Life Sciences Commons^™

A Deeper Understanding Of Noise Effects On Cetaceans, Jason N. Bruck

Faculty Publications

Recent research with cetaceans under human care is illuminating just how dolphins are affected by human-made noise both in terms of their ability to cooperate as well as their ability to habituate to such noise. This research is providing granular detail to regulators assessing the problems associated with anthropogenic effects and is highlighting a role for behavior/cognition research in conservation.

A Quieter Ocean: Experimentally Derived Differences In Attentive Responses Of Tursiops Truncatus To Anthropogenic Noise Playbacks Before And During The Covid-19-Related Anthropause, Paige E. Stevens, Veda Allen, Jason N. Bruck

Faculty Publications

The effects of anthropogenic noise continue to threaten marine fauna, yet the impacts of human-produced sound on the broad aspects of cognition in marine mammals remain relatively understudied. The shutdown of non-essential activities due to the COVID-19-related anthropause created an opportunity to determine if reducing levels of oceanic anthropogenic noise on cetaceans affected processes of sensitization and habituation for common human-made sounds in an experimental setting. Dolphins at Dolphin Quest Bermuda were presented with three noises related to human activities (cruise ship, personal watercraft, and Navy low-frequency active sonar) both in 2018 and again during the anthropause in 2021 via …

A Molecular Chemodosimeter To Probe “Closed Shell” Ions In Kidney Cells, Rashid Mia

Faculty Publications

Two quinidine-functionalized coumarin molecular probes have been synthesized and have been found to bind metal cations (Cd2+, Co2+, Cu2+, Fe2+, Hg2+, Ni2+, and Zn2+) with high affinity in organic–aqueous media (DMSO–HEPES). The chemodosimeters coordinate with the Zn2+ ions in a two-to-one ratio (molecular probe : Zn2+) with a log β of 10.0 M−2. Upon the addition of the closed-shell metal ions studied, a fluorescence turn-on via an excimer formation is seen at 542 nm due to the quinaldine moiety adopting a syn arrangement when coordinated to the metal Zn2+ ions. Confocal microscopy monitored free Zn2+ ions in the Human Embryonic …

Cross-Modal Perception Of Identity By Sound And Taste In Bottlenose Dolphins, Jason N. Bruck, Sam F. Walmsley, Vincent M. Janik

Faculty Publications

While studies have demonstrated concept formation in animals, only humans are known to label concepts to use them in mental simulations or predictions. To investigate whether other animals use labels comparably, we studied cross-modal, individual recognition in bottlenose dolphins (Tursiops truncatus) that use signature whistles as labels for conspecifics in their own communication. First, we tested whether dolphins could use gustatory stimuli and found that they could distinguish between water and urine samples, as well as between urine from familiar and unfamiliar individuals. Then, we paired playbacks of signature whistles of known animals with urine samples from either the same …

Evolutionary Analyses Of Visual Opsin Genes In Frogs And Toads: Diversity, Duplication, And Positive Selection, Ryan K. Schott, Leah Perez, Matthew Kwiatkowski, Vance Imhoff, Jennifer M. Gumm

Faculty Publications

Among major vertebrate groups, anurans (frogs and toads) are understudied with regard to their visual systems, and little is known about variation among species that differ in ecology. We sampled North American anurans representing diverse evolutionary and life histories that likely possess visual systems adapted to meet different ecological needs. Using standard molecular techniques, visual opsin genes, which encode the protein component of visual pigments, were obtained from anuran retinas. Additionally, we extracted the visual opsins from publicly available genome and transcriptome assemblies, further increasing the phylogenetic and ecological diversity of our dataset to 33 species in total. We found …

Cetacean Acousticwelfare In Wild And Managed-Care Settings: Gaps And Opportunities, Paige E. Stevens, Heather Hill, Jason N. Bruck

