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Articles 1 - 30 of 55
Full-Text Articles in Life Sciences
A Review Of Ministerial Policy Guidelines For Rock Lobster Processing In Western Australia From The Working Group Appointed By The Minister For Fisheries And Chaired By Peter Rich, Peter Rich
Fisheries management papers
The rock lobster processing industry in Western Australia has a long history of management. Whilst management of the industry through the Fisheries Department has obviously been successful in helping to build the rock lobster industry into one of the State's major export industries, this approach is not without problems. With the introduction of the Fisheries Management Act 1994, the Minister for Fisheries deemed it appropriate to appoint an expert group to review the existing regulation of the rock lobster processing sector, draft the necessary guidelines to assist the Executive Director of the Fisheries Department make decisions on rock lobster processing …
Connexin32 Is A Myelin-Related Protein In The Pns And Cns, Steven S. Scherer, Suzanne M. Deschênes, Yi-Tian Xu, Judith B. Grinspan, Kenneth H. Fischbeck, David L. Paul
Connexin32 Is A Myelin-Related Protein In The Pns And Cns, Steven S. Scherer, Suzanne M. Deschênes, Yi-Tian Xu, Judith B. Grinspan, Kenneth H. Fischbeck, David L. Paul
Biology Faculty Publications
We have examined the expression of a gap junction protein, connexin32 (Cx32), in Schwann cells and oligodendrocytes. In peripheral nerve, Cx32 is found in the paranodal myelin loops and Schmidt-Lanterman incisures of myelinating Schwann cells, and the levels of Cx32 protein and mRNA change in parallel with those of other myelin-related genes during development, Wallerian degeneration, and axonal regeneration. In the central nervous system, Cx32 is found in oligodendrocytes and their processes, but not in compact myelin, and the levels of Cx32 protein and mRNA increase during development in parallel with those of the other myelin genes. Thus, Cx32 is …
Active Inhibition Of T Cell Proliferation By Monocytes, Dean Anthony Lee
Active Inhibition Of T Cell Proliferation By Monocytes, Dean Anthony Lee
Loma Linda University Electronic Theses, Dissertations & Projects
Monoclonal antibodies (mAb) to MHC class II molecules (anti-class II mAb) have been well described in their ability to inhibit T cell activation. The most current model is that anti-class II mAb interfere directly with the T cell receptor-major histocompatibility complex crosslinking which is necessary for T cell activation. The anti-CD3 mAb, OKT3, is an MHC class II molecule-independent mitogen which causes T cell activation by direct ligation of the TCR-CD3 complex. OKT3 and a panel of anti-class II mAb were used to dissect the individual roles played by these two receptors in activation and inhibition of peripheral blood mononuclear …
Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter
Involvement Of P59FynT In Interleukin-5 Receptor Signaling, Mark W. Appleby, James D. Kerner, Sylvia Chien, Charles R. Maliszewski, Subbarao Bondada, Roger M. Perlmutter
Microbiology, Immunology, and Molecular Genetics Faculty Publications
Previous studies implicate the nonreceptor protein tyrosine kinase (PTK) p59fyn in the propagation of signals from the B cell antigen receptor. To elucidate the functions of this kinase, we examined B cell responsiveness in mice engineered to lack the hematopoietic isoform of p59fyn. Remarkably, antigen receptor signaling was only modestly defective in fynTnull B cells. In contrast, signaling from the interleukin (IL)-5 receptor which ordinarily provides a comitogenic stimulus with antiimmunoglobulin, was completely blocked. Our results document the importance of p59fynT in IL-5 responses in B cells, and they support a general model …
The Impact Of The New Management Package On Smaller Operators In The Western Rock Lobster Fishery, Ross Gould
The Impact Of The New Management Package On Smaller Operators In The Western Rock Lobster Fishery, Ross Gould
Fisheries management papers
For the 1993/94 western rock lobster fishing season several new management strategies were introduced. The objective of the changes to the management package was to protect the rock lobster fishery. It was also to provide sufficient breeding stock to ensure viability of the industry. The most significant new measure was a temporary (two seasons) 18 percent pot reduction. Other changes were also made. There were considered sound reasons for reviewing and changing the previous management package. Despite this, some fishermen were critical of the new management package, and claimed that it unfairly disadvantaged smaller operators. Against this background, the Department …
Recombinant Soluble Epoxide Hydrolase, Bruce D. Hammock, David F. Grant, Jeffrey K. Beetham
Recombinant Soluble Epoxide Hydrolase, Bruce D. Hammock, David F. Grant, Jeffrey K. Beetham
Jeffrey K. Beetham
The present invention relates to nucleic acid sequences and methods useful for producing recombinant human soluble epoxide hydrolase (sEH).
