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Articles 1 - 30 of 74
Full-Text Articles in Life Sciences
Grnsight: A Web Application And Service For Visualizing Models Of Small- To Medium-Scale Gene Regulatory Networks, Kam D. Dahlquist, John David N. Dionisio, Ben G. Fitzpatrick, Nicole A. Anguiano, Anindita Varshneya, Britain J. Southwick, Mihir Samdarshi
Grnsight: A Web Application And Service For Visualizing Models Of Small- To Medium-Scale Gene Regulatory Networks, Kam D. Dahlquist, John David N. Dionisio, Ben G. Fitzpatrick, Nicole A. Anguiano, Anindita Varshneya, Britain J. Southwick, Mihir Samdarshi
John David N. Dionisio
GRNsight is a web application and service for visualizing models of gene regulatory networks (GRNs). A gene regulatory network (GRN) consists of genes, transcription factors, and the regulatory connections between them which govern the level of expression of mRNA and protein from genes. The original motivation came from our efforts to perform parameter estimation and forward simulation of the dynamics of a differential equations model of a small GRN with 21 nodes and 31 edges. We wanted a quick and easy way to visualize the weight parameters from the model which represent the direction and magnitude of the influence of …
Preliminary Investigation Of Walking Motion Using A Combination Of Image And Signal Processing, Bradley Schneider, Tanvi Banerjee
Preliminary Investigation Of Walking Motion Using A Combination Of Image And Signal Processing, Bradley Schneider, Tanvi Banerjee
Kno.e.sis Publications
We present the results of analyzing gait motion in first-person video taken from a commercially available wearable camera embedded in a pair of glasses. The video is analyzed with three different computer vision methods to extract motion vectors from different gait sequences from four individuals for comparison against a manually annotated ground truth dataset. Using a combination of signal processing and computer vision techniques, gait features are extracted to identify the walking pace of the individual wearing the camera as well as validated using the ground truth dataset. Our preliminary results indicate that the extraction of activity from the video …
A Framework For The Statistical Analysis Of Mass Spectrometry Imaging Experiments, Kyle Bemis
A Framework For The Statistical Analysis Of Mass Spectrometry Imaging Experiments, Kyle Bemis
Open Access Dissertations
Mass spectrometry (MS) imaging is a powerful investigation technique for a wide range of biological applications such as molecular histology of tissue, whole body sections, and bacterial films , and biomedical applications such as cancer diagnosis. MS imaging visualizes the spatial distribution of molecular ions in a sample by repeatedly collecting mass spectra across its surface, resulting in complex, high-dimensional imaging datasets. Two of the primary goals of statistical analysis of MS imaging experiments are classification (for supervised experiments), i.e. assigning pixels to pre-defined classes based on their spectral profiles, and segmentation (for unsupervised experiments), i.e. assigning pixels to newly …
Investigating High Speed Localization Microscopy Through Experimental Methods, Data Processing Methods, And Applications Of Localization Microscopy To Biological Questions, Andrew J. Nelson
Electronic Theses and Dissertations
Fluorescence Photoactivation Localization Microscopy(FPALM) and other super resolution localization microscopy techniques can resolve structures with nanoscale resolution. Unlike techniques of electron microscopy, they are also compatible with live cell and live animal studies, making FPALM and related techniques ideal for answering questions about the dynamic nature of molecular biology in living systems. Many processes in biology occur on rapid sub second time scales requiring the imaging technique to be capable of resolving these processes not just with a high enough spatial resolution, but with an appropriate temporal resolution. To that end, this Dissertation in part investigates high speed FPALM as …
Stage-Specific Predictive Models For Cancer Survivability, Elham Sagheb Hossein Pour
Stage-Specific Predictive Models For Cancer Survivability, Elham Sagheb Hossein Pour
Theses and Dissertations
Survivability of cancer strongly depends on the stage of cancer. In most previous works, machine learning survivability prediction models for a particular cancer, were trained and evaluated together on all stages of the cancer. In this work, we trained and evaluated survivability prediction models for five major cancers, together on all stages and separately for every stage. We named these models joint and stage-specific models respectively. The obtained results for the cancers which we investigated reveal that, the best model to predict the survivability of the cancer for one specific stage is the model which is specifically built for that …
Heat Map Analysis Of Rna-Seq Data Using Rstudio, Ray A. Enke, Ashton Holub
Heat Map Analysis Of Rna-Seq Data Using Rstudio, Ray A. Enke, Ashton Holub
Ray Enke Ph.D.
