Life Sciences Commons^™

Ligand-Receptor Interactions For Supramolecular Disassembly With Applications In Screening And Drug Delivery, Diego F. Amado Torres

Diego Amado Torres

Proteins have the capacity to bind specific sets of compounds known as ligands, these are small molecules with a recurrent theme in their molecular design that is a characteristic exploited here to (i) identify particular affinities of small molecules for proteins with the aim of using them as ligands, inhibitors, or targeting moieties in more complex systems by means of a methodology that screens small molecules based on protein affinity; (ii) decorate a self-assembling supramolecular system at different positions, making it responsive to a complementary protein with the aim of exploring differences in disassembly and sensitivity of the release of ...

Initial Characterization Of A Conserved Active Site Residue For The Cdc34 Ubiquitin Conjugating Enzyme, Arvin Akoopie

Theses

Ubiquitin-conjugating enzymes (E2s) covalently modify protein substrates with ubiquitins. The active site cysteine residues on E2s are essential for catalyzing the transfer of ubiquitin from the E2 active site onto the protein substrate, however there is a limited amount of information available concerning additional active site residues for E2s that may also participate in catalysis. Cdc34 is an essential E2 that has merited the lion’s share of attention for biochemical analysis of the E2 family. Previous phylogenetic analysis of Cdc34 amino acid sequences has identified an invariably conserved histidine residue close to the active site cysteine in the primary ...

Influence Of Aba On Calcium Binding In Tomato Fruit And Its Impact On Fruit Texture, Kendall Cressman

University of Tennessee Honors Thesis Projects

No abstract provided.

Health Monitoring Of Seasoned Sows In A New And Unfamiliar Environment, Rebecca Kocak

University of Tennessee Honors Thesis Projects

No abstract provided.

Evaluation Of Novel Multi-Dimensional Tissue Culturing Methods Using Autonomously Bioluminescent Human Cell Lines, James Dean Webb

University of Tennessee Honors Thesis Projects

No abstract provided.

Boundaries Of A Complex World, Andrei Ludu

Andrei Ludu

No abstract provided.

The Biology Of Dendritic Cell Subsets In Allergen-Induced Asthma, Benny Dua

Open Access Dissertations and Theses

Asthma is an inflammatory disorder of the airways, and there has been growing insight into the cellular and molecular mechanisms underlying the inflammatory basis of this disease. Research into the inflammatory mechanisms of asthma has progressively shifted focus from downstream effectors, such as mast cells and eosinophils, up to Th2 lymphocytes and their proallergic cytokines. Even more upstream in the allergic cascade are dendritic cells (DCs), potent APCs that orchestrate immune responses. Evidence supporting a role of DCs in regulating airway allergic inflammation is derived mainly from animal studies. In animal models of asthma, myeloid DCs (mDCs) induce and maintain ...

Determinants Of The Magnitude Of Training Mediated Muscle Hypertrophy, Cameron Mitchell

Open Access Dissertations and Theses

Chronic resistance training leads to muscle hypotrophy in a wide range of populations however most resistance training studies are relatively small in sample size

Three studies were conducted to better understand the sources of this variability. The first study employed a unilateral resistance training model to test the effects of relative training load and volume on the magnitude of hypertrophy and strength gains. This study showed that high relative training loads were no better than low training loads at inducing muscle hypertrophy provided that each set was performed to the point of muscular exhaustion. In agreement with previous finding, strength ...

The Roles Of Hedgehog And Ap-2 Signaling In The Regulation Of Lens Development, Christine L. Kerr

Open Access Dissertations and Theses

Lens development is an intricate process governed by growth factor signaling and a hierarchy of transcription factors that regulate important processes required for normal lens development.

