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Full-Text Articles in Life Sciences

Hundreds Of Scn-Resistant Soybean Varieties Available For 2014, Gregory L. Tylka Oct 2014

Hundreds Of Scn-Resistant Soybean Varieties Available For 2014, Gregory L. Tylka

Integrated Crop Management News

The soybean cyst nematode (SCN) is a serious yield-reducing pathogen of soybeans. It is present in many fields throughout the Midwest, wherever soybeans are grown.

To produce profitable soybean yields in fields infested with SCN, farmers should grow SCN-resistant soybean varieties. SCN-resistant soybeans can produce high yields while keeping SCN population densities from increasing.


Dahp Oxime: A Transition State Mimic Inhibitor Of Dahp Synthase, Naresh Balachandran Oct 2014

Dahp Oxime: A Transition State Mimic Inhibitor Of Dahp Synthase, Naresh Balachandran

Open Access Dissertations and Theses

The rise of bacterial infections and increase of antibiotic resistant bacteria has become a major problem in the treatment of bacterial infections. The use and overuse of antibiotics, and the inherent ability of bacteria to adapt to their environment, have lead to the emergence of strains that are resistant to all antibiotics. Ideally, new targets for antibacterial drug therapy would be essential to the virulence of most or all bacteria. That is, antibiotics exploiting these targets would have broad spectrum activity. 3-Deoxy-D-arabinoheptulosonate-7- phosphate (DAHP) synthase could be such a target. This enzyme catalyzes the condensation of erythrose 4-phosphate (E4P) and ...


A Characterization Of The Dynamic Interaction Between The Pro-Apoptotic Protein Bid And The Mitochondrial Outer Membrane, Aisha Shamas-Din Ms. Oct 2014

A Characterization Of The Dynamic Interaction Between The Pro-Apoptotic Protein Bid And The Mitochondrial Outer Membrane, Aisha Shamas-Din Ms.

Open Access Dissertations and Theses

Bcl-2 family of proteins regulate apoptosis at the level of the mitochondrial outer membrane (MOM) through both protein-protein and protein-membrane interactions. While the role of the membrane as the “locus of action” has been recognized, the detailed molecular mechanisms and the consequences of the interactions of Bcl-2 family members with the membrane are yet to be fully understood. The findings presented here focus on the dynamic interactions of Bcl-2 proteins, most notably tBid with the MOM, and their functional significance on mitochondrial outer membrane permeabilization. We show that the activation of tBid is a multi-step process that is regulated by ...


Investigation Of Competence Heterogeneity In Streptococcus Milleri Group Clinical Isolates, Anne-Marie G. Lacroix Oct 2014

Investigation Of Competence Heterogeneity In Streptococcus Milleri Group Clinical Isolates, Anne-Marie G. Lacroix

Open Access Dissertations and Theses

The Streptococcus Milleri/Anginosus group (SMG) includes Streptococcus anginosus, Streptococcus constellatus and Streptococcus intermedius. The SMG is found in healthy individuals but these bacteria are most known clinically for being associated with invasive disease and more recently, airway infections including cystic fibrosis (CF). The SMG like many other streptococci are naturally competent, being able to actively bind, uptake and integrate extracellular DNA. Competence regulation involves a competence-stimulating peptide (CSP) derived from the ComC precursor and a two- component signaling system (a histidine kinase ComD and its response regulator ComE). In this study, I examined the distribution of CSP/ComD sequences ...


The Impact Of Agriculture On African Civilization In The 21st Century, Andrew S. Targowski Aug 2014

The Impact Of Agriculture On African Civilization In The 21st Century, Andrew S. Targowski

International Conference on African Development

The purpose of this investigation is to define the impact of agriculture on African Civilization in the 21st century. The methodology is based on an interdisciplinary big-picture view of the African Civilizations developments and interdependency. Among the findings are: Sub-Saharan Africa’s only every second individual has an access to clean and enough water and electricity therefore agriculture is not productive and cannot reduce poverty and provide living sustainable environment. Practical implication: To develop agriculture as an economic engine for 65 plus nationally employed in this activity, first one must develop Integrated Infrastructure of Agriculture (IIA) at the level ...