Faculty Publications

Cetaceans are potentially at risk of poor welfare due to the animals’ natural reliance on sound and the persistent nature of anthropogenic noise, especially in the wild. Industrial, commercial, and recreational human activity has expanded across the seas, resulting in a propagation of sound with varying frequency characteristics. In many countries, current regulations are based on the potential to induce hearing loss; however, a more nuanced approach is needed when shaping regulations, due to other non-hearing loss effects including activation of the stress response, acoustic masking, frequency shifts, alterations in behavior, and decreased foraging. Cetaceans in managedcare settings share the …

Propranolol Sensitizes Vascular Sarcoma Cells To Doxorubicin By Altering Lysosomal Drug Sequestration And Drug Efflux, Jhuma Saha, Jong Hyuk Kim, Clarissa N. Amaya, Caleb M. Witcher, Ali Khammanivong, Derek M. Korpela, David R. Brown, Josephine Taylor, Brad A. Bryan, Erin B. Dickerson

Faculty Publications

Angiosarcoma is a rare cancer of blood vessel–forming cells with a high patient mortality and few treatment options. Although chemotherapy often produces initial clinical responses, outcomes remain poor, largely due to the development of drug resistance. We previously identified a subset of doxorubicin-resistant cells in human angiosarcoma and canine hemangiosarcoma cell lines that exhibit high lysosomal accumulation of doxorubicin. Hydrophobic, weak base chemotherapeutics, like doxorubicin, are known to sequester within lysosomes, promoting resistance by limiting drug accessibility to cellular targets. Drug synergy between the beta adrenergic receptor (β-AR) antagonist, propranolol, and multiple chemotherapeutics has been documented in vitro, and …

Bias And Misrepresentation Of Science Undermines Productive Discourse On Animal Welfare Policy: A Case Study, Kelly Jaakkola, Jason N. Bruck, Richard C. Connor, Stephen H. Montgomery, Stephanie L. King

Faculty Publications

Reliable scientific knowledge is crucial for informing legislative, regulatory, and policy decisions in a variety of areas. To that end, scientific reviews of topical issues can be invaluable tools for informing productive discourse and decision-making, assuming these reviews represent the target body of scientific knowledge as completely, accurately, and objectively as possible. Unfortunately, not all reviews live up to this standard. As a case in point, Marino et al.’s review regarding the welfare of killer whales in captivity contains methodological flaws and misrepresentations of the scientific literature, including problematic referencing, overinterpretation of the data, misleading word choice, and biased argumentation. …

Can The Fut 2 Gene Variant Have An Effect On The Body Weight Of Patients Undergoing Bariatric Surgery? - Preliminary, Exploratory Study, Natalia Komorniak, Alexandra Martynova-Van Kley, Armen Nalian, Wiktoria Wardziukiewicz, Karolina Skoniecznaa-Zydecka, Daniel Styburski, Joanna Palma, Bartosz Kowalewski, Krzysztof Kaseja, Ewa Stachowska

Faculty Publications

Background: The FUT2 gene (Se gene) encoding the enzyme α-1,2-L-fucosyltransferase 2 seems to have a significant effect on the number and type of bacteria colonizing the intestines. Methods: In a group of 19 patients after bariatric surgery, the polymorphism (rs601338) of FUT2 gene was analyzed in combination with body mass reduction, intestinal microbiome (16S RNA sequencing), and short chain fatty acids (SCFA) measurements in stools. Results: Among the secretors (Se/Se polymorphism of the FUT2 gene rs601338, carriers of GG variant), correlations between waist-hip ratio (WHR) and propionate content and an increase in Prevotella, Escherichia, Shigella, and Bacteroides were observed. On …

A Comparison Of The Trojan Y Chromosome Strategy To Harvesting Models For Eradication Of Non-Native Species, Jingjing Lyu, Pamela J. Schofield, Kristen M. Reaver, Matthew Beauregard, Rana D. Parshad

