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Fine-Grained Bacterial Compositional Analsysis Of The Port Everglades Inlet (Broward County, Fl) Microbiome Using High Throughput Dna Sequencing, Lauren M. O'Connell
Fine-Grained Bacterial Compositional Analsysis Of The Port Everglades Inlet (Broward County, Fl) Microbiome Using High Throughput Dna Sequencing, Lauren M. O'Connell
HCNSO Student Theses and Dissertations
Port Everglades Inlet is one of the busiest ports in the country and is a point source of pollution to surrounding beaches and offshore corals from heavy boat traffic and urban runoff. Understanding fluctuations of bacterioplankton communities in major port inlets is important due to their impacts on surrounding marine environments. To understand annual microbial fluctuations, the 16s rRNA V4 hypervariable region was sequenced using Illumina high-throughput DNA sequencing technology. Surface samples were taken weekly for one year to generate baseline fluctuations in the microbial community. Total reads of 1.4 million were generated with a final count of 16,384 Operational …