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Transcriptional Responses Of Arabidopsis Thaliana Plants To As (V) Stress, Jason Abercrombie, Matthew Halfhill, Priya Ranjan, Murali Rao, Arnold Saxton, Joshua Yuan, Neal Stewart Dec 2015

Transcriptional Responses Of Arabidopsis Thaliana Plants To As (V) Stress, Jason Abercrombie, Matthew Halfhill, Priya Ranjan, Murali Rao, Arnold Saxton, Joshua Yuan, Neal Stewart

C. Neal Stewart Jr.

Background Arsenic is toxic to plants and a common environmental pollutant. There is a strong chemical similarity between arsenate [As (V)] and phosphate (Pi). Whole genome oligonucleotide microarrays were employed to investigate the transcriptional responses of Arabidopsis thaliana plants to As (V) stress. Results Antioxidant-related genes (i.e. coding for superoxide dismutases and peroxidases) play prominent roles in response to arsenate. The microarray experiment revealed induction of chloroplast Cu/Zn superoxide dismutase (SOD) (at2g28190), Cu/Zn SOD (at1g08830), as well as an SOD copper chaperone (at1g12520). On the other hand, Fe SODs were strongly repressed in response to As (V ...


Seorious Business: Structural Proteins In Sieve Tubes And Their Involvement In Sieve Element Occlusion [Review Article], Michael Knoblauch, Daniel R. Froelich, William F. Pickard, Winfried S. Peters Mar 2014

Seorious Business: Structural Proteins In Sieve Tubes And Their Involvement In Sieve Element Occlusion [Review Article], Michael Knoblauch, Daniel R. Froelich, William F. Pickard, Winfried S. Peters

Winfried S. Peters

The phloem provides a network of sieve tubes for long-distance translocation of photosynthates. For over a century, structural proteins in sieve tubes have presented a conundrum since they presumably increase the hydraulic resistance of the tubes while no potential function other than sieve tube or wound sealing in the case of injury has been suggested. Here we summarize and critically evaluate current speculations regarding the roles of these proteins. Our understanding suffers from the suggestive power of images; what looks like a sieve tube plug on micrographs may not actually impede translocation very much. Recent reports of an involvement of ...


A Guide To Southern Temperate Seagrasses, Kathryn Mcmahon, Michelle Waycott, Paul Lavery Jan 2014

A Guide To Southern Temperate Seagrasses, Kathryn Mcmahon, Michelle Waycott, Paul Lavery

Kathryn McMahon

No abstract provided.


Plants, Processes, Places: Sensory Intimacy And Poetic Enquiry, John Ryan Aug 2013

Plants, Processes, Places: Sensory Intimacy And Poetic Enquiry, John Ryan

John Ryan

As an arts-based research approach, poetic enquiry has been theorised and applied recently in the social sciences and in education. In this article, I extend its usage to eco-critical studies of Australian flora and fauna. The Southwest corner of Western Australia affords opportunities to deploy arts-based methodologies, including field poetry, for celebrating the natural heritage of a region of distinguished biodiversity. I suggest that lyric practices in places such as Lesueur National Park and Anstey-Keane Damplands in southern Perth can catalyse embodied engagements with flora. The outcome of these practices is the invocation of the multiple senses— including the proximities ...


Combined Morphological And Molecular Phylogeny Of The Clusioid Clade (Malpighiales) And The Placement Of The Ancient Rosid Macrofossil Paleoclusia, Brad Ruhfel, Peter Stevens, Charles Davis Dec 2012

Combined Morphological And Molecular Phylogeny Of The Clusioid Clade (Malpighiales) And The Placement Of The Ancient Rosid Macrofossil Paleoclusia, Brad Ruhfel, Peter Stevens, Charles Davis

Brad R. Ruhfel

Premise of research. The clusioid clade is a member of the large rosid order Malpighiales and contains∼1900 species in five families: Bonnetiaceae, Calophyllaceae, Clusiaceae sensu stricto (s.s.), Hypericaceae, and Podostemaceae. Despite recent efforts to clarify their phylogenetic relationships using molecular data, no such data are available for several critical taxa, including especially Hypericum ellipticifolium (previously recognized in Lianthus), Lebrunia, Neotatea, Thysanostemon, and the second-oldest rosid fossil (∼90 Ma), Paleoclusia chevalieri. Here, we (i) assess congruence between phylogenies inferred from morphological and molecular data, (ii) analyze morphological and molecular data simultaneously to place taxa lacking molecular data, and (iii ...