Panicum Mosaic Virus Complex And Biofuels Switchgrass, Catherine Louise Stewart Aug 2014

Panicum Mosaic Virus Complex And Biofuels Switchgrass, Catherine Louise Stewart

Biological Systems Engineering--Dissertations, Theses, and Student Research

New switchgrass (Panicum virgatum L.) cultivars are being developed for use as a biofuel pyrolysis feedstock. Viral pathogens have been reported in switchgrass, but their importance in biofuel cultivars is not well known. In 2012 surveys of five switchgrass breeding nurseries in Nebraska, plants with mottling and stunting— symptoms associated with virus infection—had an incidence of symptomatic plants within fields as high as 59%. Leaves from 120 symptomatic plants were analyzed by ELISA for Panicum mosaic virus (PMV) and four other viruses known to infect switchgrass. Most samples (87%) were positive for PMV, and fewer than 8% for the ...


Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates Aug 2014

Rna Interference As A Tool For The Functional Analysis Of Genes In The Colorado Potato Beetle, Leptinotarsa Decemlineata (Say), Ashley Danielle Yates

Dissertations and Student Research in Entomology

RNA interference (RNAi) is a naturally occurring phenomenon in eukaryotes in which a double-stranded RNA (dsRNA) suppresses the expression of a target gene. RNAi has markedly changed the way in which functional genetics studies are performed, especially in non-model organisms. In insects, the efficacy of RNAi is influenced by several factors, including the species and the methods of dsRNAs delivery.

The Colorado potato beetle (CPB), Leptinotarsa decemlineata (Say), is a pest of the plant family Solanaceae. RNAi in the CPB is of interest for potential use in insect management and as a tool to study the interaction with host plants ...


Characterization Of Protein-Protein Interactions Mediated By The Second Exon Of The Varicella-Zoster Virus Orf45/42 Terminase Subunit, Elizabeth A. Syster-Clevenger Aug 2014

Characterization Of Protein-Protein Interactions Mediated By The Second Exon Of The Varicella-Zoster Virus Orf45/42 Terminase Subunit, Elizabeth A. Syster-Clevenger

Master's Theses

Varicella-zoster virus (VZV), a member of the Herpesviridae, is ubiquitous human pathogen with significant morbidity and mortality in healthy and immunocompromised individuals. Primary infection causes a systemic viremia and skin rash known as varicella, and more commonly referred to as chickenpox. Latency is established in the nervous system during primary infection and can reactivate to cause zoster, more commonly known as shingles. Current treatments for VZV infection target viral DNA replication, primarily the DNA polymerase. However, novel small molecule inhibitors that target other viral replication processes, for example viral DNA encapsidation, offer additional therapeutic options. Seven conserved open reading frames ...


Energy Content Of Reduced-Fat Dried Distillers Grains And Solubles For Lactating Dairy Cows And Effects On Energy And Nitrogen Balance, Alison J. Foth Aug 2014

Energy Content Of Reduced-Fat Dried Distillers Grains And Solubles For Lactating Dairy Cows And Effects On Energy And Nitrogen Balance, Alison J. Foth

Theses and Dissertations in Animal Science

Eight Holstein and 8 Jersey multiparous, lactating cows were used to complete 56 energy balances to determine the energy content of reduced-fat distillers grains and solubles (RFDDGS). A repeated switchback design was used to compare treatments with and without RFDDGS. Diets consisted of 24.2 % corn silage, 18.4 % alfalfa hay, 6.94 % brome hay with either 22.9 % rolled corn and 14.8 % soybean meal (Control), or 8.95 % rolled corn, 28.8 % RFDDGS, and 0 % soybean meal (Co-P; DM basis). The inclusion of RFDDGS did not affect (P = 0.86) DMI averaging 21.4 ± 0.53 kg DM ...


