Nanoscience and Nanotechnology Commons^™

Bio Roll-Up: Self-Assembling Scaffolds For Tissue Engineering, Chase Austyn Brisbois

Nanoscale Science & Engineering (discontinued with class year 2014)

There is an unmet need for controlled, dynamic cell scaffolds that will generate 3-D monolayers of epithelial cells for medical research and tissue engineering. Here we describe a system that uses photoimageable and biocompatible HEMA-based hydrogel bilayers that self-assemble from 2-D substrates into 3-D tube-shaped structures. These structures are patterned on top of another layer that demonstrates an ability to anchor the assembled structure and control the direction and extent of selfassembly. Cell viability was determined by MTT assay which shows material components comparable with the positive control (80-110%). Finally, the masks used in the experiments herein were redesigned to …