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Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers
Regulation Of Liver Mitochondrial Metabolism During Hibernation By Post-Translational Modification, Katherine E. Mathers
Electronic Thesis and Dissertation Repository
Hibernation, characterized by a seasonal reduction in metabolism and body temperature, allows animals to conserve energy when environmental conditions (e.g. temperature, food availability) are unfavourable. During hibernation, small mammals such as the 13-lined ground squirrel (Ictidomys tridecemlineatus) cycle between two distinct metabolic states: torpor, where metabolic rate is suppressed by >95% and body temperature falls to ~5 °C, and interbout euthermia (IBE), where metabolic rate and body temperature rapidly increase and are maintained at euthermic levels several hours. Suppression of metabolism during entrance into torpor is paralleled by rapid suppression of liver mitochondrial metabolism. In my thesis, I …
Changes In The Mitochondrial Phosphoproteome During Mammalian Hibernation, Dillon J. Chung
Changes In The Mitochondrial Phosphoproteome During Mammalian Hibernation, Dillon J. Chung
Electronic Thesis and Dissertation Repository
Hibernation in mammals involves a suppression of metabolic rate thereby conserving energy. Many hibernation studies focus on the mitochondria due to their energy-producing role. I hypothesized that this pattern of rapid suppression of liver mitochondrial metabolism with gradual reversal, was related to changes in the phosphorylation state of Ictidomys tridecemlineatus’ mitochondrial proteins in different stages of torpor bouts. Phosphorylation of threonine and serine amino-acid residues (unique to 49, 41 and 47, 38 kDa proteins respectively), detected using immunoblotting, changes seasonally (threonine: summer active > winter, serine: summer active > torpor), whereas no seasonal difference was observed in phosphotyrosine residues. Changes in …