Faculty Publications

The Trojan Y Chromosome Strategy (TYC) is a promising eradication method for biological control of non-native species. The strategy works by manipulating the sex ratio of a population through the introduction of supermales that guarantee male offspring. In the current manuscript, we compare the TYC method with a pure harvesting strategy. We also analyze a hybrid harvesting model that mirrors the TYC strategy. The dynamic analysis leads to results on stability of solutions and bifurcations of the model. Several conclusions about the different strategies are established via optimal control methods. In particular, the results affirm that either a pure harvesting …

Large And Small Data Blow-Up Solutions In The Trojan Y Chromosome Model, Rana D. Parshad, Matthew Beauregard, Eric M. Takyi, Thomas Griffin, Landrey Bobo

Faculty Publications

The Trojan Y Chromosome Strategy (TYC) is an extremely well investigated biological control method for controlling invasive populations with an XX-XY sex determinism. In [35, 36] various dynamical properties of the system are analyzed, including well posedness, boundedness of solutions, and conditions for extinction or recovery. These results are derived under the assumption of positive solutions. In the current manuscript, we show that if the introduction rate of trojan fish is zero, under certain large data assumptions, negative solutions are possible for the male population, which in turn can lead to finite time blow-up in the female and male populations. …

Diversity And Shifts Of The Bacterial Community Associated With Baikal Sponge Mass Mortalities, Sergei Belikov, Natalia Belkova, Tatiana Butina, Lubov Chernogor, Alexandra Martynova-Van Kley, Armen Nalian, Colin Rorex, Igor Khanaev, Olga Maikova, Sergey Feranchuk

Faculty Publications

The disease of freshwater sponges was first discovered in 2011, when pink samples were found in the Central Basin of Lake Baikal. Subsequently, the visible signs of the disease have changed, and now sponges appear with various symptoms of damage to the body, such as discoloration, tissue necrosis, the formation of brown patches and dirty-purple biofilms on some branches. These signs of the disease are accompanied by the mass death of sponges. We identified differences in microbiomes by sequencing 16S rRNA genes and found changes in the consortium of microorganisms of freshwater Baikal sponges. We found that the observed imbalance …

Structural And Functional Determinants Of Rodent And Human Surfactant Protein A: A Synthesis Of Binding And Computational Data, Armen Nalian, Todd M. Umstead, Ching-Hui Yang, Patricia Silveyra, Neal J. Thomas, Joanna Floros, Francis X. Mccormack, Zissis C. Chroneos

Faculty Publications

Surfactant protein A (SP-A) provides surfactant stability, first line host defense, and lung homeostasis by binding surfactant phospholipids, pathogens, alveolar macrophages (AMs), and epithelial cells. Non-primates express one SP-A protein whereas humans express two: SP-A1 and SP-A2 with core intra- and inter-species differences in the collagen-like domain. Here, we used macrophages and solid phase binding assays to discern structural correlates of rat (r) and human (h) SP-A function. Binding assays using recombinant rSP-A expressed in insect cells showed that lack of proline hydroxylation, truncations of amino-terminal oligomerization domains, and site-directed serine (S) or alanine (A) mutagenesis of cysteine 6 (C6S), …

Whole Leaf Photosynthetic Light Response In A Carnivorous Plant Species Sarracenia Alata, K. A. Hopkins, Dennis A. Gravatt

Faculty Publications

Photosynthetic light-response curves of mature leaves of Sarracenia alata were generated and characterized using a custom built chamber (2.79 L) capable of accommodating whole leaves, or pitchers, coupled to a LI-COR 6400XT Portable Photosynthesis System. In addition to generating light-response curves, chlorophyll content, leaf area, and dry mass measurements were obtained for each pitcher. Gas-exchange variables from the light-response curves are reported on a leaf area and dry mass basis. Calculated light-saturated photosynthesis was found to be 2.09 µmol(CO₂) m^-2 s^-1 with a light-saturation point of 315.14 µmol(photon) m^-2 s^-1, light-compensation point of …