Resource-Use Strategies Of Native And Invasive Plants In Eastern North American Forests, J. Heberling, Jason Fridley Dec 2012

Resource-Use Strategies Of Native And Invasive Plants In Eastern North American Forests, J. Heberling, Jason Fridley

Mason Heberling

No abstract provided.


Plant Classification And Nomenclature In Ndumba, Papua New Guinea Highlands, Terence Hays Nov 2012

Plant Classification And Nomenclature In Ndumba, Papua New Guinea Highlands, Terence Hays

Terence Hays

Traditionally, the terms "ethnobotany" and "ethnozoology" have designated little more than the study of plant and animal utilization. In the past two decades, however, the ways in which the components of given biological environments are locally perceived and categorized have received increasing attention. Not only has the study of ethnobiological classification been recognized as essential to a wide variety of ethnographic concerns (cf. Frake 1962; Bulmer 1967), but the discovery of possible universals in folk classification systems promises to enrich our understanding of human cognitive processes as well (Berlin et al. 1973; Brown 1977).

The paucity of comprehensive studies of ...


The Effect Of Light Intensity On Seed Production And Quality In A Number Of Australian Wild Oat (Avena Fatua L.) Lines, Steve Adkins, Leisa Armstrong Feb 2012

The Effect Of Light Intensity On Seed Production And Quality In A Number Of Australian Wild Oat (Avena Fatua L.) Lines, Steve Adkins, Leisa Armstrong

Leisa Armstrong

Several aspects of the maternal environment (e.g. air temperature, soil moisture, soil nutritional status and photoperiod) are known to influence seed production and quality in a range of plant species. However, little is known about the effect of other environmental factors, such as the light intensity perceived by the developing plant, on these seed characters. This is particularly important when elucidating the persistence mechanisms of annual weeds that may produce seeds under a crop canopy. Therefore, the aim of the present study was to determine the effect of light intensity on the reproductive characteristics of a number of wild ...


Scale Dependence Of Vegetation–Environment Relationships: A Meta-Analysis Of Multivariate Data, Andrew Siefert, Catherine Ravenscroft, David Althoff, Juan Alvarez-Yépiz, Benjamin Carter, Kelsey Glennon, J. Heberling, In Su Jo, Alyssa Pontes, Amy Sauer, Adam Willis, Jason Fridley Dec 2011

Scale Dependence Of Vegetation–Environment Relationships: A Meta-Analysis Of Multivariate Data, Andrew Siefert, Catherine Ravenscroft, David Althoff, Juan Alvarez-Yépiz, Benjamin Carter, Kelsey Glennon, J. Heberling, In Su Jo, Alyssa Pontes, Amy Sauer, Adam Willis, Jason Fridley

Mason Heberling

No abstract provided.


Ethylene Receptor Ethylene Receptor1 Domain Requirements For Ethylene Responses In Arabidopsis Seedlings, Heejung Kim, Elizabeth Helmbrecht, M. Stalins, Christina Schmitt, Nesha Patel, Chi-Kuang Wen, Wuyi Wang, Brad Binder Apr 2011

Ethylene Receptor Ethylene Receptor1 Domain Requirements For Ethylene Responses In Arabidopsis Seedlings, Heejung Kim, Elizabeth Helmbrecht, M. Stalins, Christina Schmitt, Nesha Patel, Chi-Kuang Wen, Wuyi Wang, Brad Binder

Brad M. Binder

Ethylene influences many processes in Arabidopsis (Arabidopsis thaliana) through the action of five receptor isoforms. We used high-resolution, time-lapse imaging of dark-grown Arabidopsis seedlings to better understand the roles of each isoform in the regulation of growth in air, ethylene-stimulated nutations, and growth recovery after ethylene removal. We found that ETHYLENE RECEPTOR1 (ETR1) is both necessary and sufficient for nutations. Transgene constructs in which the ETR1 promoter was used to drive expression of cDNAs for each of the five receptor isoforms were transferred into etr1-6;etr2-3;ein4-4 triple loss-of-function mutants that have constitutive growth inhibition in air, fail to nutate ...


Tolerance Of Two Invasive Thistles To Repeated Disturbance, Rui Zhang, Mason Heberling, Emily Haner, Katriona Shea Dec 2010

Tolerance Of Two Invasive Thistles To Repeated Disturbance, Rui Zhang, Mason Heberling, Emily Haner, Katriona Shea

Mason Heberling

No abstract provided.


