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Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
Effect Of Endothelial Progenitor Cell-Derived Exosomes On High Glucose And Hypoxia/ Reoxygenation-Induced Injury Of Astrocytes, Manasi Suchit Halurkar
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In this study, we tested the protective effects of EPC-EXs (endothelial progenitor cell derived exosomes) and miR-126 EPC-EXs (microRNA-126 EPC-EXs) on the astrocytes injured by HG (high glucose) plus H/R (hypoxia/reoxygenation) model. At first, we determined the concentration and time dependent uptake of EPC-EXs by astrocytes. It was also found that the EPC-EXs were uptaken via macropinocytosis, caveolin-dependent and clathrin-mediated pathways in astrocytes. Furthermore, the astrocyte cell line was injured through the HG + H/R model. EXs, isolated by ultracentrifugation from the EPC culture supernatant were co-incubated with the injured cells. It was found that EPC-EXs and miR-126 EPC-EXs decrease …