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Microarray-Based Method For Detection Of Unknown Genetic Modifications, Torstein Tengs
Microarray-Based Method For Detection Of Unknown Genetic Modifications, Torstein Tengs
Dr. Torstein Tengs
Background
Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed a strategy for detection and characterization of unknown genetic modifications and we present a proof of concept for this method using Arabidopsis thaliana and Oryza sativa (rice). The approach relies on direct hybridization of total genomic DNA to high density microarrays designed to have probes tiled throughout a set of reference sequences.
Results
We show that by using arrays with 25 basepair probes covering both …