Life Sciences Commons^™

Machine learning was applied to a challenging and biologically significant protein classification problem: the prediction of avonoid UGT acceptor regioselectivity from primary sequence. Novel indices characterizing graphical models of residues were proposed and found to be widely distributed among existing amino acid indices and to cluster residues appropriately. UGT subsequences biochemically linked to regioselectivity were modeled as sets of index sequences. Several learning techniques incorporating these UGT models were compared with classifications based on standard sequence alignment scores. These techniques included an application of time series distance functions to protein classification. Time series distances defined on the index sequences were …

This report analyzes the distribution of limonin within a leaf, within a branch, and between branches of an individual Citrus paradisi tree.  Limonin was evenly distributed within single leaves and the concentration in the leaves on a given branch was constant, regardless of branch age.  The total amount of limonin per leaf varied with leaf size.  Not all branches within the tree exhibited the same potential for limonin metabolism as reflected in their limonin and limonoate A-ring monolactone levels.  The limonin content showed increasing levels as the leaves bud and grow and then decreasing levels as they mature and turn …

An analysis of the limonin and naringin content of 6685 grapefruit juice samples (representing approximately 6% of all grapefruit harvested) collected from three Florida State Test Houses showed that there were statistically significant differences between the Test Houses.  There also was a statistically significant difference in the concentration of these compounds in the juice from the different cultivars of fruit sampled.  Average limonin and naringin concentrations in the juice remained fairly constant through the beginning of the season until a freeze occurred in early 1981.  Juice obtained from fruit harvested after this freeze contained decreasing amounts of limonin and increasing …

This report presents the results of a study conducted to determine the distribution of limonin within the fruit tissues of nine grapefruit cultivars.  It was found that the highest limonin levels within the fruit tissues were found in the cotyledon, followed by inner seed coat, outer seed coat, central pith, segment membrane, albedo and flavedo, and juice vesicles, in decreasing amounds.  The concentration of limonin in the tissues of the individual cultivars is also presented, and the variation in limonin content within a single tissue and within single fruit is discussed.  The limonin content of the fruit of the nine …