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Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha
Effect Of Picloram, Additives And Plant Growth Regulators On Somatic Embryogenesis Of Phyla Nodiflora (L.) Greene, Rosna Binti Mat Taha
Rosna Binti Mat Taha
The present study describes the plant regeneration via somatic embryogenesis in suspension culture derived from the leaf and stem explants of Phyla nodiflora. The medium type, plant growth regulators, complex extract (coconut milk and malt extract) and anti-oxidant (activated charcoal, ascorbic acid, Polyvinylpyrrolidone and citric acid) markedly influenced the embryo regeneration of P. nodiflora. MS with 2,4-D and activated charcoal (10 mg/L) gave the highest stimulation of embryogenic callus growth. Optimized callus was transfered into suspension culture, which showed the globular, heart shaped embryos in MS with 2,4-D + BA + picloram (0.1 mg/L), coconut milk (10 ml/L), citric acid …