Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 7 of 7

Full-Text Articles in Life Sciences

Sympatric Soil Microbe Interactions Between Streptomyces And Fusarium Isolates, Lehren A. Olk-Szost Jul 2023

Sympatric Soil Microbe Interactions Between Streptomyces And Fusarium Isolates, Lehren A. Olk-Szost

All NMU Master's Theses

Interkingdom interactions between soil bacteria and fungi may play a critical role in occurrence of disease suppressive soils, yet our understanding of these interactions remains limited. Streptomyces are well-known producers of antimicrobial compounds important to medicine and agriculture. Production of these secondary metabolites is often mediated by quorum sensing. Most Streptomyces research occurs in single species experiments, yet new metabolites have been discovered in interspecies co-culture experiments. Interspecies, intergenic, and interkingdom co-culture research will likely reveal many valuable compounds, and strengthen our understanding of complex ecological interactions in soil microbiomes. Interactions between sympatric Streptomyces and Fusarium isolates from disease suppressive …


Simple Organic Fertilizer Amendments For Farming In Degraded Soils: Effects On Plant-Microbe Interactions, Andrew Adamski Nov 2022

Simple Organic Fertilizer Amendments For Farming In Degraded Soils: Effects On Plant-Microbe Interactions, Andrew Adamski

All NMU Master's Theses

The rapid loss of topsoil, biodiversity, and water quality across agricultural land in the United States and the industrialized world poses some of the most important risks to the future of global and domestic food security. Not only is arable land being lost at an unsustainable pace, but the average age of farmers has also steadily been rising due to a myriad of barriers young, BIPOC (Black, Indigenous, and People of Color), and beginning farmers face. In an attempt to address these issues, worm castings, bokashi tea, fish hydrolysate, and biochar were applied alone and in combination to an extremely …


The Gut Microbiota Of A Wild American Black Bear (Ursus Americanus) Population, Sierra Gillman Mar 2020

The Gut Microbiota Of A Wild American Black Bear (Ursus Americanus) Population, Sierra Gillman

All NMU Master's Theses

The gut microbiome (GMB), the mutualistic microbial communities located in the gastrointestinal tract (GIT), has co-evolved in vertebrates to perform micro-ecosystem services to facilitate physiological functions. Despite the key role of the GMB in host health, wildlife managers have been slow to consider the ramifications of anthropogenic pressures to wildlife-GMB diversity. For example, although diet is one of the most influential determinants of a host’s GMB, many wildlife agencies allow baiting with human-provisioned foods to facilitate the harvest of wildlife such as American black bear (Ursus americanus). Additionally, much of our knowledge of wildlife-GMB relationships is based on …


Isolating And Identifying Fungal Endophytes From Roots Of Rare Orchid Cypripedium Arietinum In Grand Sable Dunes, Michigan, Kari Farkas-Lasich Jun 2018

Isolating And Identifying Fungal Endophytes From Roots Of Rare Orchid Cypripedium Arietinum In Grand Sable Dunes, Michigan, Kari Farkas-Lasich

All NMU Master's Theses

Recently the population of Cypripedium arietinum in Grand Sable Dunes, Michigan was estimated at more than 3.5 million plants, greater than 90% of the world’s known population. There is little research on this species’ root associated fungal endophytes. This new population estimate allows the ethical, destructive sampling required to shed light on root associated symbionts and further successful preservation and restoration of the species. Samples were collected of current year’s roots from 75 C. arietinum orchids at 75 locations within 4 jack pine forests in the Grand Sable Dunes, Pictured Rocks National Lakeshore, Michigan. Fungal endophyte cultures were obtained from …


Molecular Subtyping Of Staphylococcus Aureus Isolates From The U.P. Community For The Presence Of Toxin-Encoding Genes, Carol I. Kessel May 2017

Molecular Subtyping Of Staphylococcus Aureus Isolates From The U.P. Community For The Presence Of Toxin-Encoding Genes, Carol I. Kessel

All NMU Master's Theses

Staphylococcus aureus is the most common cause of human bacterial infections; however, humans can also be asymptomatically colonized with S. aureus. Asymptomatic carriers can potentially spread S. aureus infection to others. These infections can range from mild to severe. The pathology of a S. aureus infection is often dependent on which toxins are expressed and the virulence factors with which they are associated. One goal of this study was to isolate S. aureus from healthy, consenting adult volunteers who submitted nasal swabs for culture and qRT-PCR analysis to determine which strains are present in the community. This knowledge could …


A Novel And Rapid Staphylococcus Aureus Bacterial Identification Method Utilizing Immunomagnetic Beads And Single Cell Laser-Light Scattering, Kaylagh Hollen May 2016

A Novel And Rapid Staphylococcus Aureus Bacterial Identification Method Utilizing Immunomagnetic Beads And Single Cell Laser-Light Scattering, Kaylagh Hollen

All NMU Master's Theses

Staphylococcus aureus is the most commonly isolated human associated bacterial pathogen. It plays an important role in skin and soft-tissue infections, pneumonia, endocarditis, osteomyelitis, foreign-body infections, and sepsis. S. aureus diagnosis and treatment requires a minimum of 24-48. With this in mind, previous studies suggest that faster pathogen identification has been linked to improved patient outcomes. Improved patient outcomes including a reduction in hospitalization time, decreased risk of nosocomial infections, and decreased in medical costs. The impact of faster identification on patient outcome has led us to develop an alternative method of S. aureus identification via ImmunoMagnetic Separation (IMS) and …


Gene Regulation By A Novel Two-Component System Conserved Among Gammaproteobacteria, Kristin M. Jacob Aug 2015

Gene Regulation By A Novel Two-Component System Conserved Among Gammaproteobacteria, Kristin M. Jacob

All NMU Master's Theses

Two-component systems are common gene regulatory pathways seen in bacteria. Gene expression is controlled by these systems through a series of phosphorylation events between two proteins, a membrane bound sensor kinase and a cytoplasmic response regulator. Activation of a two-component system can be caused by biological or environmental stimuli, resulting in altered gene expression. A conserved two-component system was recently discovered in entomopathogenic bacteria, including our model organisms Pseudomonas entomophila and Pseudomonas aeruginosa, and is homologous to the recently identified two-component system, CrbRS, in Vibrio cholerae. In V. cholerae CrbRS regulates acetate metabolism and controls virulence. The aim of this …