Midline hedgehog (Hh) signaling has been implicated in lens defects including cyclopia and lens degeneration in rodents and fish. A lens specific model of hedgehog signaling has not been examined, and it was unknown whether the lens is able to respond to Hh signals. To investigate this question, and to determine any consequences of abnormal Hh signaling on lens development, a mouse model of constitutively active smoothened in the surface ectoderm and derivatives, (including ...

Nickel Bioaccumulation As A Predictor Of Toxicity, Erin M. Leonard

Open Access Dissertations and Theses

Recently, the focus of metal toxicity has shifted from concentrations within the aqueous environment to bioaccumulation within the organism. In this regard, the Biotic Ligand Model (BLM) relates the binding of metal at specific toxic sites (“ligands”) to eventual toxicity, whereas the Tissue Residue Approach (TRA) relates metal burdens at whole body, tissue, or subcellular levels to eventual toxicity. However, much less is currently known regarding Ni in comparison to other metals. This thesis addresses this knowledge gap by evaluating the use of Ni bioaccumulation as a predictor of toxicity in a number of fish and invertebrate species; bioaccumulation endpoints ...

Nutritional And Contractile Regulation Of Human Muscle Protein Synthesis: Role Of Leucine And Citrulline, Tyler A. Churchward-Venne

Open Access Dissertations and Theses

Amino acids are key nutritional stimuli that are both substrate for muscle protein synthesis (MPS), and signaling molecules that regulate the translational machinery. There is a dose-dependent relationship between protein intake and MPS that differs between young and elderly subjects. The current thesis contains results from three separate studies that were conducted to examine to potential to enhance smaller doses of protein, known to be suboptimal in their capacity to stimulate MPS, through supplementation with specific amino acids, namely leucine and citrulline.

The first two studies examined the potential to enhance the muscle protein synthetic capacity of a smaller, suboptimal ...

Cyb5d2 Possesses Tumour Suppressing Activities, Yen Ting Shen

Open Access Dissertations and Theses

Loss of chromosome 17p is frequently observed in various cancers. One of the most commonly mutated targets p53 is on chromosome 17p13.1. However, studies have also reported loss of the 17p13.2 region in breast and medulloblastoma, thereby suggesting the residence of potential tumour suppressors in 17p13.2. Cytochrome b5 domain containing 2 (CYB5D2) is located on 17p13.2 implying CYB5D2 being a candidate tumour suppressor. CYB5D2 (neuferricin) belongs to the family of membrane associated progesterone receptors (MAPR). The archetypal member of the family, progesterone receptor membrane component 1 (PGRMC1), has been shown to play a role in domains ...

Mental Imagery For The Detection Of Awareness: Evaluating The Convergence Of Functional Magnetic Resonance Imaging And Electroencephalographic Assessments, Amabilis H. Harrison

Open Access Dissertations and Theses

The accurate diagnosis of disorders of consciousness presents substantial difficulty because of the reliance on behaviour-based assessment tools. A patient may be covertly aware but unable to indicate their state due to physical impairments. Neuroimaging researchers have begun to seek alternate methods of assessment that rely on brain responses rather than behavioural ones. To this end, mental imagery has been employed as a voluntary cognitive activity that can be measured with fMRI or EEG to indicate awareness. In this dissertation I examine the advantages and limitations of these two imaging techniques and argue that EEG is more suitable for this ...

In-Package Atmospheric Pressure Cold Plasma Treatment Of Strawberries, N. N. Misra, Paula Bourke, Patrick J. Cullen, Sonal Patil

Articles

The ability to generate low temperature plasma at atmospheric pressure offers new opportunities to decontaminate biological materials, including fresh foods. In this study, strawberries were treated with atmospheric cold plasma (ACP), generated with a 60 kV dielectric barrier discharge (DBD) pulsed at 50 Hz, across a 40 mm electrode gap, generated inside a sealed package containing ambient air (42% relative humidity). The current-voltage characteristics revealed that the plasma operated in the filamentary regime. The background microflora (aerobic mesophillic bacteria, yeast and mould) of strawberries treated for 5 min was reduced by 2 log 10 within 24 h of post-ACP treatment ...