Effects Of Sorting Prior To Feeding Zilpaterol Hydrochloride And Effects Of Different Implant Strategies On Feedlot Performance And Carcass Characteristics Of Steers And Heifers, Fred H. Hilscher Jr Aug 2014

Effects Of Sorting Prior To Feeding Zilpaterol Hydrochloride And Effects Of Different Implant Strategies On Feedlot Performance And Carcass Characteristics Of Steers And Heifers, Fred H. Hilscher Jr

Theses and Dissertations in Animal Science

Two experiments evaluated the effects of sorting and feeding Zilpaterol hydrochloride (ZH) to steers on feedlot performance and carcass characteristics. In Exp. 1, treatments compared unsorted cattle either fed ZH or not, and three treatments utilizing 1 sort at the beginning, middle or end of the finishing period. In Exp. 2, treatments compared unsorted cattle fed ZH, and two treatments that utilized 1 sort based on initial BW or 4 sorts at the end of the finishing period. Feeding ZH increased BW, HCW and percentage of overweight carcasses; however, sorting decreased carcass weight standard deviations, and percent overweight carcasses. Feeding ...


The Evolution Of Genomic Imprinting: Theories, Predictions And Empirical Tests, M M. Patten, L Ross, J P. Curley, David C. Queller, R Bonduriansky, J B. Wolf Aug 2014

The Evolution Of Genomic Imprinting: Theories, Predictions And Empirical Tests, M M. Patten, L Ross, J P. Curley, David C. Queller, R Bonduriansky, J B. Wolf

Biology Faculty Publications

The epigenetic phenomenon of genomic imprinting has motivated the development of numerous theories for its evolutionary origins and genomic distribution. In this review, we examine the three theories that have best withstood theoretical and empirical scrutiny. These are: Haig and colleagues’ kinship theory; Day and Bonduriansky’s sexual antagonism theory; and Wolf and Hager’s maternal–offspring coadaptation theory. These theories have fundamentally different perspectives on the adaptive significance of imprinting. The kinship theory views imprinting as a mechanism to change gene dosage, with imprinting evolving because of the differential effect that gene dosage has on the fitness of matrilineal ...


Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon Aug 2014

Physical Activity In Individuals With Down Syndrome, Chelsea B. Spurgeon

University of Tennessee Honors Thesis Projects

No abstract provided.


Feeding Condensed Distillers Solubles To Feedlot Finishing Steers And The Effects Of Feed Additives In Adaptation Diets, Marie E. Harris Aug 2014

Feeding Condensed Distillers Solubles To Feedlot Finishing Steers And The Effects Of Feed Additives In Adaptation Diets, Marie E. Harris

Theses and Dissertations in Animal Science

By-products from the dry-milling ethanol process can be used in cattle diets to replace corn. There is a significant interaction between corn processing methods as CCDS concentration increases in the diet. Improvements are observed for SFC in final BW, ADG, and G:F over DRC. An experiment was conducted to determine if greater concentrations of CCDS could be fed in SFC based diets and maintain or improve performance. Performance and carcass characteristics were evaluated with increasing concentrations of CCDS at 0, 9, 18, 27, or 36% in place of SFC in feedlot finishing diets. As CCDS concentration increased, DMI decreased ...


