Mechanical Engineering Commons^™

Freezing Processes In Cell Suspensions Evaluated Using Cryomicroscopy, Tathagata Acharya

LSU Master's Theses

This thesis aims at evaluating the freezing response of three different cell types, Pacific Oyster embryos, Jurkats and Helas, using the technique of cryomicroscopy. The choice of cells was primarily based on supporting ongoing research work at the Bioengineering Laboratory, Department of Mechanical Engineering at Louisiana State University in Baton Rouge. On a secondary basis, the cells were chosen based on their contrasting nature. While Pacific Oyster being a favorite food in USA calls for successful techniques of cryopreservation of their embryos in order to keep up with the growing demand, Jurkat and HeLa are undesired malignant human cells that …