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Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells, Michael E. Urbanowski
Development Of A Cytosolic Ph Reporter For Tobacco By2 Cells, Michael E. Urbanowski
Masters Theses 1911 - February 2014
The regulation of pH is a critical homeostatic function of plant cells. In addition to acting as the primary cationic species responsible for energizing the plasma membrane, protons likely act as an important regulator and messenger. Despite this importance, few studies have thoroughly described cytosolic pH patterns as the plant cell progresses through the cell cycle. To investigate pH in plant cells, I chose Nicotiana tabacum (tobacco) Bright Yellow-2 (BY-2) cells as a model system. My research has two aims. First, I will measure and report the interphase cytosolic pH of BY-2 cells. Next, I will assay the cytosolic pH …