Intro To Rstudio, Ray A. Enke, Ashton Holub
Intro To Rstudio, Ray A. Enke, Ashton Holub
Ray Enke Ph.D.
Network Inference Driven Drug Discovery, Gergely Zahoránszky-Kőhalmi, Tudor I. Oprea Md, Phd, Cristian G. Bologa Phd, Subramani Mani Md, Phd, Oleg Ursu Phd
Network Inference Driven Drug Discovery, Gergely Zahoránszky-Kőhalmi, Tudor I. Oprea Md, Phd, Cristian G. Bologa Phd, Subramani Mani Md, Phd, Oleg Ursu Phd
Biomedical Sciences ETDs
The application of rational drug design principles in the era of network-pharmacology requires the investigation of drug-target and target-target interactions in order to design new drugs. The presented research was aimed at developing novel computational methods that enable the efficient analysis of complex biomedical data and to promote the hypothesis generation in the context of translational research. The three chapters of the Dissertation relate to various segments of drug discovery and development process.
The first chapter introduces the integrated predictive drug discovery platform „SmartGraph”. The novel collaborative-filtering based algorithm „Target Based Recommender (TBR)” was developed in the framework of this …
Towards Deeper Understanding In Neuroimaging, Rex Devon Hjelm
Towards Deeper Understanding In Neuroimaging, Rex Devon Hjelm
Computer Science ETDs
Neuroimaging is a growing domain of research, with advances in machine learning having tremendous potential to expand understanding in neuroscience and improve public health. Deep neural networks have recently and rapidly achieved historic success in numerous domains, and as a consequence have completely redefined the landscape of automated learners, giving promise of significant advances in numerous domains of research. Despite recent advances and advantages over traditional machine learning methods, deep neural networks have yet to have permeated significantly into neuroscience studies, particularly as a tool for discovery. This dissertation presents well-established and novel tools for unsupervised learning which aid in …
Fractal Analysis Of Dna Sequences, Christian G. Arias, Pedro Antonio Moreno Phd, Carlos Tellez
Fractal Analysis Of Dna Sequences, Christian G. Arias, Pedro Antonio Moreno Phd, Carlos Tellez
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Teaching Systems Biology Of The Circadian Clock With Journal Articles And Matlab, Stephanie R. Taylor
Teaching Systems Biology Of The Circadian Clock With Journal Articles And Matlab, Stephanie R. Taylor
Annual Symposium on Biomathematics and Ecology Education and Research
No abstract provided.
Fedrr: Fast, Exhaustive Detection Of Redundant Hierarchical Relations For Quality Improvement Of Large Biomedical Ontologies, Guangming Xing, Guo-Qiang Zhang, Licong Cui
Fedrr: Fast, Exhaustive Detection Of Redundant Hierarchical Relations For Quality Improvement Of Large Biomedical Ontologies, Guangming Xing, Guo-Qiang Zhang, Licong Cui
Institute for Biomedical Informatics Faculty Publications
Background: Redundant hierarchical relations refer to such patterns as two paths from one concept to another, one with length one (direct) and the other with length greater than one (indirect). Each redundant relation represents a possibly unintended defect that needs to be corrected in the ontology quality assurance process. Detecting and eliminating redundant relations would help improve the results of all methods relying on the relevant ontological systems as knowledge source, such as the computation of semantic distance between concepts and for ontology matching and alignment.
