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Environmental Microbiology and Microbial Ecology
Polymerase-Chain-Reaction; Clam Tapes-Decussatus; Ribosomal-Rna Locus; Crassostrea-Virginica; Quantitative PCR
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Full-Text Articles in Life Sciences
Real-Time Pcr For Detection And Quantification Of The Protistan Parasite Perkinsus Marinus In Environmental Waters, Corinne Audemard, Kimberly S. Reece, Em Burreson
Real-Time Pcr For Detection And Quantification Of The Protistan Parasite Perkinsus Marinus In Environmental Waters, Corinne Audemard, Kimberly S. Reece, Em Burreson
VIMS Articles
The protistan parasite Perkinsus marinus is a severe pathogen of the oyster Crassostrea virginica along the east coast of the United States. Very few data have been collected, however, on the abundance of the parasite in environmental waters, limiting our understanding of P. marinus transmission dynamics. Real-time PCR assays with SybrGreen I as a label for detection were developed in this study for quantification of P. marinus in environmental waters with P. marinus species-specific primers and of Perkinsus spp. with Perkinsus genus-specific primers. Detection of DNA concentrations as low as the equivalent of 3.3 x 10(-2) cell per 10-mul reaction …