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2008

California Polytechnic State University, San Luis Obispo

Articular cartilage; Growth and remodeling; Cartilage matrix; Glycosaminoglycan; Collagen crosslinks; ?-Aminopropionitrile

Articles 1 - 1 of 1

Full-Text Articles in Mechanical Engineering

Cartilage Growth And Remodeling: Modulation Of Balance Between Proteoglycan And Collagen Network In Vitro With Β-Aminopropionitrile, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah Jan 2008

Cartilage Growth And Remodeling: Modulation Of Balance Between Proteoglycan And Collagen Network In Vitro With Β-Aminopropionitrile, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah

Mechanical Engineering

Objective

To examine the effect of β-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro.

Design

Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed.

Results

The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in ...