Single Tube, Multiple Enzyme Reaction For Detection Of Uv And Oxidative Damage In Forensic Physiological Stains, Nicholas J. Eurek Aug 2014

Single Tube, Multiple Enzyme Reaction For Detection Of Uv And Oxidative Damage In Forensic Physiological Stains, Nicholas J. Eurek

Theses and Dissertations in Biochemistry

For decades, the use of DNA as a biological tool has revolutionized forensic investigations. The primary use of this genetic evidence is for identification of a victim or suspect through short tandem repeat (STR) profiling. However, the usefulness of this evidence can be compromised through inhibition of PCR, damage to the DNA, or low copy number. Here, we investigate damage induced to DNA by environmental factors. UV light is known to damage DNA by the formation of cyclobutane pyrimidine dimers, 6-4 photoproducts, and strand breaks. These lesions can stall polymerase action or misincorporate bases during extension. Oxidative damage is also ...


Performance Assessment Of Some Flax (Linum Usitatissimum L.) Varieties Using Cluster Analysis Under Sandy Soil Conditions, Omar Maghawri Ibrahim Hassan Dr. Jul 2014

Performance Assessment Of Some Flax (Linum Usitatissimum L.) Varieties Using Cluster Analysis Under Sandy Soil Conditions, Omar Maghawri Ibrahim Hassan Dr.

Dr. Omar Maghawri Ibrahim

To assess the performance and the variability of 49 flax varieties based on agronomic parameters using cluster analysis, a field experiment was conducted in 2011/2012 and 2012/2013 winter seasons at the farm of the National Research Center at Nubria district, Albehira Governorate, Egypt. The results revealed high significant difference among all flax varieties in all the studied characters. Letwania-9 and Evelen cultivars surpassed all other varieties in seed and oil yields/ fed. On the other hand, D variety gave the lowest value of seed yield/fed and F variety gave the lowest values of oil% and oil yield ...


Height And Calories In Early Childhood, Andrew S. Griffen Jul 2014

Height And Calories In Early Childhood, Andrew S. Griffen

Grand Challenges Canada Economic Returns to Mitigating Early Life Risks Project

This paper estimates a height production function using data from a randomized nutrition intervention conducted in rural Guatemala from 1969 - 1977. Using the experimental intervention as an instrument, the IV estimates of the effect of calories on height are an order of magnitude larger than the OLS estimates. Information from a unique measurement error process in the calorie data, counterfactuals results from the estimated model and external evidence from migration studies suggest that the divergence between the OLS and IV estimates is driven by the LATE interpretation of IV. Attenuation bias corrected OLS estimates of the height production function imply ...


The Effect Of Angiotensin Converting Enzyme Genotype On Aerobic Capacity Following High Intensity Interval Training, Nicole Goddard, Matthew Baker, Tim Higgins, Christian Cobbold Jul 2014

The Effect Of Angiotensin Converting Enzyme Genotype On Aerobic Capacity Following High Intensity Interval Training, Nicole Goddard, Matthew Baker, Tim Higgins, Christian Cobbold

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 250-259, 2014. Obesity increases the risk of developing type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD). Physical activity can reduce T2DM and CVD risk, and increase aerobic capacity, a significant predictor of all-cause mortality and morbidity. High intensity interval training (HIIT) produces similar improvements in aerobic capacity to continuous moderate exercise (CME). Different genotypes of angiotensin converting enzyme (ACE) have been implicated in improving aerobic capacity and therefore predicted health. This study investigated the effects of different ACE genotypes on the impact of 6 weeks of HIIT on aerobic capacity, and thus ...


Contributions Of After School Programs To The Development Of Fundamental Movement Skills In Children, E Jean Burrows, Melanie R. Keats, Angela M. Kolen Jul 2014

Contributions Of After School Programs To The Development Of Fundamental Movement Skills In Children, E Jean Burrows, Melanie R. Keats, Angela M. Kolen

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 236-249, 2014. Fundamental movement skill (FMS) proficiency or the ability to perform basic skills (e.g., throwing, catching and jumping) has been linked to participation in lifelong physical activity. FMS proficiency amongst children has declined in the previous 15 years, with more children performing FMS at a low-mastery level. These declines may help explain the insufficient levels of participation in health promoting physical activity seen in today’s youth. The after school time period (e.g., 3 to 6 p.m.), is increasingly considered an opportune time for physical activity interventions. To date ...