Proteomic Responses In Arabidopsis Thaliana Seedlings Treated With Ethylene, Ruiqiang Chen, Brad Binder, Wesley Garrett, Mark Tucker, Caren Chang, Bret Cooper Dec 2010

Proteomic Responses In Arabidopsis Thaliana Seedlings Treated With Ethylene, Ruiqiang Chen, Brad Binder, Wesley Garrett, Mark Tucker, Caren Chang, Bret Cooper

Brad M. Binder

Ethylene (ET) is a volatile hormone that modulates fruit ripening, plant growth, development and stress responses. Key components of the ET-signaling pathway identified by genetic dissection in Arabidopsis thaliana include five ET receptors, the negative regulator CTR1 and the positive regulator EIN2, all of which localize to the endoplasmic reticulum. Mechanisms of signaling among these proteins are still unresolved and targets of ET responses are not fully known. So, we used mass spectrometry to identify proteins in microsomal membrane preparations from etiolated A. thaliana seedlings maintained in ambient air or treated with ET for 3 h. We compared 3814 proteins ...


Ethylene Receptors Function As Components Of High-Molecular-Mass Protein Complexes In Arabidopsis, Brad Binder, Yi-Feng Chen, Zhiyong Gao, Robert Kerris, Wuyi Wang, G. Schaller Dec 2009

Ethylene Receptors Function As Components Of High-Molecular-Mass Protein Complexes In Arabidopsis, Brad Binder, Yi-Feng Chen, Zhiyong Gao, Robert Kerris, Wuyi Wang, G. Schaller

Brad M. Binder

Background The gaseous plant hormone ethylene is perceived in Arabidopsis thaliana by a five-member receptor family composed of ETR1, ERS1, ETR2, ERS2, and EIN4. Methodology/Principal Findings Gel-filtration analysis of ethylene receptors solubilized from Arabidopsis membranes demonstrates that the receptors exist as components of high-molecular-mass protein complexes. The ERS1 protein complex exhibits an ethylene-induced change in size consistent with ligand-mediated nucleation of protein-protein interactions. Deletion analysis supports the participation of multiple domains from ETR1 in formation of the protein complex, and also demonstrates that targeting to and retention of ETR1 at the endoplasmic reticulum only requires the first 147 amino ...


Ethylene-Dependent And -Independent Regulation Of Abscission, Brad Binder, Sara Patterson Jan 2009

Ethylene-Dependent And -Independent Regulation Of Abscission, Brad Binder, Sara Patterson

Brad M. Binder

Purpose of review: This review focuses on recent research on the control of abscission, highlighting ethylene dependent and independent mechanisms. Findings: Abscission is the loss of organs such as fruit, leaves and floral organs. Ethylene has long been recognised as one of the most important factors controlling abscission. However, abscission is also controlled by a complex network of factors not related to ethylene, including auxin, jasmonic acid and peptides. Recent increased understandings of the nature of both ethylene-dependent and independent abscission control are expected to suggest more refined methods for abscission control in horticultural plants. Directions for future research: A ...


Recent Advances In The Study Of Gynodioecy: The Interface Of Theory And Empiricism, Maia Bailey, David Mccauley Dec 2008

Recent Advances In The Study Of Gynodioecy: The Interface Of Theory And Empiricism, Maia Bailey, David Mccauley

Maia F. Bailey

No abstract provided.


The Btb Ubiqutin Ligases Eto1, Eol1 And Eol2 Act Collectively To Regulate Ethylene Biosynthesis In Arabidopsis By Controlling Type-2 Acc Synthase Levels, Brad Binder, Matthew Christians, Derek Gingerich, Moureen Hansen, Joseph Kieber, Richard Vierstra Dec 2008

The Btb Ubiqutin Ligases Eto1, Eol1 And Eol2 Act Collectively To Regulate Ethylene Biosynthesis In Arabidopsis By Controlling Type-2 Acc Synthase Levels, Brad Binder, Matthew Christians, Derek Gingerich, Moureen Hansen, Joseph Kieber, Richard Vierstra

Brad M. Binder

Ethylene biosynthesis is directed by a family of 1-aminocyclopropane-1-carboxylic acid (ACC) synthases (ACS) that convert S-adenosyl-l-methionine to the immediate precursor ACC. Members of the type-2 ACS subfamily are strongly regulated by proteolysis with various signals stabilizing the proteins to increase ethylene production. In Arabidopsis, this turnover is mediated by the ubiquitin/26 S proteasome system, using a broad complex/tramtrack/bric-a-brac (BTB) E3 assembled with the ETHYLENE OVERPRODUCER 1 (ETO1) BTB protein for target recognition. Here, we show that two Arabidopsis BTB proteins closely related to ETO1, designated ETO1-like (EOL1) and EOL2, also negatively regulate ethylene synthesis via their ability ...


