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Operations Research, Systems Engineering and Industrial Engineering Commons

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Series

2011

Catalytic bioscavenger

Articles 1 - 2 of 2

Full-Text Articles in Operations Research, Systems Engineering and Industrial Engineering

Protective Efficacy Of Catalytic Bioscavenger, Paraoxonase 1 Against Sarin And Soman Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Lionel Biggemann, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar Jan 2011

Protective Efficacy Of Catalytic Bioscavenger, Paraoxonase 1 Against Sarin And Soman Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Lionel Biggemann, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar

US Army Research

Human paraoxonase 1 (PON1) has been portrayed as a catalytic bioscavenger which can hydrolyze large amounts of chemical warfare nerve agents (CWNAs) and organophosphate (OP) pesticides compared to the stoichiometric bioscavengers such as butyrylcholinesterase. We evaluated the protective efficacy of purified human and rabbit serum PON1 against nerve agents sarin and soman in guinea pigs. Catalytically active PON1 purified from human and rabbit serum was intravenously injected to guinea pigs, which were 30 min later exposed to 1.2 × LCt50 sarin or soman using a microinstillation inhalation exposure technology. Pre-treatment with 5 units of purified human and rabbit serum ...


Recombinant Paraoxonase 1 Protects Against Sarin And Soman Toxicity Following Microinstillation Inhalation Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar Jan 2011

Recombinant Paraoxonase 1 Protects Against Sarin And Soman Toxicity Following Microinstillation Inhalation Exposure In Guinea Pigs, Manojkumar Valiyaveettil, Yonas Alamneh, Peter Rezk, Michael W. Perkins, Alfred M. Sciuto, Bhupendra P. Doctor, Madhusoodana P. Nambiar

US Army Research

To explore the efficacy of paraoxonase 1 (PON1) as a catalytic bioscavenger, we evaluated human recombinant PON1 (rePON1) expressed in Trichoplusia ni larvae against sarin and soman toxicity using microinstillation inhalation exposure in guinea pigs. Animals were pretreated intravenously with catalytically active rePON1, followed by exposure to 1.2 X LCt50 sarin or soman. Administration of 5 units of rePON1 showed mild increase in the blood activity of the enzyme after 30 min, but protected the animals with a significant increase in survival rate along with minimal signs of nerve agent toxicity. Recombinant PON1 pretreated animals exposed to sarin ...