The Energy Expenditure Of Recreational Ballroom Dance, D. E. Lankford, T. W. Bennion, J. King, N. Hessing, L Lee, D. P. Heil Jul 2014

The Energy Expenditure Of Recreational Ballroom Dance, D. E. Lankford, T. W. Bennion, J. King, N. Hessing, L Lee, D. P. Heil

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 228-235, 2014. The popularity of recreational ballroom dancing has increased dramatically in recent years. Yet, relatively little information is known regarding the physiological demands of ballroom dancing. The purpose of this study was to determine the energy requirements for recreational ballroom dancing. 24 participants volunteered including 12 women (mean ± SD: 21 ± 3 yrs, 165.8 ± 7.4 cm, 56.8 ± 11.1 kg) and 12 men (23 ± 1 yr, 175.5 ± 8.4 cm, 78.1 ± 15.6 kg). Gas exchange was recorded using a portable metabolic system during a series of five ...


The Influence Of Exercise Environment And Gender On Mood And Exertion, Thomas G. Plante Phd, Abpp, Marily A. Opezzo Phd, L. Aislinn Diaz, Selena Pistoresi, Michael Santos, Jacqueline E. Fahey, Elizabeth Kay, Briana Britton, Suheel Khan Jul 2014

The Influence Of Exercise Environment And Gender On Mood And Exertion, Thomas G. Plante Phd, Abpp, Marily A. Opezzo Phd, L. Aislinn Diaz, Selena Pistoresi, Michael Santos, Jacqueline E. Fahey, Elizabeth Kay, Briana Britton, Suheel Khan

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 220-227, 2014. This study examined the influence of exercise environment and gender on post-exercise mood and exertion. College student participants (55 females, 49 males) were instructed to pedal a stationary bike at a moderate pace for 20 minutes. Participants were randomly assigned to one of three laboratory conditions: (1) exercising in front of a mirror and posters showing ideal fit body types (i.e., celebrity male and female personal trainers), (2) exercising in front of a mirror only, or (3) a control condition in which participants exercised without a mirror or posters. The ...


Acute Post-Activation Potentiation In Ncaa Division Ii Female Athletes, Kaiya Senn Sygulla, Charles J. Fountaine Jul 2014

Acute Post-Activation Potentiation In Ncaa Division Ii Female Athletes, Kaiya Senn Sygulla, Charles J. Fountaine

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 212-219, 2014. Post-activation potentiation (PAP) is a phenomenon in which the power output of a muscle is immediately enhanced after heavy resistance exercise. Whereas the majority of PAP research has examined males, minimal research exists as to how female athletes respond. Therefore, the purpose of this study was to investigate the acute PAP response of back squats on static squat jump (SSJ) performance among NCAA Division II female athletes. Female athletes (n=29) who were current roster members from basketball, softball, and volleyball, performed 3 SSJ prior to 3 repetitions of the back ...


An Acute Bout Of Self-Myofascial Release In The Form Of Foam Rolling Improves Performance Testing, Corey A. Peacock, Darren D. Krein, Tobin A. Silver, Gabriel J. Sanders, Kyle-Patrick A. Von Carlowitz Jul 2014

An Acute Bout Of Self-Myofascial Release In The Form Of Foam Rolling Improves Performance Testing, Corey A. Peacock, Darren D. Krein, Tobin A. Silver, Gabriel J. Sanders, Kyle-Patrick A. Von Carlowitz

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 202-211, 2014. Recent developments in the strength and conditioning field have shown the incorporation of foam rolling self-myofascial release in adjunct with a dynamic warm-up. This is thought to improve overall training performance; however, minimal research exists supporting this theory. Therefore, determining if an acute bout of foam rolling self-myofascial release in addition to a dynamic warm-up could influence performance is of importance. In order to do so, eleven athletically trained male subjects participated in a two condition, counterbalanced, crossover within-subjects study comparing two particular warm-up routines. The two warm-up routines compared were ...