Meta-Analysis Reveals Evolution In Invasive Plant Species But Little Support For Evolution Of Increased Competitive Ability (Eica), E Felker-Quinn, J A. Schweitzer, J K. Bailey

Ecology and Evolutionary Biology Publications and Other Works

Ecological explanations for the success and persistence of invasive species vastly outnumber evolutionary hypotheses, yet evolution is a fundamental process in the success of any species. The Evolution of Increased Competitive Ability (EICA) hypothesis (Blossey and Nötzold 1995) proposes that evolutionary change in response to release from coevolved herbivores is responsible for the success of many invasive plant species. Studies that evaluate this hypothesis have used different approaches to test whether invasive populations allocate fewer resources to defense and more to growth and competitive ability than do source populations, with mixed results. We conducted a meta-analysis of experimental tests of ...

Utilization Of The Invasive Alga Gracilaria Vermiculophylla (Ohmi) Papenfuss By The Native Mud Snail Ilyanassa Obsoleta (Say), Michele Guidone, Christine Newton, Carol S. Thornber

Biology Faculty Publications

The recent invasions of the red alga, Gracilaria vermiculophylla, to the Atlantic and Eastern Pacific Oceans have the potential to significantly alter intertidal and subtidal soft sediment communities. In particular, G. vermiculophylla increases habitat complexity and provides a novel hard substrate in an otherwise two dimensional habitat. Following our observations that the native omnivorous mud snail Ilyanassa obsoleta utilizes G. vermiculophylla for egg capsule deposition, our field surveys demonstrated that the in situ abundance of egg capsules on G. vermiculophylla matched abundances on a native alga Ceramium virgatumandwere at least 11–50 times greater than on all other co-occurring macrophytes ...

Massively Parallel Nonparametric Regression, With An Application To Developmental Brain Mapping, Philip T. Reiss, Lei Huang, Yin-Hsiu Chen, Lan Huo, Thaddeus Tarpey, Maarten Mennes

Philip T. Reiss

We propose a penalized spline approach to performing large numbers of parallel nonparametric analyses of either of two types: restricted likelihood ratio tests of a parametric regression model versus a general smooth alternative, and nonparametric regression. Compared with naively performing each analysis in turn, our techniques reduce computation time dramatically. Viewing the large collection of scatterplot smooths produced by our methods as functional data, we develop a clustering approach to summarize and visualize these results. Our approach is applicable to ultra-high-dimensional data, particularly data acquired by neuroimaging; we illustrate it with an analysis of developmental trajectories of functional connectivity at ...

From The Inside Out, And Through., Dominique Ovalle

The STEAM Journal

These photographs describe “Science” born of consumerism, hijacked by me, economically disenfranchised, or rather—temporarily embarrassed, artist. I was putzing around Malibu—my old college stomping ground, looking for free food; maybe a sample of some gourmet $5 chocolate, and all I got were these photographs.

Stem Art Learning Outcomes, Emily Gottlieb

The STEAM Journal

No abstract provided.

Selected Predictors Of Ankle And Hip Mobilty In Baseball Pitching Performance And Ball Velocity, Derek E. Howell

International Journal of Exercise Science: Conference Proceedings

TACSM Abstract

Selected Predictors of Hip/Ankle Mobility for Baseball Pitching Performance and Ball Velocity

D.E. HOWELL and J.D. MILLER

Human Performance Lab, Department of Exercise of Sport Science, Oklahoma City University, Oklahoma City, OK

Category: Undergraduate

ABSTRACT

Hip and ankle mobility are important variables in ball velocity and pitching performance. Possible indices in hip mobility are passivehip internal rotation (IR) and external rotation (ER). A contributor of ankle mobility is passive ankle dorsi-flexion (PAD). This study examines the relationship of selected indices in hip and ankle mobility for collegiate baseball pitchers with the athlete’s respective coaches ...