Arachidin-1 And Arachidin-3 Modulation Of Rotavirus-Infected Ma104 Cells, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah N. Lockwood, Macie N. Mattila, Stormey B. Wisdom, Luanna L. Saade Ferreira, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball, Rebecca D. Parr

Faculty Publications

Rotavirus (RV) causes severe life-threatening diarrhea in young children and immunocompromised individuals. There are several licensed attenuated vaccines for young children, but there are no vaccines or antiviral therapeutics for immunocompromised patients of any age. Previously, our laboratory demonstrated that arachidin 1 (A1) and arachidin 3 (A3) decreases the number of infectious simian RV particles and RV non-structural protein 4 (NSP4) in a human intestinal cell line which suggests effects on RV replication. This study examined the effects of the arachidins on the human RV (Wa)-infected African green monkey kidney cell line, MA104. The addition of either A1 or A3 …

A Time Course Study Of Rotavirus-Infected Intestinal Cells Treated With Stilbenoids And The Regulation Of Apoptosis, Rebecca D. Parr, Caleb M. Witcher, Rebekah Napier-Jameson, Hannah L. Wilson, Stormey Wisdom, Macie N. Mattila, Essence B. Strange, Josephine Taylor, Beatrice Clack, Fabricio Medina-Bolivar, Judith M. Ball

Faculty Publications

This is a time course study of virus –host interactions that are modified with the addition of two small natural products. They appear to effect virus replication and the host response to the infection.

The Roles Of Inter- And Intra-Sexual Selection In Behavioral Isolation Between Native And Invasive Pupfishes, Cory Becher, Jennifer M. Gumm

Faculty Publications

Male-male competition and female mate choice may both play important roles in driving and maintaining reproductive isolation between species. When previously allopatric species come into secondary contact with each other due to introductions, they provide an opportunity to evaluate the identity and strength of reproductive isolating mechanisms. If reproductive isolation is not maintained, hybridization may occur. We examined how reproductive isolating mechanisms mediate hybridization between endemic populations of the Red River pupfish Cyprinodon rubrofluviatilis and the recently introduced sheepshead minnow C. variegatus. In lab-based dominance trials, males of both species won the same number of competitions. However, male C. rubrofluviatilis …

The Effects Of Ultraviolet Light On Anthocyanin Accumulation In The Adventitious Roots Of Sedum Wrightii (Crassulaceae), Lila D. Kerr, Dennis A. Gravatt, Robert J. Wiggers

Faculty Publications

Several studies have supported the idea that anthocyanin accumulation may be a possible protection mechanism in plants against DNA damage caused by ultraviolet radiation (UV). This study explored the accumulation of anthocyanins in the adventitious root tips of Sedum wrightii using the following treatments: UVA, UVA+low UVB, and UVA+high UVB. Following exposure to UV radiation, samples were analyzed for anthocyanin accumulation using an ethanol extraction procedure. Using ELISA, additional root samples were analyzed for indicators of DNA damage: cyclobutane pyrimidine dimers (CPDs) and pyrimidine (6-4) pyrimidone dimers (6-4 PPs). The anthocyanin concentrations were significantly higher in the UVA + high …

Utilization Of Pyrosequencing To Monitor The Microbiome Dynamics Of Probiotic Treated Poultry (Gallus Gallus Domesticus) During Downstream Poultry Processing, Vamsy Priya Guttala, Enrique G. Medrano, Joey Bray, Beatrice Clack

Faculty Publications

Antibiotic growth promoters that have been historically employed to control pathogens and increase the rate of animal development for human consumption are currently banned in many countries. Probiotics have been proposed as an alternative to control pathogenic bacteria. Traditional culture methods typically used to monitor probiotic effects on pathogens possess significant limitations such as a lack in sensitivity to detect fastidious and non-culturable bacteria, and are both time consuming and costly. Here, we tested next generation pyrosequencing technology as a streamline and economical method to monitor the effects of a probiotic on microbial communities in juvenile poultry (Gallus gallus …