Results: This paper introduces a novel and scalable approach, called FEDRR – Fast, Exhaustive …
A Pilot Study Of Comparison Gesture Analysis In Motion Driven Video Games, Fabrizio Valerio Covone, Brian Vaughan, Charlie Cullen
A Pilot Study Of Comparison Gesture Analysis In Motion Driven Video Games, Fabrizio Valerio Covone, Brian Vaughan, Charlie Cullen
Conference Papers
This study investigates whether there are significant differences in the gestures made by gamers and non-gamers whilst playing commercial games that employ gesture inputs. Specifically, the study focuses on testing a prototype of multimodal capture tool that we used to obtain real-time audio, video and skeletal gesture data. Additionally, we developed an experimental design framework for the acquisition of spatio-temporal gesture data and analysed the vector magnitude of a gesture to compare the relative displacement of each participant whilst playing a game.
User-Centered Design Of Multi-Gene Sequencing Panel Reports For Clinicians., Elizabeth Cutting, Meghan Banchero, Amber L Beitelshees, James J Cimino, Guilherme Del Fiol, Ayse P Gurses, Mark A Hoffman, Linda Jo Bone Jeng, Kensaku Kawamoto, Mark Kelemen, Harold Alan Pincus, Alan R Shuldiner, Marc S Williams, Toni I Pollin, Casey Lynnette Overby
User-Centered Design Of Multi-Gene Sequencing Panel Reports For Clinicians., Elizabeth Cutting, Meghan Banchero, Amber L Beitelshees, James J Cimino, Guilherme Del Fiol, Ayse P Gurses, Mark A Hoffman, Linda Jo Bone Jeng, Kensaku Kawamoto, Mark Kelemen, Harold Alan Pincus, Alan R Shuldiner, Marc S Williams, Toni I Pollin, Casey Lynnette Overby
Manuscripts, Articles, Book Chapters and Other Papers
The objective of this study was to develop a high-fidelity prototype for delivering multi-gene sequencing panel (GS) reports to clinicians that simulates the user experience of a final application. The delivery and use of GS reports can occur within complex and high-paced healthcare environments. We employ a user-centered software design approach in a focus group setting in order to facilitate gathering rich contextual information from a diverse group of stakeholders potentially impacted by the delivery of GS reports relevant to two precision medicine programs at the University of Maryland Medical Center. Responses from focus group sessions were transcribed, coded and …
Computerized Classification Of Surface Spikes In Three-Dimensional Electron Microscopic Reconstructions Of Viruses, Younes Benkarroum
Computerized Classification Of Surface Spikes In Three-Dimensional Electron Microscopic Reconstructions Of Viruses, Younes Benkarroum
Dissertations, Theses, and Capstone Projects
The purpose of this research is to develop computer techniques for improved three-dimensional (3D) reconstruction of viruses from electron microscopic images of them and for the subsequent improved classification of the surface spikes in the resulting reconstruction. The broader impact of such work is the following.
