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Full-Text Articles in Life Sciences
Plant Mechanosensitive Ion Channels: An Ocean Of Possibilities, Debarati Basu, Elizabeth S. Haswell
Plant Mechanosensitive Ion Channels: An Ocean Of Possibilities, Debarati Basu, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Mechanosensitive ion channels, transmembrane proteins that directly couple mechanical stimuli to ion flux, serve to sense and respond to changes in membrane tension in all branches of life. In plants, mechanosensitive channels have been implicated in the perception of important mechanical stimuli such as osmotic pressure, touch, gravity, and pathogenic invasion. Indeed, three established families of plant mechanosensitive ion channels play roles in cell and organelle osmoregulation and root mechanosensing - and it is likely that many other channels and functions await discovery. Inspired by recent discoveries in bacterial and animal systems, we are beginning to establish the conserved and …
Life Behind The Wall: Sensing Mechanical Cues In Plants, Olivier Hamant, Elizabeth S. Haswell
Life Behind The Wall: Sensing Mechanical Cues In Plants, Olivier Hamant, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
There is increasing evidence that all cells sense mechanical forces in order to perform their functions. In animals, mechanotransduction has been studied during the establishment of cell polarity, fate, and division in single cells, and increasingly is studied in the context of a multicellular tissue. What about plant systems? Our goal in this review is to summarize what is known about the perception of mechanical cues in plants, and to provide a brief comparison with animals.
Mechanosensitive Channel Msl8 Regulates Osmotic Forces During Pollen Hydration And Germination, Eric S. Hamilton, Gregory S. Jensen, Grigory Maksaev, Andrew Katims, Ashley M. Sherp, Elizabeth S. Haswell
Mechanosensitive Channel Msl8 Regulates Osmotic Forces During Pollen Hydration And Germination, Eric S. Hamilton, Gregory S. Jensen, Grigory Maksaev, Andrew Katims, Ashley M. Sherp, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Pollen grains undergo dramatic changes in cellular water potential as they deliver the male germ line to female gametes, and it has been proposed that mechanosensitive ion channels may sense the resulting mechanical stress. Here, we identify and characterize MscS-like 8 (MSL8), a pollen-specific, membrane tension–gated ion channel required for pollen to survive the hypoosmotic shock of rehydration and for full male fertility. MSL8 negatively regulates pollen germination but is required for cellular integrity during germination and tube growth. MSL8 thus senses and responds to changes in membrane tension associated with pollen hydration and germination. These data further suggest that …
United In Diversity: Mechanosensitive Ion Channels In Plants, Eric S. Hamilton, Angela M. Schlegel, Elizabeth S. Haswell
United In Diversity: Mechanosensitive Ion Channels In Plants, Eric S. Hamilton, Angela M. Schlegel, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Mechanosensitive (MS) ion channels are a common mechanism for perceiving and responding to mechanical force. This class of mechanoreceptors is capable of transducing membrane tension directly into ion flux. In plant systems, MSion channels have been proposed to play a wide array of roles, from the perception of touch and gravity to the osmotic homeostasis of intracellular organelles. Three families of plant MS ion channels have been identified: the MscS-like (MSL), Mid1-complementing activity (MCA), and two-pore potassium (TPK) families. Channels from these families vary widely in structure and function, localize to multiple cellular compartments, and conduct chloride, calcium, and/or potassium …
Arabidopsis Msl10 Has A Regulated Cell Death Signaling Activity That Is Separable From Its Mechanosensitive Ion Channel Activity, Kira M. Veley, Grigory Maksaev, Elizabeth M. Frick, Emma January, Sarah C. Kloepper, Elizabeth S. Haswell
Arabidopsis Msl10 Has A Regulated Cell Death Signaling Activity That Is Separable From Its Mechanosensitive Ion Channel Activity, Kira M. Veley, Grigory Maksaev, Elizabeth M. Frick, Emma January, Sarah C. Kloepper, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
Members of the MscS superfamily of mechanosensitive ion channels function as osmotic safety valves, releasing osmolytes under increased membrane tension. MscS homologs exhibit diverse topology and domain structure, and it has been proposed that the more complex members of the family might have novel regulatory mechanisms or molecular functions. Here, we present a study of MscS-Like (MSL)10 from Arabidopsis thaliana that supports these ideas. High-level expression of MSL10-GFP in Arabidopsis induced small stature, hydrogen peroxide accumulation, ectopic cell death, and reactive oxygen species- and cell death-associated gene expression. Phosphomimetic mutations in the MSL10 N-terminal domain prevented these phenotypes. The phosphorylation …
Mscs-Like Mechanosensitive Channels In Plants And Microbes, Margaret E. Wilson, Grigory Maksaev, Elizabeth S. Haswell
Mscs-Like Mechanosensitive Channels In Plants And Microbes, Margaret E. Wilson, Grigory Maksaev, Elizabeth S. Haswell
Biology Faculty Publications & Presentations
The challenge of osmotic stress is something all living organisms must face as a result of environmental dynamics. Over the past three decades, innovative research and cooperation across disciplines have irrefutably established that cells utilize mechanically gated ion channels to release osmolytes and prevent cell lysis during hypoosmotic stress. Early electrophysiological analysis of the inner membrane of Escherichia coli identified the presence of three distinct mechanosensitive activities. The subsequent discoveries of the genes responsible for two of these activities, the mechanosensitive channels of large (MscL) and small (MscS) conductance, led to the identification of two diverse families of mechanosensitive channels. …