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Full-Text Articles in Nanoscience and Nanotechnology
Resistive Pulse Sensing Of Protein Unfolding And Transport In Solid-State Nanopores, Jugal Saharia
Resistive Pulse Sensing Of Protein Unfolding And Transport In Solid-State Nanopores, Jugal Saharia
Mechanical Engineering Research Theses and Dissertations
Solid-state nanopore sensors have attracted considerable attraction as a tool for solution-based single-molecule studies and have been successfully utilized for characterization of biomolecules such as nucleic acids, proteins, glycans, viruses, etc. Among these, characterization of proteins has been more challenging due to their charge heterogeneity and the complex energy landscape associated with different protein conformations. Presented in this thesis is the fabrication of solid-state nanopores and their application for characterizing proteins and understanding their transport through nanopores. Fabrication of nanometer-sized pores in SixNy membranes was achieved using the conventional controlled dielectric breakdown method as well as a …
Microparticle Propulsion For In Vivo Navigation, Louis Rogowski
Microparticle Propulsion For In Vivo Navigation, Louis Rogowski
Mechanical Engineering Research Theses and Dissertations
Microscale propulsion impacts a diverse array of fields, with simplistic microrobots allowing for novel innovations in microscale surgery and drug delivery. Propulsion at the microscale is constrained by physics, with time-reversal and geometric symmetries limiting available propulsion mechanisms. However, certain fluid environments and surface coatings allow for the propulsion of microparticles through externally applied magnetic fields. Presented here is a detailed analysis of microparticles propelling using spontaneous symmetry breaking, flagella surface coatings, and multi-modal actuation mechanisms. Spontaneous symmetry breaking in nonlinearly viscoelastic fluids is presented for the first time in literature, with two equal and opposite propulsion states existing along …
Soft-Microrobotics: The Manipulation Of Alginate Artificial Cells, Samuel Sheckman
Soft-Microrobotics: The Manipulation Of Alginate Artificial Cells, Samuel Sheckman
Mechanical Engineering Research Theses and Dissertations
In this work, the approach to the manipulation of alginate artificial cell soft-microrobots, both individually and in swarms is shown. Fabrication of these artificial cells were completed through centrifugation, producing large volumes of artificial cells, encapsulated with superparamagnetic iron oxide nanoparticles; these artificial cells can be then externally stimulated by an applied magnetic field. The construction of a Permeant Magnet Stage (PMS) was produced to manipulate the artificial cells individually and in swarms. The stage functionalizes the permanent magnet in the 2D xy-plane. Once the PMS was completed, Parallel self-assembly (Object Particle Computation) using swarms of artificial cells in complex …
Multiple Consecutive Recapture Of Rigid Nanoparticles Using A Solid-State Nanopore Sensor, Jungsoo Lee
Multiple Consecutive Recapture Of Rigid Nanoparticles Using A Solid-State Nanopore Sensor, Jungsoo Lee
Mechanical Engineering Research Theses and Dissertations
Solid‐state nanopore sensors have been used to measure the size of a nanoparticle by applying a resistive pulse sensing technique. Previously, the size distribution of the population pool could be investigated utilizing data from a single translocation, however, the accuracy of the distribution is limited due to the lack of repeated data. In this study, we characterized polystyrene nanobeads utilizing single particle recapture techniques, which provide a better statistical estimate of the size distribution than that of single sampling techniques. The pulses and translocation times of two different sized nanobeads (80 nm and 125 nm in diameter) were acquired repeatedly …