West Coast Rock Lobster Fishery Management Plan 1995 - Draft For Public Comment, M. Moran
West Coast Rock Lobster Fishery Management Plan 1995 - Draft For Public Comment, M. Moran
Fisheries management papers
The purpose of this management plan is to regulate fishing for rock lobsters (Family Palimuridae) in waters off the west coast of Western Australia such that the breeding stock of rock lobsters is maintained at a level known to have generated adequate recruitment of young lobsters in the past. This management plan does not constitute a change in the management arrangements for the rock lobster fishery. It essentially replaces the West Coast Rock Lobster Limited Entry Fishery Notice 1993 made under the Fisheries Act 1905 and makes the required changes to wording and structure necessitated by the proclamation of the …
Bag And Size Limit Fishing Regulations From Around Australia - Current Information As At 1 July, 1995 - Third Australasian Fisheries Managers Conference, Rottnest Island, Wa, F. B. Prokop
Fisheries management papers
This paper was originally prepared for the first National Fisheries Managers Conference in 1993. It is now updated and includes the current bag and size limits from around Australia as at 1 July, 1995. It is fairly obvious from this paper that there is a need for more cooperation between the states, particularly for species which have wide distributions. However, regional solutions can be a good approach for specific management needs, while general rules can be made more consistent in a number of cases.
Developmental Regulation Of The Murine Cd4 Gene, Rhonda Kay Hansen
Developmental Regulation Of The Murine Cd4 Gene, Rhonda Kay Hansen
Loma Linda University Electronic Theses, Dissertations & Projects
CD4 is a membrane bound glycoprotein that plays an important role in T cell development and activation. An extensive analysis of the CD4 locus identified potential transcriptional control elements located 5' of the promoter and within the first intron. Since the molecular mechanism for the control of CD4 expression during T cell development had not been determined, it is the focus of this dissertation. A series of recombinant DNA constructs were made to determine the contribution of putative transcriptional control elements in regard to CD4 expression, and to identify elements being used to control CD4 expression during T cell development. …
The Heterochronic Gene Lin-29 Encodes A Zinc Finger Protein That Controls A Terminal Differentiation Event In Caenorhabditis Elegans, Ann Rougvie, Victor Ambros
The Heterochronic Gene Lin-29 Encodes A Zinc Finger Protein That Controls A Terminal Differentiation Event In Caenorhabditis Elegans, Ann Rougvie, Victor Ambros
Victor R. Ambros
A hierarchy of heterochronic genes, lin-4, lin-14, lin-28 and lin-29, temporally restricts terminal differentiation of Caenorhabditis elegans hypodermal seam cells to the final molt. This terminal differentiation event involves cell cycle exit, cell fusion and the differential regulation of genes expressed in the larval versus adult hypodermis. lin-29 is the most downstream gene in the developmental timing pathway and thus it is the most direct known regulator of these diverse processes. We show that lin-29 encodes a protein with five zinc fingers of the (Cys)2-(His)2 class and thus likely controls these processes by regulating transcription in a stage-specific manner. Consistent …
The Caenorhabditis Elegans Heterochronic Gene Pathway Controls Stage-Specific Transcription Of Collagen Genes, Zhongchi Liu, Susan Kirch, Victor Ambros
The Caenorhabditis Elegans Heterochronic Gene Pathway Controls Stage-Specific Transcription Of Collagen Genes, Zhongchi Liu, Susan Kirch, Victor Ambros
Victor R. Ambros
