Life Sciences Commons^™

Bacteriophage Populations In Wastewater Effluent, Guy William Lawrence

Masters Theses

Effluent samples were taken at various stages of sewage treatment at a local wastewater treatment plant. Primary, secondary, tertiary and post-chlorinated effluents were sampled. The agar overlay method was used to determine the numbers of plaque-forming bacteriophage particles on thirty-six species of enteric bacteria from the Eastern Illinois University stock culture collection. Twenty-three pure culture phage isolates were prepared from selected plaques and their host range specificity was determined. For every host organism tested, a fifty percent or greater reduction of bacteriophage was evident through the sewage treatment process. Results obtained from investigation of host range specificity indicate that potential …

Effects Of Polychlorinated Biphenyl Compounds And Proposed Pcb-Replacement Products On Embryo-Larval Stages Of Fish And Amphibians, Wesley J. Birge, Jeffrey A. Black, Albert G. Westerman

KWRRI Research Reports

Static renewal bioassays were performed with four polychlorinated biphenyls, including Capacitor 21 and Aroclors 1016, 1242, and 1254. Each compound was used to treat embryo-larval stages of the rainbow trout (Salmo gairdneri), channel catfish (Ictalurus punctatus), goldfish (Carassius auratus), redear sunfish (Lepomis microlophus), leopard frog (Rana pipiens), Fowler's toad (Bufo fowleri), and American toad (Bufo americanus). Developmental stages of the trout were the most sensitive, with LC₅₀ 's at 4 days posthatching of 0.3, 1.0, 1.1, and 1.6 μg/l for Aroclors 1254, 1242, 1016, and …

Purification Of The Protein Crystal From Bacillus Thuringiensis By Zonal Gradient Centrifugation, Kenneth W. Nickerson, Barabara J. Ang

Papers in Microbiology

A method is described for the large-scale purification of the Bacillus thuringiensis protein crystal by zonal gradient centrifugation. NaBr gradients are employed in a Beckman J21-B centrifuge equipped with a JCF-Z rotor.

Identification Of A Protein Linked To Nascent Poliovirus Rna And To The Polyuridylic Acid Of Negative-Strand Rna, Ralf F. Pettersson, Victor R. Ambros, David Baltimore

Victor R. Ambros

A protein similar to that previously demonstrated on poliovirus RNA and replicative intermediate RNA (VPg) was found on all sizes of nascent viral RNA molecules and on the polyuridylic acid isolated from negative-strand RNA. 32P-labeled nascent chains were released from their template RNA and fractionated by exclusion chromatography on agarose. Fingerprint analysis using two-dimensional polyacrylamide gels of RNase T1 oligonucleotides derived from nascent chains of different lengths showed that a size fractionation of nascent chains was achieved. VPg was recovered from nascent chains varying in length from 7,500 nucleotides (full-sized RNA) to about 500 nucleotides. No other type of 5' …

Effects Of Trypanosoma Musculi Kendall, 1906 Immune Serum On The Reproduction Of Trichinella Spiralis (Owen, 1835) Railliet, 1896 In Mice, Samuel L. Chafin

Loma Linda University Electronic Theses, Dissertations & Projects

The possible effects of ablastin, a reproduction-inhibiting antibody which is produced during the infection of the blood parasite, Trypanosoma musculi in the mouse, were investigated by fractionating the parasite into a supernatant fraction (designated as Group A test mice) and a pellet fraction (Group B test mice). These groups A and B mice were injected with the respective fractions, plus Freund’s Complete Adjuvant (FCA). Control mice were injected with FCA only. Sera from these three groups of mice were removed after the Group A and Group B mice were shown to be negative to T. musculi challenge, and Group A …

Effect Of Time And Diet On The Immune Status Of Mice Challenged With Herpes-Transformed Cells, Daila S. Gridley

Loma Linda University Electronic Theses, Dissertations & Projects

Human Herpes simplex virus type 2 (HSV 2)-transformed Balb/c mouse cells (H238 cells) are malignant in Balb/c mice. The immune stratus of mice bearing H238 cell-induced tumors was studied and changes in immune response with time and with altered diet of the mice were measured.

