Life Sciences Commons^™

Sociality Increases Juvenile Survival After A Catastrophic Event In The Feral Horse (Equus Caballus), Cassandra M.V. Nuñez, James S. Adelman, Daniel I. Rubenstein

Cassandra M.V. Nuñez

In several social species, adult associations have been linked to individual fitness. Less is known about offspring associations and the mechanisms by which they may influence fitness. We investigate the effects of sociality on juvenile survival in feral horses (Equus caballus). We use foal degree (number of associates) and foal weight (number of interactions) to assess sociality’s importance to foal survival of a catastrophic event, the gathering and removal of 40% of the horse population. We found that 1) foal degree was a better predictor of foal survival than was foal weight; 2) following the gather, foals with access to …

Repeated Stressors In Adulthood Increase The Rate Of Biological Ageing, Michaela Hau, Mark F. Haussmann, Timothy J. Greives, Christa Matlack, David Costantini, Michael Quetting, James S. Adelman, Ana C. Miranda, Jesko Partecke

James S. Adelman

Individuals of the same age can differ substantially in the degree to which they have accumulated tissue damage, akin to bodily wear and tear, from past experiences. This accumulated tissue damage reflects the individual’s biological age and may better predict physiological and behavioural performance than the individual‘s chronological age. However, at present it remains unclear how to reliably assess biological age in individual wild vertebrates. We exposed hand-raised adult Eurasian blackbirds (Turdus merula) to a combination of repeated immune and disturbance stressors for over one year to determine the effects of chronic stress on potential biomarkers of biological ageing including …

A Free-Ranging, Feral Mare Equus Caballus Affords Similar Maternal Care To Her Genetic And Adopted Offspring, Cassandra M.V. Nuñez, James S. Adelman, Daniel I. Rubenstein

Cassandra M.V. Nuñez

Adoption of nongenetic offspring occurs in a variety of species but is rare in equids. We report a case of adoption by a free-ranging, feral mare Equus caballus and compare the maternal care received by her genetic offspring (born 1995) to that of her adopted offspring (born 1996) for the first 30 weeks of development. We compare five measures of care: (1) total time spent suckling, (2) mare aggression during suckling, (3) number of mare-terminated suckling bouts, (4) contact maintenance, and (5) mare-foal distance. For most behaviors, we detected no difference in the mare’s treatment of the two foals; however, …

House Finch Populations Differ In Early Inflammatory Signaling And Pathogen Tolerance At The Peak Of Mycoplasma Gallisepticum Infection, James S. Adelman, Laila Kirkpatrick, Jessica L. Grodio, Dana M. Hawley

James S. Adelman

Host individuals and populations often vary in their responses to infection, with direct consequences for pathogen spread and evolution. While considerable work has focused on the mechanisms underlying differences in resistance—the ability to kill pathogens— we know little about the mechanisms underlying tolerance— the ability to minimize fitness losses per unit pathogen. Here, we examine patterns and mechanisms of tolerance between two populations of house finches (Haemorhous [formerly Carpodacus] mexicanus) with different histories with the bacterial pathogen Mycoplasma gallisepticum (MG). After infection in a common environment, we assessed two metrics of pathology, mass loss and eye lesion severity, as proxies …

Method And System For Lactose-Free Or Lactose-Reduced Milk And Associated Products, Production Thereof, And Associated Processes, Joseph H. Hotchkiss, Joey Talbert

Joey Talbert

A system and method capable of hydrolyzing lactose, where the system includes a support formed from a functionalized hydrophobic polymer that is covalently linked to a hydrophilic molecule covalently that is, in turn, covalently linked to an enzyme such as lactose. The method includes the steps of functionalizing a hydrophobic polymer support, covalently linking a hydrophilic molecule to said functionalized polymer support, and covalently linking an enzyme such as lactase to said hydrophilic molecule. The system and method generally relate to the field of food science and engineering and, more particularly to dairy-based food products and their production including solutions …

Twin-Screw Extrusion Processing Of Distillers Dried Grains With Solubles (Ddgs)-Based Yellow Perch (Perca Flavescens) Feeds, Ferouz Y. Ayadi, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, Michael L. Brown