Influenza is an infectious disease caused by rapidly-changing viruses that appear seasonally in the human population. New strains of influenza viruses appear every year, with the potential to cause a serious global pandemic. Two kinds of spikes – hemagglutinin (HA) and neuraminidase (NA) – decorate the surface of the virus particles and these proteins …
Algorithms For Glycan Structure Identification With Tandem Mass Spectrometry, Weiping Sun
Algorithms For Glycan Structure Identification With Tandem Mass Spectrometry, Weiping Sun
Electronic Thesis and Dissertation Repository
Glycosylation is a frequently observed post-translational modification (PTM) of proteins. It has been estimated over half of eukaryotic proteins in nature are glycoproteins. Glycoprotein analysis plays a vital role in drug preparation. Thus, characterization of glycans that are linked to proteins has become necessary in glycoproteomics. Mass spectrometry has become an effective analytical technique for glycoproteomics analysis because of its high throughput and sensitivity. The large amount of spectral data collected in a mass spectrometry experiment makes manual interpretation impossible and requires effective computational approaches for automated analysis. Different algorithmic solutions have been proposed to address the challenges in glycoproteomics …
Grnsight: A Web Application And Service For Visualizing Models Of Small- To Medium-Scale Gene Regulatory Networks, Kam D. Dahlquist, John David N. Dionisio, Ben G. Fitzpatrick, Nicole A. Anguiano, Anindita Varshneya, Britain J. Southwick, Mihir Samdarshi
Grnsight: A Web Application And Service For Visualizing Models Of Small- To Medium-Scale Gene Regulatory Networks, Kam D. Dahlquist, John David N. Dionisio, Ben G. Fitzpatrick, Nicole A. Anguiano, Anindita Varshneya, Britain J. Southwick, Mihir Samdarshi
Biology Faculty Works
GRNsight is a web application and service for visualizing models of gene regulatory networks (GRNs). A gene regulatory network (GRN) consists of genes, transcription factors, and the regulatory connections between them which govern the level of expression of mRNA and protein from genes. The original motivation came from our efforts to perform parameter estimation and forward simulation of the dynamics of a differential equations model of a small GRN with 21 nodes and 31 edges. We wanted a quick and easy way to visualize the weight parameters from the model which represent the direction and magnitude of the influence of …
Bayesian Networks To Assess The Newborn Stool Microbiome, William E. Bennett Jr.
Bayesian Networks To Assess The Newborn Stool Microbiome, William E. Bennett Jr.
McKelvey School of Engineering Theses & Dissertations
In human stool, a large population of bacterial genes and transcripts from hundreds of genera coexist with host genes and transcripts. Assessments of the metagenome and transcriptome are particularly challenging, since there is a great deal of sequence overlap among related species and related genes. We sequenced the total RNA content from stool samples in a neonate using previously-described methods. We then performed stepwise alignment of different populations of RNA sequence reads to different indices, including ribosomal databases, the human genome, and all sequenced bacterial genomes. Each pool of RNA at each alignment step was subjected to compression to assess …
Ifly: Code Development For An App To Support Automating Entomological Data Collection, Michael P. Cosentino, Trevor Stamper
Ifly: Code Development For An App To Support Automating Entomological Data Collection, Michael P. Cosentino, Trevor Stamper
The Summer Undergraduate Research Fellowship (SURF) Symposium
We are developing a prototype entomological data-collection application called "iFly," which runs on a field-capable iPad device. In this phase, we tackled refining screens and introducing a database manager to streamline operations as info is entered, stored, retrieved and delivered. We used SQLite3 database in Apple's Xcode Integrated Development Environment (IDE). Xcode gives mixed programming results. Apple's iOS environment ensures functional and fairly error-free apps can be built. But the sophisticated Xcode IDE requires specialist developers and valuable project time is spent as new programmers learn key techniques. The iFly prototype was advanced with improved database integration; however, more work …
A Gene-Based Association Method For Mapping Traits Using Reference Transcriptome Data, Eric R. Gamazon, Heather Wheeler, Kaanan P. Shah, Sahar V. Mozaffari, Keston Aquino-Michaels, Robert J. Carroll, Anne E. Eyler, Joshua C. Denny, Dan L. Nicolae, Nancy J. Cox, Hae Kyung Im
A Gene-Based Association Method For Mapping Traits Using Reference Transcriptome Data, Eric R. Gamazon, Heather Wheeler, Kaanan P. Shah, Sahar V. Mozaffari, Keston Aquino-Michaels, Robert J. Carroll, Anne E. Eyler, Joshua C. Denny, Dan L. Nicolae, Nancy J. Cox, Hae Kyung Im
Heather Wheeler
Genome-wide association studies (GWAS) have identified thousands of variants robustly associated with complex traits. However, the biological mechanisms underlying these associations are, in general, not well understood. We propose a gene-based association method called PrediXcan that directly tests the molecular mechanisms through which genetic variation affects phenotype. The approach estimates the component of gene expression determined by an individual’s genetic profile and correlates ‘imputed’ gene expression with the phenotype under investigation to identify genes involved in the etiology of the phenotype. Genetically regulated gene expression is estimated using whole-genome tissue-dependent prediction models trained with reference transcriptome data sets. PrediXcan enjoys …
Citizen Science Sensor Development - Smap | Soil Moisture Active Passive, Hagop Hovhannesian
Citizen Science Sensor Development - Smap | Soil Moisture Active Passive, Hagop Hovhannesian
STAR Program Research Presentations
“Detailed monitoring of soil moisture provides a view of how our whole Earth system works.”
