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Operations Research, Systems Engineering and Industrial Engineering Commons

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2008

University of Nebraska - Lincoln

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Articles 31 - 34 of 34

Full-Text Articles in Operations Research, Systems Engineering and Industrial Engineering

Detection Of The Superoxide Radical Anion Using Various Alkanethiol Monolayers And Immobilized Cytochrome C, Xiaojun J. Chen, Alan C. West, Donald M. Cropek, Scott Banta Jan 2008

Detection Of The Superoxide Radical Anion Using Various Alkanethiol Monolayers And Immobilized Cytochrome C, Xiaojun J. Chen, Alan C. West, Donald M. Cropek, Scott Banta

US Army Research

The superoxide radical anion (SO) is a critical biomarker for monitoring cellular stress responses. Electrochemical SO biosensors are frequently constructed through the covalent immobilization of cytochrome c (Cyt c) onto self-assembled monolayers (SAMs); however, a detailed comparison of these systems as well as configuration influence on SO detection is needed to enable robust applications. Two reaction pathways, oxidation of SO by the SAM-modified gold electrode or electron transfer through a protein and monolayer relay, may be involved during the electrochemical detection of SO with Cyt c, depending on the SAM that is used. Although electrodes with SAMs alone can exhibit …


The Phylogeny Of The Four Pan-American Mtdna Haplogroups: Implications For Evolutionary And Disease Studies, Alessandro Achilli, Ugo A. Perego, Claudio M. Bravi, Michael D. Coble, Qing-Peng Kong, Scott R. Woodward, Antonio Salas, Antonio Torroni, Hans-Jürgen Bandelt Jan 2008

The Phylogeny Of The Four Pan-American Mtdna Haplogroups: Implications For Evolutionary And Disease Studies, Alessandro Achilli, Ugo A. Perego, Claudio M. Bravi, Michael D. Coble, Qing-Peng Kong, Scott R. Woodward, Antonio Salas, Antonio Torroni, Hans-Jürgen Bandelt

US Army Research

Only a limited number of complete mitochondrial genome sequences belonging to Native American haplogroups were available until recently, which left America as the continent with the least amount of information about sequence variation of entire mitochondrial DNAs. In this study, a comprehensive overview of all available complete mitochondrial DNA (mtDNA) genomes of the four pan-American haplogroups A2, B2, C1, and D1 is provided by revising the information scattered throughout GenBank and the literature, and adding 14 novel mtDNA sequences. The phylogenies of haplogroups A2, B2, C1, and D1 reveal a large number of sub-haplogroups but suggest that the ancestral Beringian …


Moderate Zinc Restriction Affects Intestinal Health And Immune Function In Lipopolysaccharide-Challenged Mice, Daniel G. Peterson, Angus G. Scrimgeour, James P. Mcclung, Elizabeth A. Koutsos Jan 2008

Moderate Zinc Restriction Affects Intestinal Health And Immune Function In Lipopolysaccharide-Challenged Mice, Daniel G. Peterson, Angus G. Scrimgeour, James P. Mcclung, Elizabeth A. Koutsos

US Army Research

Zinc (Zn) is an essential nutrient that affects immune function, especially within the digestive system, although the underlying mechanisms are not well understood. This study examined the effects of short-term moderate Zn restriction on intestinal health and immune function in lipopolysaccharide (LPS)-challenged mice through plasma cytokine profiling and histological evaluation of intestinal tissue sections. Adult male mice were fed with a Zn-adequate (40 ppm) or a Zn-marginal (4 ppm) diet for 4 weeks, and then a bacterial challenge was simulated by intraperitoneal injection of LPS (10 μg/g body weight [BW]) or saline (control). BW was recorded weekly, and feed intake …


The Influence Of Acidity On Microbial Fuel Cells Containing Shewanella Oneidensis, Justin C. Biffinger, Jeremy Pietron, Orianna Bretschger, Lloyd J. Nadeau, Glenn R. Johnson, Cynthia C. Williams, Kenneth H. Nealson, Bradley R. Ringeisen Jan 2008

The Influence Of Acidity On Microbial Fuel Cells Containing Shewanella Oneidensis, Justin C. Biffinger, Jeremy Pietron, Orianna Bretschger, Lloyd J. Nadeau, Glenn R. Johnson, Cynthia C. Williams, Kenneth H. Nealson, Bradley R. Ringeisen

U.S. Navy Research

Microbial fuel cells (MFCs) traditionally operate at pH values between 6 and 8. However, the effect of pH on the growth and electron transfer abilities of Shewanella oneidensis MR-1 (wild-type) and DSP10 (spontaneous mutant), bacteria commonly used in MFCs, to electrodes has not been examined. Miniature MFCs using bare graphite felt electrodes and nanoporous polycarbonate membranes with MR-1 or DSP10 cultures generated >8W/m3 and ∼400μA between pH 6–7. The DSP10 strain significantly outperformed MR-1 at neutral pH but underperformed at pH 5. Higher concentrations of DSP10 were sustained at pH 7 relative to that of MR-1, whereas at pH …