Kurt A. Rosentrater

Increases in global aquaculture production, compounded with limited availabilities of fish meal for fish feed, has created the need for alternative protein sources. Twin-screw extrusion studies were performed to investigate the production of nutritionally balanced feeds for juvenile yellow perch (Perca flavescens). Five isocaloric (~3.06 kcal/g) ingredient blends, adjusted to a target protein content of 36.7% db, were formulated with 0%, 10%, 20%, 30%, and 40% distillers dried grains with solubles (DDGS) at an initial moisture content of 5–7% db, with appropriate amounts of fish meal, fish oil, whole wheat flour, corn gluten meal, and vitamin and mineral premixes. During …

Nutritional Composition And Use Of Common Carp Muscle In Yellow Perch Diets, Travis W. Schaeffer, Matthew J. Hennen, Michael L. Brown, Kurt A. Rosentrater

Kurt A. Rosentrater

High market demand for marine fish meals coupled with increasing costs and questionable sustainability of wild stocks have led researchers to investigate a variety of alternative plant and animal protein sources for aquaculture feeds. Our objective was to evaluate the use of common carp Cyprinus carpio, a locally abundant, nonnative fish species, to offset the cost of marine fish meal in fish feed. We completed analyses of common carp whole muscle, formulated diets containing combinations of carp and Gulf menhaden Brevoortia patronus fish meal, and then evaluated test diets in a feeding trial with yellow perch Perca flavescens. Composition (dry …

Alternative Protein Sources For Aquaculture Feeds, Ferouz Y. Ayadi, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

With a global seafood production of about 51.7 million ton in 2006 and an annual growth rate of 6.9% from 1970-2006, aquaculture is one of the fastest growing sectors in the food industry. Feed represents 40-70% of operating costs for aquaculture operations. Fish diets typically contain between 20 and 55% crude protein, depending on the fish species. High quantities of fish meal are commonly used in these feeds to supply fish with essential proteins, amino acids and fatty acids. However, during the last 3 years prices for fish meal have remained above $1000 ton-1 and have even approached $2000 ton-1 …

Extruded Aquafeeds Containing Distillers Dried Grains With Solubles: Effects On Extrudate Properties And Processing Behaviour, Kamal Mjoun, Kurt A. Rosentrater

Kurt A. Rosentrater

BACKGROUND: The tremendous supply and low cost of distillers dried grains with solubles (DDGS) make it an attractive feedstuff for aquaculture diets. Also, several studies have shown that DDGS can be successfully fed to various finfish. The objective of this study was to evaluate the effects of inclusion rate of DDGS (0, 250, 500 g kg−1), feed moisture content (350, 450 g kg−1) and die opening area (die A = 18.85 mm2, die B = 3988.45 mm2) on the properties of the extrudates and on processing behaviour using a single-screw extruder.

RESULTS: Increasing the inclusion rate of DDGS resulted in …

Effects Of Conditioner Steam, Extruder Water And Screw Speed On Physical Properties Of Ddgs-Based Extrudates In Twin-Screw Extrusion, Parisa Fallahi, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, Mehmet Tulbek

Kurt A. Rosentrater

A fractional factorial design with a replicated central composite point was used to investigate the effects of extrusion processing on physical properties of DDGS–based aquafeeds using a twin screw extruder. Extrusion cooking trials were performed using an ingredient blend for Nile tilapia, with 2 levels of screw speed (350 and 450 rpm), 2 levels of extruder water (0.236 and 0.302 kg/min) and 2 levels of conditioner steam (0.1 and 0.15 kg/min). The central point was 400 rpm screw speed, 0.271 kg/min extruder water, and 0.12 kg/min conditioner steam. Effects of these processing conditions on extrudate characteristics were extensively analyzed, and …

Single-Screw Extrusion Modeling Effects On Extrusion Processing Parameters And Physical Properties Of Ddgs-Based Nile Tilapia ( Oreochromis Niloticus ) Feeds, Ferouz Y. Ayadi, Parisa Fallahi, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