In Caenorhabditis elegans, the terminal differentiation of the hypodermal cells occurs at the larval-to-adult molt, and is characterized in part by the formation of a morphologically distinct adult cuticle. The timing of this event is controlled by a pathway of heterochronic genes that includes the relatively direct regulatory gene, lin-29, and upstream genes lin-4, lin-14 and lin-28. Using northern analysis to detect endogenous collagen mRNA levels and collagen/lacZ reporter constructs to monitor collagen transcriptional activity, we show that the stage-specific switch from larval cuticle to adult cuticle correlates with the transcriptional activation of adult-specific collagen genes and repression of larval-specific …
Translocation Issues In Western Australia: Proceedings Of A Seminar And Workshop Held On 26th And 27th September 1994, F. B. Prokop
Translocation Issues In Western Australia: Proceedings Of A Seminar And Workshop Held On 26th And 27th September 1994, F. B. Prokop
Fisheries management papers
The Recreational Fishing Advisory Committee (RFAC) approved holding this translocation seminar to air the translocation of fish for recreational enhancement in a public forum. This forum will identify issues including establishing common ground between the variety of interest groups which are represented. Contentious areas which require further investigation and consultation should become apparent, and assist the formulation of appropriate policies on translocation for Western Australia.
Identification And Characterization Of Mitochondrial Dna Variants In Alzheimer's Disease, Natasha Singh Hamblet
Identification And Characterization Of Mitochondrial Dna Variants In Alzheimer's Disease, Natasha Singh Hamblet
Theses and Dissertations in Biomedical Sciences
Alzheimer's Disease (AD) is a complex neurodegenerative disorder that affects a significant portion of the human population regardless of ethnicity or gender. A mitochondrial hypothesis of AD has been proposed based on a number of studies which establish altered oxidative phosphorylation (OXPHOS) and ATP synthesis in AD tissue. ATP demand is most prevalent in the brain; damage to OXPHOS could severely impair brain metabolism, thereby leading to a decline in cognitive function. Four out of five complexes in the OXPHOS pathway are partly encoded by mitochondrial DNA (mtDNA); thus, this may be a crucial site of lesions that alter brain …
Investigation Of The Substrate Recognition Characteristics And Kinetics Of Mammalian Mitochondrial Dna Topoisomerase I, Zeki Topcu
Theses and Dissertations in Biomedical Sciences
Topoisomerases are DNA-modifying enzymes found in prokaryotes, eukaryotes, viruses and organelles such as chloroplast and mitochondria. Information about these enzymes in eukaryotic systems is mostly limited to nuclear enzymes, although our laboratory has been characterizing the biochemical and biophysical properties of the mammalian mitochondrial topoisomerases. We have determined the polarity of the attachment of mitochondrial topoisomerase I to its substrate DNA. To study the substrate preference and kinetic parameters of mitochondrial topoisomerase I, selected regions of mammalian mitochondrial DNA (mtDNA) were inserted into pGEM plasmid vectors following a series of modification and optimization experiments of currently available methods for PCR-cloning. …
Management Of The Northern Demersal Scalefish Fishery, Jane Fowler
Management Of The Northern Demersal Scalefish Fishery, Jane Fowler
Fisheries management papers
In mid 1994, the Fisheries Department of Western Australia announced that it would prepare a discussion paper setting out options for future management of the demersal scalefish fishery off the Kimberley coast. This paper, which was to be the start of a wider consultative process, was also to include options for possible further development of the trap and line fisheries. This paper is only the first step in a process for developing formal long term management arrangements under the new fisheries legislation, the Fish Resources Management Act 1994. Its aim is to provide some background and to present some management …
Report On Future Management Options For The South West Trawl Limited Entry Fishery, South West Trawl Limited Entry Fishery Working Group
Report On Future Management Options For The South West Trawl Limited Entry Fishery, South West Trawl Limited Entry Fishery Working Group
