertation Repository

Water deficit-inducible promoters that function in multiple species are valuable components for engineering stress-tolerant crops. Wsi18 is a water deficit-inducible promoter native to Oryza sativa. In this study, Brachypodium distachyon (B. distachyon) was used to determine if Wsi18 retained its water deficit-inducible characteristics in another monocot. Transgenic B. distachyon plants, in which the Wsi18 promoter drove the expression of the uidA reporter gene, were developed and exposed to osmotic stress generated by mannitol, salt stress conditions, and the water deficit-signaling phytohormone abscisic acid (ABA). GUS histochemical assays demonstrated increased uidA expression in the leaves and stem of …