A single-screw laboratory extruder was used to conduct an L 18 (2 2 X 3 6 ) Taguchi fractional factorial study of aquafeed processing. The ingredients were based on a formulation for nutritionally-balanced Nile tilapia diets containing distillers dried grains with solubles (DDGS) and soybean meal as the main protein sources, in addition to constant amounts of corn flour, whey, and fish meal. The effects of three levels of DDGS (20, 30 and 40%), soybean meal (30, 40 and 50%), ingredient moisture content (20, 30 and 40% db), screw speed (100, 150 and 200 rpm), barrel temperatures (80-100-100°C, 80-120-120°C and …

Twin-Screw Extrusion Processing Of Rainbow Trout (Oncorhynchus Mykiss) Feeds Using Various Levels Of Corn-Based Distillers Dried Grains With Solubles (Ddgs), Ferouz Y. Ayadi, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater, Michael L. Brown

Kurt A. Rosentrater

Increasing demand for seafood products and rising demand for fish meal for commercial fish feeds is driving the search for effective alternative protein sources. Twin-screw extrusion trials were conducted to study the production of nutritionally balanced feeds for rainbow trout fingerlings (Oncorhynchus mykiss). Six isocaloric (≈4.61 kcal/g) ingredient blends with a target protein content of >45% db were formulated with 0, 10, 20, 30, 40, and 50% distillers dried grains with solubles (DDGS) and other feed ingredients. The moisture contents of the diets were initially adjusted to 5–7% db, and then extruded at 250 rpm using dual 1.9 mm dies …

Effect Of Starch Sources And Protein Content On Extruded Aquaculture Feed Containing Ddgs, Sankaranandh Kannadhason, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater

Kurt A. Rosentrater

A 3 × 3 × 3 completely randomized design was used to investigate extrusion cooking behavior and product characteristics of distillers dried grains with solubles (DDGS), protein levels, and various starch sources in a laboratory scale single screw extruder. Cassava, corn, and potato starches with varying levels of DDGS (20%, 30%, and 40% wet basis (wb)) were extruded with three different proportions of protein levels (28%, 30%, and 32% wb). The extrusion cooking was performed at a constant feed moisture content of 20% wb, barrel temperature of 120 °C, and a preset screw speed of 130 rpm (13.6 rad/s). Extrudate …

Effects Of Dietary Distillers Dried Grains With Solubles And Soybean Meal On Extruded Pellet Characteristics And Growth Responses Of Juvenile Yellow Perch, Travis W. Schaeffer, Michael L. Brown, Kurt A. Rosentrater

Kurt A. Rosentrater

A 126-d feeding trial was performed to investigate graded combinations of distillers dried grains with solubles (DDGS) and soybean meal (SBM) in diets formulated for yellow perch Perca flavescens. Six experimental diets contained DDGS and SBM at 0 and 31.5% (dry matter basis), respectively (0/31.5 diet), 10 and 26% (10/26), 20 and 20.5% (20/20.5), 30 and 15% (30/15), 40 and 9.5% (40/9.5), and 50 and 4% (50/4) to obtain similar levels of crude protein (mean ± SE = 30.1 ± 0.2%), crude lipid (16.7 ± 0.7%), and digestible energy (13.5 ± 0.2 kJ/g). Fourteen fish (initial individual weight = 19.1 …

Utilization Of Diets Containing Graded Levels Of Ethanol Production Co-Products By Nile Tilapia, Travis W. Schaeffer, Michael L. Brown, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

A feeding trial was performed to investigate inclusion levels of distillers dried grains with solubles (DDGS) as a fishmeal replacement for juvenile Nile tilapia (Oreochromis niloticus). On a dry matter basis, five isocaloric [19.3 ± 0.4 kJ/g (mean ± SE)], isonitrogenous (39.1 ± 0.5% crude protein) diets were formulated to contain 17.5%, 20%, 22.5%, 25%, and 27.5% DDGS and compared against a 0% DDGS, reference diet (gross energy = 14.5 kJ/g; crude protein = 39.8%). The reference diet resulted in significantly higher body weight gain (BWG), food conversion ratio (FCR), and protein efficiency ratio (PER) than experimental diets except that …

