Life Sciences Commons^™

The Biology And Management Of Palmer Amaranth (Amaranthus Palmeri S. Wats) In Indiana, Douglas J. Spaunhorst

Open Access Dissertations

Palmer amaranth is a pernicious summer annual weed that has evolved resistance to six herbicide sites of action in the U.S. and threatens agriculture production. In 2011, Palmer amaranth was identified in flood plains in two southern Indiana counties (Posey and Vanderburgh). Determining if Palmer amaranth can survive and reproduce in northern Indiana may provide insight if this weed will be problematic for northern row crop producers. The objectives of our research were to identify fields containing Palmer amaranth and determine the distribution of herbicide resistance traits in Indiana; determine the influence of tillage frequency and tillage intervals on Palmer …

The Biology And Management Of Waterhemp In Indiana, Joseph M. Heneghan

Open Access Theses

Waterhemp is a dioecious weed species indigenous to the Midwestern United states yet it has only recently become problematic in agronomic crop production in Indiana. Waterhemp is a small-seeded broadleaf which has increased in prevalence in conjunction with an increase in conservation tillage practices. Waterhemp germinates and emerges from the top 3 cm of soil and is known to exhibit extended periods of continual emergence, longer than most other summer annual weed species that are typically present in agronomic production settings. As a C4 species, waterhemp then grows rapidly and is capable of producing thousands of seeds, while effectively competing …

Invasive Silver Carp (Hypophthalmichthys Moltrix) Protein Hydrolysates And Their Efficacy In Increasing Seedling Vigor And Reducing Seed Borne Pathogen Growth, Shanleigh Pauline Thomson

Open Access Theses

Silver carp are an invasive fish species introduced into the United States within the last forty years. Unable to be effectively eradicated or commercialized as a source of food, other methods must be developed to control and/or use these fish. Fish protein hydrolysates (FPH) are products derived from the proteins inherent within fish. Development of FPH from Silver carp for a variety of uses could aid in controlling their expanding population and provide organic produces with a seed treatment option which is not available to them currently.

In the first study, FPH were used as a seed vigor treatment for …

Using Energy-Efficient Technologies To Produce Bedding Plants And Microgreens In Protected And Controlled Environments, Joshua R. Gerovac

Open Access Theses

Gerovac, Joshua R. M.S., Purdue University, December 2014. Using Energy-Efficient Technologies to Produce Bedding Plants and Microgreens in Protected and Controlled Environments. Major Professor: Roberto G. Lopez. Production of bedding plants in commercial greenhouses (GHs) located in northern latitudes begins in late winter and continues through late spring when low outdoor temperatures generally necessitate active heating to maintain temperatures suitable for growth and development. Meanwhile, year-round production of microgreens using multi-layer systems requires sole-source (SS) photosynthetic lighting for production. Energy used to provide active heating in commercial GHs or SS lighting in multi-layer systems is second only to labor as …

Spatial And Temporal Distribution Of Fungicides Applied To Creeping Bentgrass, Kurt Ronald Hockemeyer

Open Access Theses

Turf managers often rely on fungicides to limit damage caused by root diseases. Since fungicides do not move basipetally, they are effective only when fungitoxic concentrations are delivered to the rhizosphere. This research focused on the distribution of modern fungicides in verdure, thatch, sand, and roots of creeping bentgrass (Agrostis stolonifera L. var. palustris (Huds.) Farw.) maintained as a putting green. Fungicides (azoxystrobin (methyl (E)-2-[2-[6-(2-cyanophenoxy)pyrimidin-4-yloxy]phenyl]-3-methoxyacrylate), propiconazole (1,2,4-Triazole, 1-((2-(2,4-dichlorophenyl)-4-propyl-1,3-dioxolan-2-yl)methyl), pyraclostrobin (carbamic acid, [2-[[[1-(4-chlorophenyl)-1H-pyrazol-3-yl]oxy]methyl]phenyl]methoxy-,methyl ester), and thiophanate-methyl (dimethyl 4,4'-o-phenylenebis[3-thioallophanate]) were applied to replicate field plots in a water volume of 815 L ha-1. Plots were sampled over time (0, 3, 7, 10, …

Genetic Engineering Of Black Cherry (Prunus Serotina) For Reproductive Sterility And Insect Pest Resistance, Ying Wang

Open Access Dissertations

Black cherry (Prunus serotina Ehrh.) is one of the most valuable hardwoods for high- end cabinetry, furniture, architectural millwork, paneling, and veneer. However, the damage caused by cambial-mining insect pests triggers gummosis in black cherry, a non-specific defense response in which resinous gum is deposited at the site of injury. The gum defects dramatically decrease the yield of high-quality black cherry lumber, and the value can be reduced by as much as 90%. The goal of this project was to optimize the transformation and in vitro rooting system, and to develop transgenic black cherry for reproductive sterility and insect pest …

Functional Characterization Of Metallothioneins In Arabidopsis And Barley, Nimnara Yookongkaew

Open Access Dissertations

Metalothioneins (MTs) are metal binding proteins that can bind metals such as Cu, Zn, and Cd but the functions of MTs in plants are largely unknown. To understand the function of MTs in Arabidopsis, T-DNA insertion mutants lacking 4 MT genes including MT1a, MT2a, MT2b and MT3 were developed. The quadruple mutant (mt1a/mt2a/mt2b/mt3) showed no visible phenotype under standard growth conditions. However, it accumulated higher Cu in leaves compared to wild type but lower Cu in seeds. Further analysis showed that triple mutants lacking both MT1a and MT2b, which are highly expressed in vascular tissues, had similar …

Developing White Ash With Resistance To The Emerald Ash Borer, Kaitlin Joy Palla

Open Access Theses

Hypocotyls isolated from mature embryos germinated on Murashige and Skoog (MS) medium supplemented with 22.2 µM 6-benzyladenine (BA) and 0.5 µM thidiazuron (TDZ) were transformed using Agrobacterium tumefaciens strain EHA105 harboring the pBI121-8D2 vector containing the neomycin phosphotransferase (nptII) gene and a full length, codon-optimized Bacillus thuringiensis Cry8Da protoxin gene (cry8D2). Explants were transformed in a bacterial suspension with 100 µM acetosyringone using 90 s sonication and 10 min vacuum-infiltration. Four independent lines of transformed shoots were selectively regenerated on MS medium with 22.2 µM BA, 0.5 µM TDZ, 50 mg L^-1 adenine sulfate, 10% …