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A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
A Reliable And High Yielding Method For Isolation Of Genomic Dna From Ammi Majus., Sandip S. Magdum
Sandip S. Magdum
The developed protocol describes a cheaper, quicker and reliable method for the isolation of pure DNA from medicinal herbs, such as Ammi majus, which produces the secondary metabolites xanthotoxin and berganpectane having immense medicinal importance. Use of CTAB, liquid nitrogen and EDTA in different isolation protocols analyzed for A. majus, all were ended with polysaccharide and protein contamination with low purity of DNA (A260/280 = 1.3 – 1.6), revealed a need for method modification for the inexpensive and rapid isolation of pure DNA. Developed reliable and competent protocol isolated enough pure DNA (A260/280 = 1.81) without following time consuming lengthy …