Fisheries management papers
As a result of community concern regarding trawling, the current management rules for the South West Trawl Limited Entry Fishery were finalised in February 1989 and the final plan was gazetted in October of that year. However, there was public disquiet about this trawling, and as a result of this disquiet, a research program was initiated by the Fisheries Department with funding received from both the Commonwealth and State Governments. The scientific report, "The impact of trawling for saucer scallops and western king prawns on the benthic communities in coastal waters off south-western Australia" by L J B Laurenson, P …
Management Options (Discussion Paper) For The Shark Bay Snapper Limited Entry Fishery, Shark Bay Snapper Limited Entry Fishery Working Group, Doug Bathgate
Management Options (Discussion Paper) For The Shark Bay Snapper Limited Entry Fishery, Shark Bay Snapper Limited Entry Fishery Working Group, Doug Bathgate
Fisheries management papers
At the Annual Management Meeting of the Shark Bay Snapper Limited Entry Fishery held on 22 July 1994 industry put forward a proposal that supplementary and non-transferable quota that was "lost" to the fishery when a licence holding non-transferable quota was transferred should be retained by the Fisheries Department and leased back to licence holders on an annual basis. As a result of this proposal and the fact that the management arrangements for the fishery are extremely complex and in need of simplification, the Minster for Fisheries approved the establishment of a Working Group to examine the lease-back proposal and …
Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou
Regulation Of The Insulin-Like Growth Factor System By Retinoic Acid In Human Osteoblast Cells, Yuehua Zhou
Loma Linda University Electronic Theses, Dissertations & Projects
Retinoic acid (RA) is an important regulator of growth and differentiation in many cell types, including bone. However, its effects on human osteoblast cell growth and differentiation have not been well studied. Therefore, I investigated the effect of RA on proliferation and differentiation of normal human bone cells (HBCs) and human osteosarcoma, SaOS-2 cells. RA decreased baseline as well as serum-stimulated proliferation in normal HBCs. To determine the effect of RA on differentiation, expression of several osteoblastic differentiation markers were studied. RA decreased type I procollagen mRNA levels and l,25(OH)2D3-stimulated osteocalcin and alkaline phosphatase (ALP) mRNA …
A Model Of Cytomegalovirus Association With The Development Of Heart Graft Atherosclerosis, Evan Skowronski
A Model Of Cytomegalovirus Association With The Development Of Heart Graft Atherosclerosis, Evan Skowronski
Loma Linda University Electronic Theses, Dissertations & Projects
The development of graft atherosclerosis in heart transplant recipients has been associated with the development of post-transplant cytomegalovirus infection. The mechanism of this involvement, however, remains unclear. I undertook a study to determine the role of cytomegalovirus in the development of graft atherosclerosis. First, a rapid method of detecting cytomegalovirus based on capillary polymerase chain reaction and gel electrophoresis was employed to determine the presence of cytomegalovirus in a wide variety of clinical samples. Detection of cytomegalovirus is a lengthy procedure under normal conditions, and timely detection of cytomegalovirus in transplant recipients may allow intervention early enough to block the …
3 Genes Of The Map Kinase Cascade, Mek-2, Mpk-1/Sur-1 And Let-60 Ras, Are Required For Meiotic Cell-Cycle Progression In Caenorhabditis-Elegans, Diane L. Church, Kun Liang Guan, Eric J. Lambie
3 Genes Of The Map Kinase Cascade, Mek-2, Mpk-1/Sur-1 And Let-60 Ras, Are Required For Meiotic Cell-Cycle Progression In Caenorhabditis-Elegans, Diane L. Church, Kun Liang Guan, Eric J. Lambie
Dartmouth Scholarship
