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Synthesis And Functionalization Of Gold Nanoparticles Using Chemically Modified Ssdna, Philip Gerard Calabrese
Synthesis And Functionalization Of Gold Nanoparticles Using Chemically Modified Ssdna, Philip Gerard Calabrese
Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)
In the first part of this thesis, methods for functionalizing spherical gold nanoparticles with nucleic acid binding ligands (aptamers) that target the VEGF receptor complex were developed. In order to provide a multiplexed labeling strategy for imaging the VEGF receptor complex in electron microscopy, gold nanoparticles of distinct sizes were conjugated to modified ssDNA aptamers that target the VEGF-A cytokine, the VEGFR-2 RTK receptor and a membrane associated co-receptor, Nrp-1. The modified ssDNA gold nanoparticle conjugates were applied to a human lung carcinoma cell line (A549) which has been shown to express each of these proteins and used as a ...
Rna Mediated Assembly Of Nanostructures, Jessica Lynn Rouge
Rna Mediated Assembly Of Nanostructures, Jessica Lynn Rouge
Chemistry & Biochemistry Graduate Theses & Dissertations (1986-2018)
The first chapter of this work presents a comprehensive look at RNA mediated nanoparticle formation. The overall goal of this research is to gain a deeper understanding of the RNA-particle formation mechanism and the basic properties of the materials selected by modified RNA molecules. Understanding such RNA-substrate interactions and how they translate into the physical and chemical characteristics of the nanoparticles they create are important fundamental concepts when considering these biotemplated materials as potential chemical catalysts. The RNA sequences discussed in the first chapter (referred to as Pdases) were discovered using RNA in vitro selection techniques. These Pdases were found ...