Immunocontraception In Wild Horses (Equus Caballus) Extends Reproductive Cycling Beyond The Normal Breeding Season, Cassandra M.V. Nuñez, James S. Adelman, Daniel I. Rubenstein

Cassandra M.V. Nuñez

Although the physiological effects of immunocontraceptive treatment with porcine zona pellucida (PZP) have been well studied, little is known about PZP's effects on the scheduling of reproductive cycling. Recent behavioral research has suggested that recipients of PZP extend the receptive breeding period into what is normally the non-breeding season. To determine if this is the case, we compiled foaling data from wild horses (Equus caballus) living on Shackleford Banks, North Carolina for 4 years pre- and 8 years post-contraception management with PZP (pre-contraception, n = 65 births from 45 mares; post-contraception, n = 97 births from 46 mares). Gestation lasts …

Tilapia: Profile And Economic Importance, Kamal Mjoun, Kurt A. Rosentrater, Michael L. Brown

Kurt A. Rosentrater

Tilapia is the common name broadly applied to a group of cichlid fishes native to Africa, the Mediterranean, and the Middle East. Tilapia are some of the oldest cultured fishes, as depicted in line drawings found in Egyptian tombs that date back to 2000 BC. This group consists of three economically important genera, which are taxonomically distinguished according to their reproductive behaviors: Tilapia, Oreochromis, and Sarotherodon, all are commonly known as “tilapia.” Tilapia are biparental caring-substrate spawners; Oreochromis are generally maternal mouth brooders; and Sarotherodon are generally paternal or biparental mouth brooders. Currently, Nile tilapia (Oreochromis niloticus; fig. 1) and …

Tilapia: Environmental Biology And Nutritional Requirements, Kamal Mjoun, Kurt A. Rosentrater, Michael L. Brown

Kurt A. Rosentrater

Tilapia is one of the most widely cultured fish in the world. Currently, farmed tilapia represents more than 75% of world tilapia production (FAO, 2009), and this contribution has been exponentially growing in recent years. Several factors have contributed to the rapid global growth of tilapia. Tilapia are easily cultured and highly adaptable to a wide range of environmental conditions. Tilapia feed on a wide variety of dietary sources, including phytoplankton, periphyton, zooplanktons, larval fish, and detritus. Adult tilapia are principally herbivorous but readily adapt to complete commercial diets based on plant and animal protein sources. In the United States, …

Twin-Screw Extrusion Processing Of Distillers Dried Grains With Solubles (Ddgs)-Based Yellow Perch (Perca Flavescens) Feeds, Ferouz Y. Ayadi, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, Michael L. Brown

Kurt A. Rosentrater

Due to tremendous increases in global aquaculture production, compounded with limited availabilities of fish meal for fish feed, the need for alternative protein sources cannot be disregarded. Toward that end, twin-screw extrusion studies were performed to investigate the production of nutritionally-balanced feeds for juvenile yellow perch (Perca flavescens). Five isocaloric (~ 3.06 kcal/g) ingredient blends, adjusted to a similar protein content of 36.7% db, were formulated with 0, 10, 20, 30, and 40% distillers dried grains with solubles (DDGS) at an initial moisture content of 5-7% db, with appropriate amounts of fish meal, fish oil, whole wheat flour, corn gluten …

Twin-Screw Extrusion Processing Of Distillers Dried Grains With Solubles (Ddgs)-Based Rainbow Trout (Oncorhynchus Mykiss) Feeds, Ferouz Y. Ayadi, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater, Michael L. Brown

Kurt A. Rosentrater

The world’s increasing demand for seafood products has resulted in rising demands for fish meal for fish feed, which must be compensated for by searching for effective alternative protein sources. In this study, twin-screw extrusion trials were conducted to study the production of nutritionally-balanced feeds for rainbow trout fingerlings (Oncorhynchus mykiss). Six isocaloric (~ 4.61 kcal/g) ingredient blends with a similar protein content of 52.8% db were formulated with 0, 10, 20, 30, 40 and 50% distillers dried grains with solubles (DDGS) and other ingredients (i.e. fish meal, fish oil, whole wheat flour, corn gluten meal, and vitamin and mineral …

