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Nanoscience and Nanotechnology Commons

Open Access. Powered by Scholars. Published by Universities.®

2010

University of South Carolina

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Full-Text Articles in Nanoscience and Nanotechnology

Far-Field Optical Nanoscopy Based On Continuous Wave Laser Stimulated Emission Depletion, C. Kuang, Wei Zhao, Guiren Wang Jan 2010

Far-Field Optical Nanoscopy Based On Continuous Wave Laser Stimulated Emission Depletion, C. Kuang, Wei Zhao, Guiren Wang

Faculty Publications

Stimulated emission depletion (STED) microscopy is one of the breakthrough technologies that belong to far-field optical microscopy and can achieve nanoscale spatial resolution. We demonstrate a far-field optical nanoscopy based on continuous wave lasers with different wavelengths, i.e., violet and green lasers for excitation and STED, respectively. Fluorescent dyes Coumarin 102 and Atto 390 are used for validating the depletion efficiency. Fluorescent nanoparticles are selected for characterizing the spatial resolution of the STED system. Linear scanning of the laser beams of the STED system along one line of a microscope slide, which is coated with the nanoparticles, indicates that ...