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Functional Analysis Of Two Brassinosteroid Responsive, Putative Calmodulin-Binding Proteins 60 (Cbp60s) In Arabidopsis Thaliana, Purvikalyan Pallegar
Functional Analysis Of Two Brassinosteroid Responsive, Putative Calmodulin-Binding Proteins 60 (Cbp60s) In Arabidopsis Thaliana, Purvikalyan Pallegar
Electronic Thesis and Dissertation Repository
Brassinosteroids (BRs) have remarkable ability to increase stress tolerance in plants. Investigations to understand the molecular mechanisms underlying BR-mediated stress tolerance resulted in identification of genes belonging to the family calmodulin binding protein X (CBPX). The present study was focused on studying the role of CBPX1 and CBPX2 in BR mediated stress tolerance and functional characterization using a reverse genetic approach. The upregulation of CBPX1 and CBPX2 by BR and stress noted in publicly available AtGenexpress datasets and by qRT-PCR analysis strongly suggests that these are BR responsive genes and functional analysis of T-DNA insertion mutants showed salt stress related …
Brassinosteroid-Mediated Stress Tolerance In Arabidopsis Thaliana, Tawhidur Rahman
Brassinosteroid-Mediated Stress Tolerance In Arabidopsis Thaliana, Tawhidur Rahman
Electronic Thesis and Dissertation Repository
Brassinosteroids (BRs) are a group of steroidal plant hormones that are essential for proper plant development and also promote stress tolerance. Without BRs, plants are dwarfs and infertile. To understand the molecular mechanisms underlying BR-mediated stress tolerance, global gene expression analysis of untreated and 24-epibrassinolide (EBR)-treated Arabidopsis thaliana seedlings under non-stress and heat stress (HS) conditions was carried out. Microarray data analysis indicated that stress-related genes were predominant within the EBR up-regulated gene data set. Furthermore, several of these genes were abscisic acid (ABA) and jasmonic acid (JA) related. Measurements of endogenous hormones showed significant increases in the levels ABA …