Acute Vascular Effects Of Aquatic And Land Treadmill Exercise, Dustin Joubert, Peter Grandjean, Stephen Crouse

International Journal of Exercise Science: Conference Proceedings

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A Comparison Of Heart Rate Slope Between High Fit And Recreational Fit Cyclists, Carpenter Wyatt, Megan Moats, Ashaunta Williams, Frank Wyatt

International Journal of Exercise Science: Conference Proceedings

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A Comparison Of Heart Rate Variability Between High Fit And Recreational Fit Cyclists, Megan Moats, Ashaunta Williams, Carpenter Wyatt, Frank Wyatt

International Journal of Exercise Science: Conference Proceedings

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A Comparison Of Heart Rate Threshold Points Between High Fit And Recreational Fit Cyclists, Ashaunta Williams, Carpenter Wyatt, Megan Moats, Frank Wyatt

International Journal of Exercise Science: Conference Proceedings

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Exercise Attenuated Plasma Oxidized Low-Density Lipoprotein And Myeloperoxidase, Jin K. Park, Yunsuk Koh

International Journal of Exercise Science: Conference Proceedings

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Independent And Combined Effects Of Menhaden Oil And High Fructose On Hepatic Lipid Metabolism, Tayler Nick, Kelsey B. Stout, Robert L. Peters, Justin J. Stout, Joshua S. Wooten

International Journal of Exercise Science: Conference Proceedings

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The Lipogenic Effect Of High-Fructose Consumption On Nafld During Weight Loss, Kelsey B. Stout, Tayler N. Nick, Robert L. Peters, Justin J. Stout, Joshua S. Wooten

International Journal of Exercise Science: Conference Proceedings

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Effect Of Carbohydrate Mouth Rinsing On Muscular Endurance Performance, Cynthia Alanis, Dr. John Smith

International Journal of Exercise Science: Conference Proceedings

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Relationships Between Anthropometric And Performance Variables In Youth: Predictors Of Lower-Body Vertical Jump Peak Power, Alexander S. Drusch, Elias M. Carrillo, Jacob Adam Mota, Kendra D. Olinghouse, Matt S. Stock, Marc Lochbaum, Brennan J. Thompson

International Journal of Exercise Science: Conference Proceedings

ABSTRACT

Anthropometric and physical performance measurements are commonly used for identifying specific physical traits in youth. Laboratory-based tests (e.g., linear velocity transducers), while accurate, provide practical limitations due to high costs and technical necessities. Thus, commonly used field tests may be useful alternatives for assessing fitness/performance characteristics of youth. The purpose of this study was to examine the relationships between anthropometric measures and lower-and upper-body power and strength measures; and to assess the predictive ability of these measures for lower-body vertical jump peak power (PP) output in youth. Thirty-nine pre-adolescent (mean±SD, range: age=7.8±1.7 ...

Energy Expenditure Following Acute Cold Exposure, Steven P. Stelly, Danielle Bravo, Natalie Hines, Loren Koehler, Michael Levi, Donovan L. Fogt

International Journal of Exercise Science: Conference Proceedings

TACSM Abstract -

Energy Expenditure following Acute Cold Exposure

Stelly S, Bravo D, Hines N, Koehler L, Levi M and Fogt D

Exercise Biochemistry & Metabolism Laboratory; Department of Kinesiology, Health & Nutrition; The University of Texas at San Antonio; San Antonio, TX

Category: Masters

Advisor / Mentor: Fogt, Donovan (donovan.fogt@utsa.edu)

ABSTRACT

Whole body energy expenditure (i.e., RMR) increases during acute cold exposure. Whether this increase in energy expenditure persists in the post-cold term in humans due to non-shivering thermogenesis has not yet been evaluated. Therefore we tested the hypothesis that RMR would be different directly following acute cold exposure. RMR (kcal•min ...