In the experiments dealing with the status of immunity with time after tumor cell injection, mice were fed Purina Laboratory Chow (P.L.C.), injected with H238 cells, and sacrificed three to eight weeks post-injection. In the diet experiments, groups of mice were fed either P.L.C. or one of six equicaloric diets, consisting of low or high …

Non-Parental-Type Recombinants In Crosses Between Different Restriction-Modification Systems In Salmonella, Mary Ball

Loma Linda University Electronic Theses, Dissertations & Projects

Many new restriction-modification (R-M) system which are closely linked to serB and allelic to the hsd_SB system of S. typimurium occur in different Salmonella serotypes. In PI transduction studies with these systems, recombinants were isolated which fell into one of three classes with reference to their specific restriction phenotype. Recombinants that restricted like the donor, recombinants that restricted like the recipient and recombinants that had lost the ability to restrict. This latter group having lost the recipient SB restriction without gaining the specific donor restriction and here called "zero recombinants,” were of special interest and were investigated.

Zero recombinants …

Reactivity Of Antibodies Versus Streptococcal Carbohydrate And Protein Antigens And Human Glomerular Basement Membrane Carbohydrate And Protein Antigens In Mammalian Tissues, Kathleen A. Chase

Master's Theses

No abstract provided.

Characterization Of The Nonfermentative Gram Negative Rods Of Group Iik Utilizing Biochemical, Serological, And Antibiotic Susceptibility Tests, Donna S. Granstrom

Master's Theses

No abstract provided.

Characterization Of Pseudomonas Cepacia Using Biochemical, Antibiotic Susceptibility And Serological Tests, Beatrice Grazina Miceika

Master's Theses

No abstract provided.

Cytology And Physiology Of Arthrospore Formation In The Dermatophyte Trichophyton Mentagrophytes, Ruth Gail Emyanitoff

Dissertations

No abstract provided.

In Vivo And In Vitro Interactions Between Bacteroides Fragilis And Escherichia Coli: Their Role In Intra-Abdominal Abscess Formation, James Carl Hagen

Dissertations

No abstract provided.

Bvr-1, A Restriction Locus Of A Type C Rna Virus In The Feline Cellular Genome: Pleiotropic Restriction Of Endogenous Balb Virus In Cat X Mouse Somatic Cell Hybrids, Stephen J. O'Brien, Janice M. Simonson

Biology Faculty Articles

Bvr-1 is a dominant X-linked feline gene which restricts the replication of B-tropic murineleukemia virus (B-MuLV) in somatic cell hybrids between murine BALB/c-RAG cells and FL-74 feline cells. Since the hybrids were originally derived by the hypoxanthine aminopterin thymidine selection scheme, counter selection experiments on 6-thioguanine result in preferential survival of hybrid cells which have spontaneously lost the feline X-chromosome on which is located the structural gene for hypoxanthine guanine phosphoribosyl transferase (IMP: pyrophosphate phosphoribosyl transferase, E.C. 2.4.2.8) and Bvr-1. Back selected Bvr-1^- cells express high parental levels of B-MuLV. Bvr-1^- effectively restricts the IdU-mediated induction of …

Listeria Monocytogenes In The Environment Of Livestock In South Dakota, James W. Neiles

Electronic Theses and Dissertations

The purpose of this investigation is to study the incidence of Listeria monocytogenes in the environment of livestock in South Dakota. The data accumulated from these studies will provide information as to its natural habitat, mode of transmission and factors responsible for its incidence.

