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Mechanical Engineering

Mechanical Engineering Publications

Aptamer

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Actuation Of Chitosan-Aptamer Nanobrush Borders For Pathogen Sensing, Katherine D. Hills, Daniela Alves De Oliveira, Nicholas D. Cavallaro, Carmen L. Gomes, Eric S. Mclamore Jan 2018

Actuation Of Chitosan-Aptamer Nanobrush Borders For Pathogen Sensing, Katherine D. Hills, Daniela Alves De Oliveira, Nicholas D. Cavallaro, Carmen L. Gomes, Eric S. Mclamore

Mechanical Engineering Publications

We demonstrate a sensing mechanism for rapid detection of Listeria monocytogenes in food samples using the actuation of chitosan-aptamer nanobrush borders. The bio-inspired soft material and sensing strategy mimic natural symbiotic systems, where low levels of bacteria are selectively captured from complex matrices. To engineer this biomimetic system, we first develop reduced graphene oxide/nanoplatinum (rGO-nPt) electrodes, and characterize the fundamental electrochemical behavior in the presence and absence of chitosan nanobrushes during actuation (pH-stimulated osmotic swelling). We then characterize the electrochemical behavior of the nanobrush when receptors (antibodies or DNA aptamers) are conjugated to the surface. Finally, we test various ...