The Soil Moisture Active Passive (SMAP) satellite mission was launched in January 2015; its main purpose is to acquire global measurements of soil moisture. SMAP partnered with the GLOBE program (Global Learning and Observations to Benefit the Environment), which is an international program where students collect environmental variables in a scientifically methodical way. SMAP readings and maps have various uses in various fields, which include monitoring drought, predicting floods, assisting in crop productivity, and linking water, energy and carbon cycles. The goal of this project …
Mhealth Technology: Towards A New Persuasive Mobile Application For Caregivers That Addresses Motivation And Usability, Suboh M. Alkhushayni
Mhealth Technology: Towards A New Persuasive Mobile Application For Caregivers That Addresses Motivation And Usability, Suboh M. Alkhushayni
Theses and Dissertations
With the increasing use of mobile technologies and smartphones, new methods of promoting personal health have been developed. For example, there is now software for recording and tracking one's exercise activity or blood pressure. Even though there are already many of these services, the mobile health field still presents many opportunities for new research.
One apparent area of need would be software to support the efforts of caregivers for the elderly, especially those who suffer from multiple chronic conditions, such as cognitive impairment, chronic heart failure or diabetes. Very few mobile applications (apps) have been created that target caregivers of …
Incremental Phylogenetics By Repeated Insertions: An Evolutionary Tree Algorithm, Peter Revesz, Zhiqiang Li
Incremental Phylogenetics By Repeated Insertions: An Evolutionary Tree Algorithm, Peter Revesz, Zhiqiang Li
School of Computing: Faculty Publications
We introduce the idea of constructing hypothetical evolutionary trees using an incremental algorithm that inserts species one-by-one into the current evolutionary tree. The method of incremental phylogenetics by repeated insertions lead to an algorithm that can be used on DNA, RNA and amino acid sequences. According to experimental results on both synthetic and biological data, the new algorithm generates more accurate evolutionary trees than the UPGMA and the Neighbor Joining algorithms.
Analyzing Clinical Depressive Symptoms In Twitter, Amir Hossein Yazdavar, Hussein S. Al-Olimat, Tanvi Banerjee, Krishnaprasad Thirunarayan, Amit P. Sheth
Analyzing Clinical Depressive Symptoms In Twitter, Amir Hossein Yazdavar, Hussein S. Al-Olimat, Tanvi Banerjee, Krishnaprasad Thirunarayan, Amit P. Sheth
Kno.e.sis Publications
350 million people are suffering from clinical depression worldwide.