Heteromeric Interactions Among Ethylene Receptors Mediate Signaling In Arabidopsis, Brad Binder, Zhiyong Gao, Chi-Kuang Wen, Yi-Feng Chen, Jianhong Chang, Yi-Hsuan Chiang, Robert Kerris, Caren Chang, G. Schaller Jul 2008

Heteromeric Interactions Among Ethylene Receptors Mediate Signaling In Arabidopsis, Brad Binder, Zhiyong Gao, Chi-Kuang Wen, Yi-Feng Chen, Jianhong Chang, Yi-Hsuan Chiang, Robert Kerris, Caren Chang, G. Schaller

Brad M. Binder

The gaseous hormone ethylene is perceived in Arabidopsis by a five member receptor family that consists of the subfamily 1 receptors ETR1 and ERS1 and the subfamily 2 receptors ETR2, ERS2, and EIN4. Previous work has demonstrated that the basic functional unit for the ethylene receptor, ETR1, is a disulfide-linked homodimer. We demonstrate here that ethylene receptors isolated from Arabidopsis also interact with each other through noncovalent interactions. Evidence that ETR1 associates with other ethylene receptors was obtained by co-purification of ETR1 with tagged versions of ERS1, ETR2, ERS2, and EIN4 from Arabidopsis membrane extracts. ETR1 preferentially associated with the ...


The Ethylene Receptors: Complex Perception For A Simple Gas, Brad Binder Jun 2008

The Ethylene Receptors: Complex Perception For A Simple Gas, Brad Binder

Brad M. Binder

Ethylene is a hormone that regulates a number of physiological and developmental events in plants. This simple olefin is perceived by a family of receptors that have homology to bacterial two-component receptors. Although the ethylene receptors have been the focus of much study over the past several decades, many questions remain concerning their structure, function, and regulation. This review provides an overview on what is known about the ethylene receptors, summarizes recent research on the receptors, and presents models for receptor function and output.


Ethylene Receptor Antagonists: Strained Alkenes Are Necessary But Not Sufficient, Brad Binder, Michael Pirrung, Anthony Bleecker, Yoshihisa Inoue, Fernando Rodríguez, Norimitsu Sugawara, Takehiko Wada, Yunfan Zou Mar 2008

Ethylene Receptor Antagonists: Strained Alkenes Are Necessary But Not Sufficient, Brad Binder, Michael Pirrung, Anthony Bleecker, Yoshihisa Inoue, Fernando Rodríguez, Norimitsu Sugawara, Takehiko Wada, Yunfan Zou

Brad M. Binder

Plants use ethylene as a hormone to control many physiological processes. Ethylene perception involves its binding to an unusual copper-containing, membrane-bound receptor. Inhibitors of ethylene action are valuable to study signaling and may have practical use in horticulture. Past investigation of alkene ligands for this receptor has identified strain as the key factor in antagonism of ethylene binding and action, consistent with known trends in metal-alkene complex stability. However, in this work, this principle could not be extended to other alkenes, prompting development of the proposal that a ring-opening reaction accounts for the unusual potency of cyclopropene ethylene antagonists. Another ...


The Effects Of Group 11 Transition Metals, Including Gold, On Ethylene Binding To The Etr1 Receptor And Growth Of Arabidopsis Thaliana, Brad Binder, Fernando Rodriguez, Anthony Bleecker, Sara Patterson Sep 2007

The Effects Of Group 11 Transition Metals, Including Gold, On Ethylene Binding To The Etr1 Receptor And Growth Of Arabidopsis Thaliana, Brad Binder, Fernando Rodriguez, Anthony Bleecker, Sara Patterson

Brad M. Binder

It has been previously shown that Cu(I) and the ethylene response antagonist, Ag(I), support ethylene binding to exogenously expressed ETR1 ethylene receptors. Both are Group 11 transition metals that also include gold. We compared the effects of gold ions with those of Cu(I) and Ag(I) on ethylene binding in exogenously expressed ETR1 receptors and on ethylene growth responses in etiolated Arabidopsis seedlings. We find that gold ions also support ethylene binding but, unlike Ag(I), do not block ethylene action on plants. Instead, like Cu(I), gold ions affect seedlings independently of ethylene signaling.