The Physiologic And Behavioral Implications Of Playing Active And Sedentary Video Games In A Seated And Standing Position, Gabriel J. Sanders, Michael Rebold, Corey A. Peacock, Megan L. Williamson, Antonio Santo, Jacob E. Barkley Jul 2014

The Physiologic And Behavioral Implications Of Playing Active And Sedentary Video Games In A Seated And Standing Position, Gabriel J. Sanders, Michael Rebold, Corey A. Peacock, Megan L. Williamson, Antonio Santo, Jacob E. Barkley

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 194-201, 2014. Previous studies have assessed physiologic response while playing video games per manufacturer instructions with participants standing during active video game play and seated during sedentary game play. It is not known whether an assigned seated or standing position affects positional preference and oxygen consumption (VO2) while gaming. The purpose of the study was to assess VO2 and preference of playing active and sedentary video games in a seated and standing position. VO2 was assessed in 25 participants during four, 20-minute conditions; resting, PlayStation 2 Madden NFL Football 2011, Nintendo Wii-Sports Boxing ...


Effect Of Long-Term Physical Exercise On Blood Pressure In African Americans, Taunjah P. Bell, Katharine A. Mcintyre, Rosamary Hadley Jul 2014

Effect Of Long-Term Physical Exercise On Blood Pressure In African Americans, Taunjah P. Bell, Katharine A. Mcintyre, Rosamary Hadley

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 186-193, 2014. Long-term physical exercise has been shown to noticeably reduce blood pressure (BP) and remarkably attenuate symptoms of hypertension. It is believed that physical exercise induces these beneficial effects by increasing the blood supply to the brain, enhancing the release of growth factors from skeletal muscles into the bloodstream, facilitating neurogenesis, stimulating angiogenesis, and influencing endothelial cell proliferation and subsequent endothelial cell membrane permeability. Previous findings also revealed that physical exercise significantly elevates brain-derived neurotrophic factor (BDNF) concentrations which appear to increase dramatically in BP-sensing neurons during hypertension. Elevating BDNF levels is ...


Cardiovascular And Metabolic Demands Of The Kettlebell Swing Using A Tabata Interval Versus A Traditional Resistance Protocol, Howard A. Fortner, Jeanette Salgado, Angelica M. Holmstrup, Michael E. Holmstrup Phd Jul 2014

Cardiovascular And Metabolic Demands Of The Kettlebell Swing Using A Tabata Interval Versus A Traditional Resistance Protocol, Howard A. Fortner, Jeanette Salgado, Angelica M. Holmstrup, Michael E. Holmstrup Phd

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 179-185, 2014. Tabata (TAB) training, consisting of eight cycles of 20 seconds of maximal exercise followed by 10 seconds of rest, is time-efficient, with aerobic and anaerobic benefit. This study investigated the cardiovascular and metabolic demands of a TAB versus traditional (TRAD) resistance protocol with the kettlebell swing. Fourteen young (18-25y), non-obese (BMI 25.7±0.8 kg/m2) participants reported on three occasions. All testing incorporated measurements of HR, oxygen consumption, and blood lactate accumulation. Each participant completed Tabata kettlebell swings (male- 8kg, female- 4.5kg; 8 intervals; 20s maximal repetitions ...


Is There An Optimal Whole-Body Vibration Exposure ‘Dosage’ For Performance Improvement?, R Hugh Morton, Darryl J. Cochrane Jul 2014

Is There An Optimal Whole-Body Vibration Exposure ‘Dosage’ For Performance Improvement?, R Hugh Morton, Darryl J. Cochrane

International Journal of Exercise Science

International Journal of Exercise Science 7(3) : 169-178, 2014. Whole-body vibration exposure has been shown to improve performance in vertical jumping and knee extensions. Some studies have addressed the question of dose optimality, but are inconclusive and inappropriately designed. Our purpose was to more thoroughly seek an optimum combination of duration, amplitude and frequency of exposure to side-alternating whole-body vibration. We used experimental designs constructed for response surface fitting and optimisation, using both blocked and unblocked second order central composite designs with 12 participants. Immediately after each exposure a discomfort index was recorded, then peak and average torque, peak and ...


Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 1 Of 2, George Mcnamara Jul 2014

Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 1 Of 2, George Mcnamara

George McNamara

Timelapse data - GM imaging cytometer needs stitching part 1 of 2

Imaging challenge: align multiple time series over time to overcome "stage slop" in the imaging cytometer used in this experiment.

http://works.bepress.com/gmcnamara/53 (this)

and

http://works.bepress.com/gmcnamara/54 (next)

are zip files containing 8-bit stacks (MetaMorph multiplane TIFF files) acquired on an imaging cytometer.

"53" is the brightfield data, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6).

"54" is GFP fluorescence, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). The GFP fluorescence decreases over time ...


Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara Jul 2014

Timelapse Data - Gm Imaging Cytometer Needs Stitching Part 2 Of 2, George Mcnamara

George McNamara

Timelapse data - GM imaging cytometer needs stitching part 2 of 2

Imaging challenge: align multiple time series over time to overcome "stage slop" in the imaging cytometer used in this experiment.

http://works.bepress.com/gmcnamara/53 (previous) and http://works.bepress.com/gmcnamara/54 (this)

are zip files containing 8-bit stacks (MetaMorph multiplane TIFF files) acquired on an imaging cytometer.

"53" is the brightfield data, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). "54" is GFP fluorescence, raw and automatic aligned stacks in MetaMorph (Apps menu, MM7.8.6). The GFP fluorescence decreases over time ...


The Prediction Of B-Cell Epitope Via Biostatistical And Bioinformatic Methodology And Applications, Bo Yao Jul 2014

The Prediction Of B-Cell Epitope Via Biostatistical And Bioinformatic Methodology And Applications, Bo Yao

Dissertations and Theses in Biological Sciences

By creating antibodies against antigens, B-cells, also named B-lymphocytes, play an important role in the immune system to fight against foreign invasion to the host body. Within the antigen specific to a certain B-cell antibody, the sections recognized and bound by antibody are called B-cell epitopes. As antigenic determinants, B-cell epitope identification is of vital importance in many immunological processes, such as vaccine design, immunodiagnostic tests, and antibody production. Towards this goal, biologists and immunologists have applied a variety of methods to identify B-cell epitopes through both experiments and bioinformatic predictions.

There are two kinds of B-cell epitopes: linear (continuous ...


Quantitative Ultrasound: Measurement Considerations For The Assessment Of Muscular Dystrophy And Sarcopenia, Michael O. Harris-Love, Reza Monfaredi, Catheeja Ismail, Marc R. Blackman, Kevin Cleary Jul 2014

Quantitative Ultrasound: Measurement Considerations For The Assessment Of Muscular Dystrophy And Sarcopenia, Michael O. Harris-Love, Reza Monfaredi, Catheeja Ismail, Marc R. Blackman, Kevin Cleary

Exercise Science Faculty Publications

Diagnostic musculoskeletal ultrasound has potential clinical utility in characterizing pathological muscle tissue. Sonography has been long proposed as method of assessing muscle damage due to neuromuscular diseases such as muscular dystrophy, and more recently, changes in body and tissue composition associated with muscle wasting disorders such as sarcopenia. The use of quantitative ultrasound as an adjunct diagnostic procedure has different technical challenges than the traditional use of ultrasound in clinical medicine. Operator-dependent technique and variation are critical considerations when obtaining measures of echointensity (i.e., tissue composition estimates) and tissue dimensions (i.e., muscle thickness) – key elements of the ultrasound ...