Protein Residue-Residue Contact Prediction Using Stacked Denoising Autoencoders, Joseph Bailey Luttrell Iv
Protein Residue-Residue Contact Prediction Using Stacked Denoising Autoencoders, Joseph Bailey Luttrell Iv
Honors Theses
Protein residue-residue contact prediction is one of many areas of bioinformatics research that aims to assist researchers in the discovery of structural features of proteins. Predicting the existence of such structural features can provide a starting point for studying the tertiary structures of proteins. This has the potential to be useful in applications such as drug design where tertiary structure predictions may play an important role in approximating the interactions between drugs and their targets without expending the monetary resources necessary for preliminary experimentation. Here, four different methods involving deep learning, support vector machines (SVMs), and direct coupling analysis were …
Use Of Clustering Techniques For Protein Domain Analysis, Eric Rodene
Use Of Clustering Techniques For Protein Domain Analysis, Eric Rodene
Department of Computer Science and Engineering: Dissertations, Theses, and Student Research
Next-generation sequencing has allowed many new protein sequences to be identified. However, this expansion of sequence data limits the ability to determine the structure and function of most of these newly-identified proteins. Inferring the function and relationships between proteins is possible with traditional alignment-based phylogeny. However, this requires at least one shared subsequence. Without such a subsequence, no meaningful alignments between the protein sequences are possible. The entire protein set (or proteome) of an organism contains many unrelated proteins. At this level, the necessary similarity does not occur. Therefore, an alternative method of understanding relationships within diverse sets of proteins …
Comparing The Fieldscout Greenindex+ Chlorophyll Sensing App To The Minolta Spad Meter, Jessica D. Pille, John E. Sawyer, Daniel W. Barker
Comparing The Fieldscout Greenindex+ Chlorophyll Sensing App To The Minolta Spad Meter, Jessica D. Pille, John E. Sawyer, Daniel W. Barker
John E. Sawyer
With the improvement of mobile computing, the company Spectrum Technologies, Inc. has developed a precision Ag App which adapts an iPod, iPad, or iPhone camera to select for specific wavelengths of light from a corn leaf (Zea mays L.) in comparison to accompanying board for light/color comparison. The App computes a Dark Green Color Index (DGCI), indicating leaf greenness, which relates to the amount of chlorophyll and thus, indirectly, leaf nitrogen (N) content. The question posed for this study is: How accurate and convenient is the App compared to a proven technology, the Minolta 502 Soil-Plant Analysis Development (SPAD) meter; …
Ciliate Codon Translator Program Manual, Quentin D. Altemose
Ciliate Codon Translator Program Manual, Quentin D. Altemose
Mathematics Summer Fellows
Understanding the evolutionary history of organisms allows us to better comprehend selective pressures and their effects on larger populations. In our study, we focused on analyzing the DNA of ciliate groups, which are single celled protozoans characterized by the presence of cilia on their outer membrane. We utilized the DNA of the organisms to analyze the changes in population genotype over time. We tested existing evolutionary models (designed to represent natural genetic variation over time in populations) against our data to identify the model with the best fit and likelihood. From the DNA and the evolutionary model with the highest …
A Dynamic Run-Profile Energy-Aware Approach For Scheduling Computationally Intensive Bioinformatics Applications, Sachin Pawaskar, Hesham Ali
A Dynamic Run-Profile Energy-Aware Approach For Scheduling Computationally Intensive Bioinformatics Applications, Sachin Pawaskar, Hesham Ali
Computer Science Faculty Proceedings & Presentations
High Performance Computing (HPC) resources are housed in large datacenters, which consume exorbitant amounts of energy and are quickly demanding attention from businesses as they result in high operating costs. On the other hand HPC environments have been very useful to researchers in many emerging areas in life sciences such as Bioinformatics and Medical Informatics. In an earlier work, we introduced a dynamic model for energy aware scheduling (EAS) in a HPC environment; the model is domain agnostic and incorporates both the deadline parameter as well as energy parameters for computationally intensive applications. Our proposed EAS model incorporates 2-phases. In …
What Motivates High School Students To Take Precautions Against The Spread Of Influenza? A Data Science Approach To Latent Modeling Of Compliance With Preventative Practice, William L. Romine, Tanvi Banerjee, William R. Folk, Lloyd H. Barrow
What Motivates High School Students To Take Precautions Against The Spread Of Influenza? A Data Science Approach To Latent Modeling Of Compliance With Preventative Practice, William L. Romine, Tanvi Banerjee, William R. Folk, Lloyd H. Barrow
Kno.e.sis Publications
– This study focuses on a central question: What key behavioral factors influence high school students’ compliance with preventative measures against the transmission of influenza? We use multilevel logistic regression to equate logit measures for eight precautions to students’ latent compliance levels on a common scale. Using linear regression, we explore the efficacy of knowledge of influenza, affective perceptions about influenza and its prevention, prior illness, and gender in predicting compliance. Hand washing and respiratory etiquette are the easiest precautions for students, and hand sanitizer use and keeping the hands away from the face are the most difficult. Perceptions of …