The Arabidopsis Ein3 Binding F-Box Proteins Ebf1 And Ebf2 Have Distinct But Overlapping Roles In Ethylene Signaling, Brad Binder, Joseph Walker, Jennifer Gagne, Thomas Emborg, Georg Hemmann, Anthony Bleecker, Richard Vierstra Jan 2007

The Arabidopsis Ein3 Binding F-Box Proteins Ebf1 And Ebf2 Have Distinct But Overlapping Roles In Ethylene Signaling, Brad Binder, Joseph Walker, Jennifer Gagne, Thomas Emborg, Georg Hemmann, Anthony Bleecker, Richard Vierstra

Brad M. Binder

Ethylene signaling in Arabidopsis thaliana converges on the ETHYLENE-INSENSITIVE3 (EIN3)/EIN3-Like (EIL) transcription factors to induce various responses. EIN3 BINDING F-BOX1 (EBF1) and EBF2 were recently shown to function in ethylene perception by regulating EIN3/EIL turnover. In the absence of ethylene, EIN3 and possibly other EIL proteins are targeted for ubiquitination and subsequent degradation by Cullin 1–based E3 complexes containing EBF1 and 2. Ethylene appears to block this ubiquitination, allowing EIN3/EIL levels to rise and mediate ethylene signaling. Through analysis of mutant combinations affecting accumulation of EBF1, EBF2, EIN3, and EIL1, we show that EIN3 and EIL1 ...


Rapid Kinetic Analysis Of Ethylene Growth Responses In Seedlings: New Insights Into Ethylene Signal Transduction, Brad Binder Dec 2006

Rapid Kinetic Analysis Of Ethylene Growth Responses In Seedlings: New Insights Into Ethylene Signal Transduction, Brad Binder

Brad M. Binder

Ethylene is a phytohormone that influences diverse processes in plants. Ethylene causes various changes in etiolated seedlings that differ between species and include reduced growth of shoots and roots, increased diameter of shoots, agravitropic growth, initiation of root hairs, and increased curvature of the apical hook. The inhibition of growth in etiolated seedlings has become widely used to screen for and identify mutants. This approach has led to an increased understanding of ethylene signaling. Most studies use end-point analysis after several days of exposure to assess the effects of ethylene. Recently, the use of time-lapse imaging has re-emerged as an ...


Inheritance Of Chloroplast Dna Is Not Strictly Maternal In Silene Vulgaris (Caryophyllaceae): Evidence From Experimental Crosses And Natural Populations, David Mccauley, Maia Bailey, Allyson Sundby, Mark Welch Dec 2006

Inheritance Of Chloroplast Dna Is Not Strictly Maternal In Silene Vulgaris (Caryophyllaceae): Evidence From Experimental Crosses And Natural Populations, David Mccauley, Maia Bailey, Allyson Sundby, Mark Welch

Maia F. Bailey

No abstract provided.


Sex-Ratio Evolution In Nuclear-Cytoplasmic Gynodioecy When Restoration Is A Threshold Trait, Maia Bailey, Lynda Delph Dec 2006

Sex-Ratio Evolution In Nuclear-Cytoplasmic Gynodioecy When Restoration Is A Threshold Trait, Maia Bailey, Lynda Delph

Maia F. Bailey

No abstract provided.


Merging Theory And Mechanism In Studies Of Gynodioecy, Lynda Delph, Pascal Touzet, Maia Bailey Dec 2006

Merging Theory And Mechanism In Studies Of Gynodioecy, Lynda Delph, Pascal Touzet, Maia Bailey

Maia F. Bailey

No abstract provided.


The Exoribonuclease Xrn4 Is A Component Of The Ethylene Response Pathway In Arabidopsis, Brad Binder, Thomas Potuschak, Amérin Vansiri, Esther Lechner, Richard Vierstra, Pascal Genschik Oct 2006

The Exoribonuclease Xrn4 Is A Component Of The Ethylene Response Pathway In Arabidopsis, Brad Binder, Thomas Potuschak, Amérin Vansiri, Esther Lechner, Richard Vierstra, Pascal Genschik

Brad M. Binder

EXORIBONUCLEASE4 (XRN4), the Arabidopsis thaliana homolog of yeast XRN1, is involved in the degradation of several unstable mRNAs. Although a role for XRN4 in RNA silencing of certain transgenes has been reported, xrn4 mutant plants were found to lack any apparent visible phenotype. Here, we show that XRN4 is allelic to the unidentified components of the ethylene response pathway ETHYLENE-INSENSITIVE5/ACC-INSENSITIVE1 (EIN5/AIN1) and EIN7. xrn4 mutant seedlings are ethylene-insensitive as a consequence of the upregulation of EIN3 BINDING F-BOX PROTEIN1 (EBF1) and EBF2 mRNA levels, which encode related F-box proteins involved in the turnover of EIN3 protein, a crucial ...