Spatial Distribution Of Earthworms In An East Texas Forest Ecosystem, Melissa A. Bozarth, Kenneth W. Farrish, George A. Damoff, James Van Kley, J. Leon Young

Faculty Publications

Earthworms were collected and identified in different ecological habitats of the Stephen F. Austin Experimental Forest (SFAEF) in the Piney Woods Ecoregion (PWE) of Texas. Earthworm spatial distribution data were collected over four distinct ecological habitats with a range of soil conditions and vegetative cover. A total of 128 sampling plots were surveyed in two different, broadly defined locations (mesic slope = 68 plots, dry-mesic upland = 60 plots). Using multivariate classification/ordination (TWINSPAN) and detrended correspondence analysis (DCA) of overstory vegetation data, these two locations were further divided into four distinct habitats: dry-mesic mixed upland, transitional zone, mesic slope and …

Road-Edge Effects On Herpetofauna In A Lowland Amazonian Rainforest, Ross J. Maynard, Nathalie C. Aall, Daniel Saenz, Paul S. Hamilton, Matthew Kwiatkowski

Faculty Publications

The impact of roads on the flora and fauna of Neotropical rainforest is perhaps the single biggest driver of habitat modification and population declines in these ecosystems. We investigated the road-edge effect of a low-use dirt road on amphibian and reptile abundance, diversity, and composition within adjacent lowland Amazonian rainforest at San José de Payamino, Ecuador. The road has been closed to vehicle traffic since its construction in 2010. Thus, effects from vehicle mortality, vehicle-related pollution, and road noise were not confounding factors. Herpetofauna were surveyed using both visual encounter surveys and drift fences with pitfall and funnel traps at …

Habitat Use And Avoidance By Foraging Red-Cockaded Woodpeckers In East Texas, John N. Macey, Brent Burt, Daniel Saenz, Richard N. Conner

Faculty Publications

Picoides borealis (Red-cockaded Woodpecker) is an endangered bird endemic to the Pinus (pine) ecosystems of the southeastern US. Mature pine savannahs with a minimal midstory and lush herbaceous groundcover represent high-quality habitat. This study examines the foraging-habitat patterns of Red-cockaded Woodpeckers in East Texas. We present a logistic regression model that best differentiates between foraged and non-foraged habitat. Increases in hardwood-midstory basal area have the greatest negative impact on the probability of Red-cockaded Woodpeckers selecting a habitat patch for foraging. Five additional variables negatively impact foraging probability: shrub height, diameter at breast height (DBH) of pine midstory, canopy closure, density …

Morphologic And Taxonomic Analysis Of The Weedy And Cultivated Amaranthus Hybridus Species Complex, Dinesh Adhikary, Donald B. Pratt

Faculty Publications

The hybridus species complex of the genus Amaranthus is a group of weedy and cultivated plants from the New World that are considered difficult to identify. Classification schemes have varied between a single species approach, Amaranthus hybridus s.l., and a five species approach that recognizes the widespread weedy A. hybridus s.s., the South American endemic A. quitensis, and the three cultivated taxa (A. hypochondriacus, A. cruentus, and A. caudatus) as distinct species. The goals of this study were to analyze patterns of floral variation within the species complex and to determine distinguishing morphological features of …

Effects Of Urbanization On The Occurrence Of Batrachochytrium Dendrobatidis: Do Urban Environments Provide Refuge From The Amphibian Chytrid Fungus?, Daniel Saenz, Taylor L. Hall, Matthew Kwiatkowski

Faculty Publications

Batrachochytrium dendrobatidis (Bd) is a widespread pathogenic fungus that is known to cause the disease, chytridiomycosis, which can be lethal to many amphibians. We compared occurrence rates on spring peepers (Pseudacris crucifer) in urban and forested breeding sites in eastern Texas, USA. All study sites were at approximately the same latitude and altitude, and samples were collected at the same time of year to isolate differences in Bd infection rates between habitat types. We found significant differences (p<0.001) in the occurrence of Bd between habitats; with dramatically lower rates of occurrence at urban sites (19.5 %), compared to forested sites (62.9 …

Relationships Between The Weedy (Amaranthaceae) And The Grain Amaranths [Abstract], Kiel D. Kielinski, Felix Jimenez, Eric N. Jellen, Peter J. Maughan, Scott M. Smith, Donald B. Pratt

Faculty Publications

No abstract provided.

