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Full-Text Articles in Nanoscience and Nanotechnology

Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw Dec 2013

Near-Infrared Surface-Enhanced Fluorescence Using Silver Nanoparticles In Solution, Michael D. Furtaw

Mechanical (and Materials) Engineering -- Dissertations, Theses, and Student Research

Fluorescence spectroscopy is a widely used detection technology in many research and clinical assays. Further improvement to assay sensitivity may enable earlier diagnosis of disease, novel biomarker discovery, and ultimately, improved outcomes of clinical care along with reduction in costs. Near-infrared, surface-enhanced fluorescence (NIR-SEF) is a promising approach to improve assay sensitivity via simultaneous increase in signal with a reduction in background. This dissertation describes research conducted with the overall goal to determine the extent to which fluorescence in solution may be enhanced by altering specific variables involved in the formation of plasmonactive nanostructures of dye-labeled protein and silver nanoparticles ...


Selective Detection And Automated Counting Of Fluorescently-Labeled Chrysotile Asbestos Using A Dual-Mode High-Throughput Microscopy (Dm-Htm) Method, Myoung-Ock Cho, Hyo Mi Chang, Donghee Lee, Yeon Gyu Yu, Hwataik Han, Jung Kyung Kim Jan 2013

Selective Detection And Automated Counting Of Fluorescently-Labeled Chrysotile Asbestos Using A Dual-Mode High-Throughput Microscopy (Dm-Htm) Method, Myoung-Ock Cho, Hyo Mi Chang, Donghee Lee, Yeon Gyu Yu, Hwataik Han, Jung Kyung Kim

Mechanical & Materials Engineering Faculty Publications

Phase contrast microscopy (PCM) is a widely used analytical method for airborne asbestos, but it is unable to distinguish asbestos from non-asbestos fibers and requires time-consuming and laborious manual counting of fibers. Previously, we developed a high-throughput microscopy (HTM) method that could greatly reduce human intervention and analysis time through automated image acquisition and counting of fibers. In this study, we designed a dual-mode HTM (DM-HTM) device for the combined reflection and fluorescence imaging of asbestos, and automated a series of built-in image processing commands of ImageJ software to test its capabilities. We used DksA, a chrysotile-adhesive protein, for selective ...