In the germline of Caenorhabditis elegans hermaphrodites, meiotic cell cycle progression occurs in spatially restricted regions. Immediately after leaving the distal mitotic region, germ cells enter meiosis and thereafter remain in the pachytene stage of first meiotic prophase for an extended period. At the dorsoventral gonadal flexure, germ cells exit pachytene and subsequently become arrested in diakinesis. We have found that exit from pachytene is dependent on the function of three members of the MAP kinase signaling cascade. One of these genes, mek-2, is a newly identified C. elegans MEK (MAP kinase kinase). The other two genes, mpk-1/sur-1 (MAP kinase) …
The Bag And Size Limit Review: New Regulations And Summary Of Submissions, F. B. Prokop, J M. Collins
The Bag And Size Limit Review: New Regulations And Summary Of Submissions, F. B. Prokop, J M. Collins
Fisheries management papers
Since the new bag and size limits were introduced in December 1991, there have been many suggestions for refinement and improvement of these regulations. This paper includes the summary of submissions to Fisheries Management Paper No 52 - Review of Bag and Size Limit Proposals for Western Australian Recreational Fishers and details new regulations which will come into force on 1 July 1995.
The Best Available Information - Its Implications For Recreational Fisheries Management, F. B. Prokop
The Best Available Information - Its Implications For Recreational Fisheries Management, F. B. Prokop
Fisheries management papers
Workshop at Second National Fisheries Managers Conference, Bribie Island, Queensland, October 18, 1994. Recreational fisheries management is one of the growth areas in Australian fisheries. The Australian Society for Fish Biology Workshop topic for its 1994 Conference was Recreational Fishing - What's the Catch. It was acknowledged at the ASFB conference that there were generally poor levels of information and for the lower value commercial and recreational fisheries there may be no way to gather definitive research data for the foreseeable future. However, in even the high profile fisheries, public perceptions, attitudes, and political will do not allow for the …
Plant Community Changes Over 54 Years Within The Great Basin Experimental Range, Manti-La Sal National Forest, Scott C. Walker, David K. Mann, E. Durant Mcarthur
Plant Community Changes Over 54 Years Within The Great Basin Experimental Range, Manti-La Sal National Forest, Scott C. Walker, David K. Mann, E. Durant Mcarthur
Aspen Bibliography
Plant community changes and natural succession over time impact forage values, watershed quality, wildlife habitat, and ecosystem dynamics. Comparisons were made between a vegetation map of community types completed in 1937 by the U.S. Forest Service, and vegetation maps compiled in 1990 of the same areas by satellite imagery, and through 1991 areal photo interpretation combined with ground truthing. The study area includes nearly all of the drainage in Ephraim Canyon located in central Utah which consists of 6,027 acres (2,439 ha). Elevation ranges from 6,600 to 10,400 feet (2,040 to 3,210 m). Vegetation types ranged from pinyon-juniper woodland through …
Identification And Characterization Of Genes Associated With V-Jun Induced Cell Transformation, Martin Toralballa Hadman
Identification And Characterization Of Genes Associated With V-Jun Induced Cell Transformation, Martin Toralballa Hadman
Biological Sciences Theses & Dissertations
The v-jun oncogene was initially identified as the causative agent for fibrosarcomas in chickens. Studies show that overexpression of v-Jun proteins transforms chicken embryo fibroblasts (CEF) in vitro, and forms tumors in chickens in vivo. The mechanisms for this are not clearly defined. Conceivably, overexpression of an unregulated transcription factor would cause cell transfonnation by illicit regulation of its target genes. In support of this, we show that in vivo v-Jun complexes exhibit differential binding to in vitro generated AP-1 and 'AP-1 like' target sequences, suggesting that the pattern of target gene expression is altered during cell transformation. …