Partial Characterization And Comparison Of Rhizobia From Astragalus Flexuosus Glycyrrhiza Lepidota, And Trifolium Spp., Elmer L. Eide

Electronic Theses and Dissertations

Biological nitrogen fixation, a means of alleviating our increasing dependence on synthesized nitrogen fertilizers, is being studied with renewed interest. Production of nitrogen fertilizer requires large amounts of natural gas and other non-renewable energy sources, while the energy for biological nitrogen fixation is derived either directly or indirectly from photosynthesis. With only limited supplies of natural gas and an increasing population, and thus increasing demand for food, the advantages and even necessity of understanding and enhancing biological nitrogen is obvious. The nitrogenase enzyme complex which catalyzes the reduction of dinitrogen to ammonia is possessed by a limited number of species …

Nonsporing Anaerobic Bacteria In Foods, Sylvia M. Huppler

Electronic Theses and Dissertations

Anaerobic non spore forming bacteria are not detected in routine food examinations, and their occurrences in foods has seldom been reported in the literature. The purpose of this study is to determine the occurrence of non-spore forming anaerobes, the identification of the isolates found, and the possible growth and multiplication in foods which might contribute to spoilage or food illness.

Partial Purification Of Heat Stable Enterotoxin Produced By Escherichia Coli Isolated From A Case Of Porcine Colibacillosis, Lee Ann Zochert Swanson

Electronic Theses and Dissertations

Colibacillosis (CB) in livestock is an economically important disease caused by the bacterium Escherichia coli. It is estimated that nearly one third of all baby pigs born in South Dakota die prior to weaning, and one fourth of these deaths is believed to be caused by CB (62). Similarly, it has been stated that 6 to 20% of calves born alive die from this disease (3). The term colibacillosis encompasses a group of diseases caused by E. coli in neonatal and weaned animals and humans (3). The several different types of CB are due to the differences in tissue localization …

Methodology Development For The Study Of The Anaerobic Bacterial Flora Of Bovine Feces, Donald G. Kirsch

Electronic Theses and Dissertations

The intestinal bacterial flora of an animal has a considerable influence on the physiology of that animal. Some bacteria of the intestine are pathogenic and can initiate infections if they gain access to a susceptible individual. Some of the intestinal bacteria can produce potent carcinogens in vitro and can presumably do this also in vivo. Food passing through the alimentary tract of an animal is also chemically modified through the enzymatic action of the bacterial flora. The animal is also protected from some enteric infections through the antagonistic and competitive actions of the established organisms of the normal bacterial flora. …

Rotaviral Enteritis In Gnotobiotic Pigs: A Scanning Electron Microscopic Study, Marcus W. Johnshoy

Electronic Theses and Dissertations

The objectives of this study were: 1. To experimentally reproduce rotaviral diarrhea in gnotobiotic pigs. 2. To determine the appearance of the normal intestine by scanning electron and light microscopy. 3. To compare by scanning electron and light microscopy the appearance of segments from different areas of intestine and to determine the development, location, and severity of rotaviral lesions in gnotobiotic pigs.

The Effects Of Residual Chlorine On Selected Microflora Of Kickapoo And Cassell Creek, James A. Mcgaughey

Masters Theses

The survival of fecal coliforms, fecal streptococci, and heterotrophs after exposure to chlorine were compared in receiving waters of the Mattoon and Charleston, Illinois waste treatment facilities employing membrane filter techniques and Standard Plate Counts. Water samples were taken from receiving waters upstream from the treatment plant, from secondary effluent and chlorinated secondary effluent within the plant, and from downstream receiving waters. Stream flow contribution and plant effluent contribution required the calculation of dilution factors to determine expected numbers of the various microflora found downstream assuming no residual chlorine effect. Total chlorine concentration was measured by amperometric titration at the …

An Immunochemical Study Of The Combining Sites Of The Second Lectin Isolated From Bandeiraea Simplicifolia (Bs Ii), Charles Wood, E. A. Kabat, S. Ebisu, Irwin J. Goldstein

Nebraska Center for Virology: Faculty Publications

Two lectins with different binding specificities have been isolated from extracts of seeds of Bandeiraea simplicifolia. The first, Bandeiraea lectin I [11] was specific for terminal non-reducing αDGalactosyl residues. It reacted with B substances from human ovarian cysts and with several galactomannans to form precipitin lines in agar gels. Polysaccharides with terminal αDGalactosyl residues, such as larch galactan, did not react. The lectin agglutinated B erythrocytes strongly but also reacted to a lower titre with A₁ and very weakly with A₂ erythrocytes [15, 28] indicating that terminal non-reducing αDGalNAc [24] can …

Presence Of An Unusual Eosinophilic Staining Substance In The Blood Of Snakes, James J. Daly Sr., Charles H. Calhoun

Journal of the Arkansas Academy of Science

No abstract provided.

