Nanoscience and Nanotechnology Commons^™

Exploitation And Exploration Of Pcr In Microfluidic Systems With Gradient Temperature Environments, Ilija Pjescic

Doctoral Dissertations

The main goal of the work was to establish a wholesome picture off all relevant processes for a sample-in, answer out genetic system and to integrate the whole process on a one step device from sample collection to final result. The genetic analysis process consists of ideally three steps: sample preparation, chemical reaction, and analysis. Each of the steps is different and requires a specific environment, where sample preparation might use additives, they might later interfere with the reaction itself or lead to misleading results in the analysis phase. It was found to be quite a challenging process to synchronize …