Open Access. Powered by Scholars. Published by Universities.®

Nanoscience and Nanotechnology Commons

Open Access. Powered by Scholars. Published by Universities.®

2013

Series

University of Massachusetts Medical School

Articles 1 - 1 of 1

Full-Text Articles in Nanoscience and Nanotechnology

Stem Cell Labeling Using Polyethylenimine Conjugated (Alpha-Naybf4:Tm3+)/Caf2 Upconversion Nanoparticles, Liang Zhao, Artem Kutikov, Jie Shen, Chunying Duan, Jie Song, Gang Han Mar 2013

Stem Cell Labeling Using Polyethylenimine Conjugated (Alpha-Naybf4:Tm3+)/Caf2 Upconversion Nanoparticles, Liang Zhao, Artem Kutikov, Jie Shen, Chunying Duan, Jie Song, Gang Han

University of Massachusetts Medical School Faculty Publications

We report on a polyethylenimine (PEI) covalently conjugated (alpha-NaYbF4:Tm3+)/CaF2 upconversion nanoparticle (PEI-UCNP) and its use for labeling rat mesenchymal stem cells (rMSCs). The PEI-UCNPs absorb and emit near-infrared light, allowing for improved in vivo imaging depth over conventional probes. We found that such covalent surface conjugation by PEI results in a much more stable PEI-UCNP suspension in PBS compared to conventional electrostatic layer by layer (LbL) self-assembling coating approach. We systematically examined the effects of nanoparticle dose and exposure time on rat mesenchymal stem cell (rMSC) cytotoxicity. The exocytosis of PEI-UCNPs from labeled rMSCs and the impact of ...