An Electron Microscopic Investigation Of Bovine Abortion, Hans A. Draayer

Electronic Theses and Dissertations

Bovine abortion continues to cause economic loss to farmers and ranchers. In South Dakota, monetary loss from bovine abortion has been estimated at 4 to 4.8 million dollars annually. (31) Brucellosis was once considered the major cause of bovine abortion. Since the prevalence of brucellosis has been reduced to less than 1% of the total United States cattle population, there has been an increase in demand for laboratory assistance in the diagnosis of the causes of bovine abortion. With the present day technology in microbiology, histology, and serology, a confirmed identification of the causative agent of bovine abortion can be …

Bacterial Response To Crude Oil Spillage In A Salt Marsh, Russell Paul Herwig

Dissertations, Theses, and Masters Projects

No abstract provided.

Rescue Of Infectious Bk Virus From A T-Antigen Negative Bkv Transformed Bhk-21 Cell Line, David Michael Olive

Dissertations

No abstract provided.

Interactions In Lambdoid Bacteriophage Populations, Bruce Edward Mitchell

Masters Theses

The lambdoid bacteriophages are ideal subjects for the study of populational interactions at the molecular level because they do not mutually exclude one another in mixed infection of the same host cell. Previous work done in this laboratory using the lambdoid phage pairs λ-434 hy mi and λ-∅80 have demonstrated a density-dependent reduction in progeny yield per infected cell (burst size) as a function of increasing multiplicity of infection (m.o.i.). The λ cI gene which codes for the repress or molecule has been demonstrated to be the cause of the interaction in the λ-434 hy mi phage pair. …

Effect Of Radiation And Corynebacterium Parvum On The In Vivo Elimination Of Friend Virus-Induced Leukemia In C57bl/6 Mice, Betty A. Wu

Loma Linda University Electronic Theses, Dissertations & Projects

Administration of Corynebacterium parvum in combination with localized irradiation has been shown to be more efficient than radiation treatment alone in prolonging the survival time of methylethrane induced tumor in C3H mice. Fractionated low dose total body irradiation has been demonstrated to be a promising therapy for patients with chronic lymphocytic leukemia. The purpose of this study was to determine the effect of the combined treatment of total body irradiation and C. parvum on Friend virus induced leukemia in C57BL/6 mice. ^₁₂₅I-labeled FBL-3 cells were injected intraperitoneally into mice. Intraperitoneal injection of C. parvum and and single dose total …

Buoyancy Changes As Related To Respiratory Behavior In An Amphibious Snail, Pomacea Urceus (Müller), From Venezuela, Kathleen A. Burky, Albert J. Burky

Biology Faculty Publications

The ampullariid Pomacea urceus uses its ctenidium and lung in respiration. The snails reach the surface with their siphon and ventilate their lung by withdrawing their head-foot in a pumping action. The lung gas also serves to change the buoyancy of the snails. After a ventilation a snail does not necessarily return to the same buoyancy level. They may achieve overall specific gravities of <1 to > 1 after a ventilation. They may remain submerged (s.g. > 1) or float (s.g.

The Biosynthesis Of Mannitol And Mannitol 1-Phosphate In Staphylococci, Kenneth Edwards

Master's Theses

No abstract provided.

Absence Of Spermine In Filamentous Fungi, Kenneth W. Nickerson, Larry D. Dunkle, James L. Van Etten

Papers in Microbiology

Polyamines were examined in several yeasts and filamentous fungi. Whereas putrescine, spermidine, and spennine were present in the yeasts, spernine was not detected in any of the filamentous fungi.