Extrusion Of Aquafeeds Containing Corn Distillers Dried Grains With Solubles (Ddgs) For Nile Tilapia, Kurt A. Rosentrater, Mehmet C. Tulbek

Kurt A. Rosentrater

As the global population grows, there is an increasing need to produce food; aquaculture can help meet some of this growing demand. The majority of protein for aquafeeds is fish meal. But due to growing costs and potential scarcity of fish meal supplies in coming years, there is an impending need to find alternative protein sources. DDGS, a coproduct of the fuel ethanol industry, may be suitable for this purpose. The objective of this study was to examine the effect of extrusion processing conditions on two feed blends containing DDGS and other ingredients. Based on previous research, two optimized aquafeeds …

Effects Of Processing Conditions On Single Screw Extrusion Of Feed Ingredients Containing Ddgs, Nehru Chevanan, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan

Kurt A. Rosentrater

Distillers dried grains with solubles (DDGS), a feed coproduct from the fuel ethanol industry, has been shown to be a viable potential alternative protein source for aquaculture feeds. To investigate this, three isocaloric (3.5kcal/g) ingredient blends containing 20, 30, and 40% DDGS, with a net protein adjusted to 28% (wet basis, wb), were prepared for use as Nile tilapia feed. Extrusion processing was then conducted using three DDGS contents (20, 30, and 40%, wb), three moisture contents (15, 20, and 25%, wb), three barrel temperature gradients (90–100–100°C, 90–130–130°C, and 90–160–160°C), and five screw speeds (80, 100, 120, 140, and 160rpm) …

Twin Screw Extrusion Of Ddgs-Based Aquaculture Feeds, S. Kannadhason, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, Michael L. Brown

Kurt A. Rosentrater

Six isocaloric (3.65 kcal/g), isonitrogenous (35% dry-basis [db] protein), ingredient blends were prepared with 0, 17.5, 20, 22.5, 25, and 27.5% distiller’s dried grains with solubles (DDGS) and other ingredients (soybean meal, corn, fish meal, whey, soybean oil, vitamin and mineral mix). The blends were moisture balanced to 15% db, then extruded in a twin screw extruder using a 2 mm die at 190 rpm, and a 3 mm die at 348 rpm. Analyses of the extrudates included moisture content, expansion ratio, unit density, bulk density, sinking velocity, color (L*, a*, and b*), water absorption, water solubility, and pellet durability …

The Effects Of Extrusion Processing Of Distillers Dried Grains With Solubles (Ddgs)-Based Yellow Perch (Perca Flavescens) Feeds, Ferouz Y. Ayadi, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater, Michael L. Brown

Kurt A. Rosentrater

This study was conducted to investigate the production of balanced diets for juvenile yellow perch (Perca flavescens) feeds. Six isocaloric (~ 3.21 kcal/g), isonitrogenous (31.5% db) ingredient blends were formulated with 0, 10, 20, 30, 40, and 50% distillers dried grains with solubles (DDGS) at a feed moisture content of 60-65% db, with appropriate amounts of soybean meal, fish meal, vitamin and mineral mix. Extrusion cooking was performed using a laboratory-scale single screw extruder at a constant barrel temperature profile of 40ºC-90ºC-100ºC, and a constant screw speed of 230 rpm (24.1 rad/s). During processing the mass flow rate was determined, …

Extrusion Studies Of Aquaculture Feed Using Distillers Dried Grains With Solubles And Whey, Nehru Chevanan, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater

Kurt A. Rosentrater

Three isocaloric (3.5 kcal/g) ingredient blends containing 20, 30, and 40% distiller-dried grains with solubles (DDGS) along with 5% whey were prepared with a net protein content adjusted to 28% (wet basis [wb]). Other ingredients in the blends included soy flour, corn flour, fish meal, vitamin, and mineral mix. These blends were extruded in a single-screw extruder at 15, 20, and 25% (wb) moisture content and at 130 and 160 rpm screw speeds. Compared to previous research, the durability and unit density of the extrudates in this study were found to increase substantially by the addition of whey to the …