Ethylene-Stimulated Nutations Do Not Require Etr1 Receptor Histidine Kinase Activity, Brad Binder Oct 2006

Ethylene-Stimulated Nutations Do Not Require Etr1 Receptor Histidine Kinase Activity, Brad Binder

Brad M. Binder

Ethylene influences the growth and development of plants through the action of receptors that have homology to bacterial two-component receptors. In bacteria these receptors function via autophosphorylation of a His residue in the kinase domain followed by phosphotransfer to a conserved Asp residue in a response regulator protein. In Arabidopsis, two of the five receptor isoforms are capable of His kinase activity. However, the role of His kinase activity and phosphotransfer is unclear in ethylene signaling. A previous study showed that ethylene stimulates nutations of the hypocotyl in etiolated Arabidopsis seedlings that are dependent on the ETR1 receptor isoform. The ...


Identification Of Important Regions For Ethylene Binding And Signaling In The Transmembrane Domain Of The Etr1 Ethylene Receptor Of Arabidopsis, Brad Binder, Wuyi Wang, Jeff Esch, Shin-Han Shiu, Hasi Agula, Caren Chang, Sara Patterson, Anthony Bleecker Dec 2005

Identification Of Important Regions For Ethylene Binding And Signaling In The Transmembrane Domain Of The Etr1 Ethylene Receptor Of Arabidopsis, Brad Binder, Wuyi Wang, Jeff Esch, Shin-Han Shiu, Hasi Agula, Caren Chang, Sara Patterson, Anthony Bleecker

Brad M. Binder

The ethylene binding domain (EBD) of the Arabidopsis thaliana ETR1 receptor is modeled as three membrane-spanning helices. We surveyed ethylene binding activity in different kingdoms and performed a bioinformatic analysis of the EBD. Ethylene binding is confined to land plants, Chara, and a group of cyanobacteria but is largely absent in other organisms, consistent with our finding that EBD-like sequences are overrepresented among plant and cyanobacterial species. We made amino acid substitutions in 37 partially or completely conserved residues of the EBD and assayed their effects on ethylene binding and signaling. Mutations primarily in residues in Helices I and II ...


Ethylene-Binding Activity, Gene-Expression Levels, And Receptor-System Output For Ethylene-Receptor Family Members From Arabidopsis And Tomato, Brad Binder, Ronan O'Malley, Fernando Rodriguez, Jeffrey Esch, Philip O'Donnell, Harry Klee, Anthony Bleecker Feb 2005

Ethylene-Binding Activity, Gene-Expression Levels, And Receptor-System Output For Ethylene-Receptor Family Members From Arabidopsis And Tomato, Brad Binder, Ronan O'Malley, Fernando Rodriguez, Jeffrey Esch, Philip O'Donnell, Harry Klee, Anthony Bleecker

Brad M. Binder

Ethylene signaling in plants is mediated by a family of ethylene receptors related to bacterial two-component regulators. Expression in yeast of ethylene-binding domains from the five receptor isoforms from Arabidopsis thaliana and five-receptor isoforms from tomato confirmed that all members of the family are capable of high-affinity ethylene-binding activity. All receptor isoforms displayed a similar level of ethylene binding on a per unit protein basis, while members of both subfamily I and subfamily II from Arabidopsis showed similar slow-release kinetics for ethylene. Quantification of receptor-isoform mRNA levels in receptor-deficient Arabidopsis lines indicated a direct correlation between total message level and ...


Evidence For Paternal Transmission And Heteroplasmy In The Mitochondrial Genome Of Silene Vulgaris, A Gynodioecious Plant, David Mccauley, Maia Bailey, Natasha Sherman, M. Darnell Dec 2004

Evidence For Paternal Transmission And Heteroplasmy In The Mitochondrial Genome Of Silene Vulgaris, A Gynodioecious Plant, David Mccauley, Maia Bailey, Natasha Sherman, M. Darnell

Maia F. Bailey

No abstract provided.