Pathogenicity Of Murine Cytomegalovirus Mutants, Victoria Jean Cavanaugh
Pathogenicity Of Murine Cytomegalovirus Mutants, Victoria Jean Cavanaugh
Theses and Dissertations in Biomedical Sciences
The purpose of this study was to identified nonessential murine cytomegalovirus (MCMV) genes involved in pathogenesis in vivo. Our approach to identifyjng these genes consisted of constructing MCMV mutants, and then analyzing these mutants in vitro and in vivo. Recombinant viruses (RV) expressing the β-glucuronidase marker gene were constructed by site-directed insertion and deletion mutagenesis of the MCMV Hind III-J and -I regions of the viral genome. Mutations were targeted to this region of the MCMV genome because the corresponding region of the human CMV genome is nonessential and is involved in down-regulating major histocompatibility complex (MHC) class I expression …
Characterization Of A Saccharomyces Cerevisiae Strain Deleted For The Rad27 Gene; A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Wolfram Siede, Errol C. Friedberg
Characterization Of A Saccharomyces Cerevisiae Strain Deleted For The Rad27 Gene; A Structural Homolog Of The Rad2 Nucleotide Excision Repair Gene, Michael S. Reagan, Wolfram Siede, Errol C. Friedberg
Biology Faculty Publications
No abstract provided.
Enhancer Trap Technique: A Novel Tool For Identification And Developmental Characterization Of Genes Of Drosophila, Amit Singh
Biology Faculty Publications
The classical technique of mutational screen for identification of genes controlling early development has now approached saturation. A new era in genetic identification and developmental characterization of genes in Drosophila has commenced with the advent of the enhancer trap technique. This technique involves mobilization of a P-lacZ vector to diverse chromosomal locations in the fruit fly genome to bring it under the regulation of developmentally expressed genes or their enhancer elements. The technique offers a strikingly elegant method of gaining entry into fruit fly genes.
Draft Report Of The South Coast Estuarine Fishery Working Group, South Coast Estuarine Fishery Workng Group
Draft Report Of The South Coast Estuarine Fishery Working Group, South Coast Estuarine Fishery Workng Group
Fisheries management papers
The South Coast Estuarine Working Group was established in 1991 at the request of commercial fishermen by the Minister of the day. This report outlines a draft management plan for the South Coast Estuarine fishery that was made available for public comment.
Analyzing Correlations Of Three Types In Selected Lines Of Drosophila Melanogaster That Have Evolved Stable Extreme Geotactic Performance, Scott F. Stoltenberg, Jerry Hirsch, Stewart H. Berlocher
Analyzing Correlations Of Three Types In Selected Lines Of Drosophila Melanogaster That Have Evolved Stable Extreme Geotactic Performance, Scott F. Stoltenberg, Jerry Hirsch, Stewart H. Berlocher
Department of Psychology: Faculty Publications
The behavior-genetic analysis of Drosophila melanogaster with geotactic performance as the phenotype is an ideal model system with which to investigate the complex relations between heredity and behavior. As part of a long-term, 38-year study, we report 4 experiments that identify and analyze trait correlations in the selected high- and low-geotaxis lines. We performed F2 correlational analyses and backcrosses to examine 3 types of correlations: (a) genotype-genotype (alcohol dehydrogenase [Adh]-amylase [Amy]), (b) genotype-phenotype (Adh and Amy-geotaxis), and (c) phenotype-phenotype (mate preference–geotaxis). Only the Adh-geotaxis correlation survived meiosis and reappeared in the F …
Percina Austroperca: A New Species Of Logperch (Percidae, Subgenus Percina) From The Choctawhatchee And Escambia Rivers In Alabama And Florida, Bruce A. Thompson
Percina Austroperca: A New Species Of Logperch (Percidae, Subgenus Percina) From The Choctawhatchee And Escambia Rivers In Alabama And Florida, Bruce A. Thompson
Occasional Papers of the Museum of Natural Science, Louisiana State University
No abstract provided.