Intrinsic And Extrinsic Factors Influence Expression Of Defensive Behavior In Plains Hog‐Nosed Snakes [Abstract], Andrew M. Durso, Stephen J. Mullin

Faculty Publications

No abstract provided.

Assessing Multiple Endpoints Of Atrazine Ingestion On Gravid Northern Watersnakes (Nerodia Sipedon) And Their Offspring [Abstract], L. A. Neuman-Lee, K. F. Gaines, K. A. Baumgartner, J. R. Voorhees, J. M. Novak, Stephen J. Mullin

Faculty Publications

No abstract provided.

Cloning, Expression, Sequence Analysis And Homology Modeling Of The Prolyl Endoprotease From Eurygaster Integriceps Puton, Ravi Chandra Yandamuri, Ranjeeta Gautam, Charles Darkoh, Vanitha Dareddy, Mustapha El-Bouhssini, Beatrice A. Clack

Faculty Publications

Eurygaster integriceps Puton, commonly known as sunn pest, is a major pest of wheat in Northern Africa, the Middle East and Eastern Europe. This insect injects a prolyl endoprotease into the wheat, destroying the gluten. The purpose of this study was to clone the full length cDNA of the sunn pest prolyl endoprotease (spPEP) for expression in E. coli and to compare the amino acid sequence of the enzyme to other known PEPs in both phylogeny and potential tertiary structure. Sequence analysis shows that the 5ꞌ UTR contains several putative transcription factor binding sites for transcription factors known to be …

Effect Of Origanum Chemotypes On Broiler Intestinal Bacteria, Liliana Betancourt, Fernando Rodriguez, Vienvilay Phandanouvong, Claudia Ariza-Nieto, Michael Hume, David Nisbet, German Afanador-Tellez, Alexandra Martynova-Van Kley, Armen Nalian

Faculty Publications

Essential oils have been proposed as alternatives to antibiotic use in food animal production. This study evaluated 3 chemotypes of the Origanum genus, containing varying amounts of secondary metabolites carvacrol, thymol, and sabinene, in the broiler chicken diet. Aerial parts of Origanum vulgare L. (OL), O. vulgare L. ssp. hirtum (OH), and O. majorana (OM) were collected from a greenhouse located in the high altitude Sabana de Bogotá (Savanna of Bogotá) and O. vulgare L. ssp. hirtum (OG) produced and ground in Greece. Oregano essential oils (OEO) from these plants were obtained by steam distillation and analyzed by gas chromatography …

Mutational Analysis Of The Rotavirus Nsp4 Enterotoxic Domain That Binds To Caveolin-1, Judith M. Ball, Megan E. Schroeder, Cecelia V. Williams, Friedhelm Schroeder, Rebecca D. Parr

Faculty Publications

Background: Rotavirus (RV) nonstructural protein 4 (NSP4) is the first described viral enterotoxin, which induces early secretory diarrhea in neonatal rodents. Our previous data show a direct interaction between RV NSP4 and the structural protein of caveolae, caveolin-1 (cav-1), in yeast and mammalian cells. The binding site of cav-1 mapped to the NSP4 amphipathic helix, and led us to examine which helical face was responsible for the interaction.

Methods: A panel of NSP4 mutants were prepared and tested for binding to cav-1 by yeast two hybrid and direct binding assays. The charged residues of the NSP4 amphipathic helix were changed …