Filthy Cellars And Healthy Pets: Relationships Between Public Health, Pets And Veterinarians In Cincinnati Prior To World War I, Kelly Wenig

Kelly Wenig

In 1868, Board of Health officials in the city of Cincinnati declared that all animals had to be removed from the basements of city residences. In his report on the state of the city, health officer William C. Clendenin commented that “[t]he extent to which cellars are used throughout the city as depositories for rubbish and filth is truly surprising; --many respectable people [keep] geese, chickens, dogs, and even calves in their cellars…Filthy cellars, especially when they are very damp, are a very certain cause of sickness.”1 For most of the nineteenth century, cows, chickens, sheep, dogs and cats were …

Effects Of Ingredients And Extrusion Parameters On Properties Of Aquafeeds Containing Ddgs And Corn Starch, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, S. Kannadhason

Kurt A. Rosentrater

Isocaloric (3.05 kcal g-1) ingredient blends were factorially formulated using 3 levels each of DDGS (20, 25 and 30% db), protein (30, 32.5 and 35% db) and feed moisture content (25, 35 and 45% db), along with appropriate quantities of corn starch, soybean meal, fish meal, whey, vitamin and mineral mix to produce a balanced diet for tilapia feed. The ingredient blends were extruded using a laboratory-scale single screw extruder with varying screw speeds (100, 150 and 200 rpm) and extruder barrel temperatures (100, 125 and 150°C). The resulting extrudates were subjected to extensive analyses of physical properties, which included …

Performance Characteristics Of Nile Tilapia (Oreochromis Niloticus) Fed Diets Containing Graded Levels Of Fuel-Based Distillers Dried Grains With Solubles, Travis W. Schaeffer, Michael L. Brown, Kurt A. Rosentrater

Kurt A. Rosentrater

A feeding trial was performed to investigate levels of corn-based Distillers Dried Grains with solubles (DDGS), a co-product of fuel ethanol manufacturing, used as a nutrient source for juvenile Nile tilapia (Oreochromis niloticus). Three isocaloric (15.7±0.7 MJ kg-1 dry matter), isonitrogenous (29.6±1.0% crude protein) experimental diets were formulated to contain 20, 30 and 40% DDGS; a commercial diet containing fishmeal was used as a reference diet. Glass aquaria (110 L) were stocked with Nile tilapia (mean weight = 6.7 g) with four replicates per diet. Weight gains, conversion ratios and hepatosomatic indices did not significantly differ between fish fed 20% …

Effects Of Ingredients And Extrusion Parameters On Aquafeeds Containing Ddgs And Tapioca Starch, S. Kannadhason, Kasiviswanathan Muthukumarappan, Kurt A. Rosentrater

Kurt A. Rosentrater

Isocaloric (3.05 kcal g-1) ingredient blends were factorially formulated using three levels each of DDGS (20, 25 and 30% db), protein (30, 32.5 and 35% db) and feed moisture content (25, 35 and 45% db), along with appropriate quantities of tapioca starch, soybean meal, fish meal, whey, vitamin and mineral mix to produce a balanced diet for tilapia feed. The ingredient blends were extruded using a laboratory-scale single screw extruder with varying screw speeds (100, 150 and 200 rpm) and extruder barrel temperatures (100, 125 and 150°C). The resulting extrudates were subjected to extensive analyses of physical properties, which included …

Effects Of Ingredients And Extrusion Parameters On Aquafeeds Containing Ddgs And Potato Starch, Kurt A. Rosentrater, Kasiviswanathan Muthukumarappan, S. Kannadhason

Kurt A. Rosentrater

Isocaloric (3.05 kcal g-1) ingredient blends were factorially formulated using three levels each of DDGS (20, 25 and 30% db), protein (30, 32.5 and 35% db) and feed moisture content (25, 35 and 45% db), along with appropriate quantities of potato starch, soybean meal, fish meal, whey, vitamin and mineral mix to produce a balanced diet for tilapia feed. The ingredient blends were extruded using a laboratory-scale single screw extruder with varying screw speeds (100, 150 and 200 rpm) and extruder barrel temperatures (100, 125 and 150°C). The resulting extrudates were subjected to extensive analyses of physical properties, which included …