Mechanical Engineering Commons^™

A Nonlinear Finite Element Model Of Cartilage Growth, Andrew Davol, Michael S. Bingham, Robert L. Sah, Stephen M. Klisch

Mechanical Engineering

The long range objective of this work is to develop a cartilage growth finite element model (CGFEM), based on the theories of growing mixtures that has the capability to depict the evolution of the anisotropic and inhomogeneous mechanical properties, residual stresses, and nonhomogeneities that are attained by native adult cartilage. The CGFEM developed here simulates isotropic in vitro growth of cartilage with and without mechanical stimulation. To accomplish this analysis a commercial finite element code (ABAQUS) is combined with an external program (MATLAB) to solve an incremental equilibrium boundary value problem representing one increment of growth. This procedure is repeated …

Regulation Of Immature Cartilage Growth By Igf-I, Tgf- Β 1, Bmp-7, And Pdgf-Ab: Role Of Metabolic Balance Between Fixed Charge And Collagen Network, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah

Mechanical Engineering

Cartilage growth may involve alterations in the balance between the swelling tendency of proteoglycans and the restraining function of the collagen network. Growth factors, including IGF-I, TGF-β1, BMP-7, and PDGF-AB, regulate chondrocyte metabolism and, consequently, may regulate cartilage growth. Immature bovine articular cartilage explants from the superficial and middle zones were incubated for 13 days in basal medium or medium supplemented with serum, IGF-I, TGF-β1, BMP-7, or PDGF-AB. Variations in tissue size, accumulation of proteoglycan and collagen, and tensile properties were assessed. The inclusion of serum, IGF-I, or BMP-7 resulted in expansive tissue growth, stimulation of proteoglycan deposition but not …

Articular Cartilage Tensile Integrity: Modulation By Matrix Depletion Is Maturation-Dependent, Anna Asanbaeva, Johnny Tam, Barbara L. Schumacher, Stephen M. Klisch, Koichi Masuda, Robert L. Sah

Mechanical Engineering

Articular cartilage function depends on the molecular composition and structure of its extracellular matrix (ECM). The collagen network (CN) provides cartilage with tensile integrity, but must also remodel during growth. Such remodeling may depend on matrix molecules interacting with the CN to modulate the tensile behavior of cartilage. The objective of this study was to determine the effects of increasingly selective matrix depletion on tensile properties of immature and mature articular cartilage, and thereby establish a framework for identifying molecules involved in CN remodeling. Depletion of immature cartilage with guanidine, chondroitinase ABC, chondroitinase AC, and Streptomyces hyaluronidase markedly increased tensile …

A Cartilage Growth Mixture Model With Collagen Remodeling: Validation Protocols, Stephen M. Klisch, Anna Asanbaeva, Sevan R. Oungoulian, Koichi Masuda, Eugene J.-Ma. Thonar, Andrew Davol, Robert L. Sah

Mechanical Engineering

A cartilage growth mixture (CGM) model is proposed to address limitations of a model used in a previous study. New stress constitutive equations for the solid matrix are derived and collagen (COL) remodeling is incorporated into the CGM model by allowing the intrinsic COL material constants to evolve during growth. An analytical validation protocol based on experimental data from a recent in vitro growth study is developed. Available data included measurements of tissue volume, biochemical composition, and tensile modulus for bovine calf articular cartilage (AC) explants harvested at three depths and incubated for 13 days in 20% fetal borine serum …

Bioengineering Cartilage Growth, Maturation, And Form, Gregory M. Williams, Stephen M. Klisch, Robert L. Sah

Mechanical Engineering

Cartilage of articular joints grows and matures to achieve characteristic sizes, forms, and functional properties. Through these processes, the tissue not only serves as a template for bone growth but also yields mature articular cartilage providing joints with a low-friction, wear-resistant bearing material. The study of cartilage growth and maturation is a focus of both cartilage biologists and bioengineers with one goal of trying to create biologic tissue substitutes for the repair of damaged joints. Experimental approaches both in vivo and in vitro are being used to better understand the mechanisms and regulation of growth and maturation processes. This knowledge …

Nonlinear Contact Analysis Of Gear Teeth For Malfunction Diagnostics, D. Kong, J. M. Meagher, C. Xu, X. Wu, Y. Wu

Mechanical Engineering

Gearboxes sustain a variety of faults such as broken-shafts, eroded, broken, or missing teeth, and even broken-cases. Casing mounted accelerometers can detect fault patterns but the signals are complicated and difficult to interpret. This study considers the tooth loading of ideal gears and gears with defects. A large industrial gearbox used in a 12m³ electric mining shovel is modeled. The nonlinear contact mechanics is analyzed to predict the bearing supporting force variation vs. the gear tooth loading after a 3-D CAD model of the gearbox is transferred into multi-body dynamics software. The contact mechanics model of the meshing teeth …

Cartilage Growth And Remodeling: Modulation Of Balance Between Proteoglycan And Collagen Network In Vitro With Β-Aminopropionitrile, Anna Asanbaeva, Koichi Masuda, Eugene J-M.A. Thonar, Stephen M. Klisch, Robert L. Sah

Mechanical Engineering

Objective

To examine the effect of β-aminopropionitrile (BAPN), an inhibitor of lysyl oxidase, on growth and remodeling of immature articular cartilage in vitro.

Design

Immature bovine articular cartilage explants from the superficial and middle layers were cultured for 13 days in serum-containing medium with or without BAPN. Variations in tissue size, accumulation of proteoglycan and collagen (COL), and tensile mechanical properties were assessed.

Results

The inclusion of serum resulted in expansive tissue growth, stimulation of proteoglycan and COL deposition, and a diminution of tensile integrity. Supplementation of medium with BAPN accentuated this phenotype in terms of a further increase in …

Cal Poly Wind Power Research Center, Patrick Lemieux, John Ridgely

Mechanical Engineering

The Wind Power Research Center is being created at California State Polytechnic University in San Luis Obispo, California. This center brings together faculty, undergraduate and graduate students, and industry partners to conduct applied research and education in support of the wind power industry. Particular emphasis is given to research which serves the needs of manufacturers of smaller wind turbines, and to educating engineers who are well trained to work in all segments of the wind power industry.

A Two-Disk Extended Jeffcott Rotor Model Distinguishing A Shaft Crack From Other Rotating Asymmetries, Xi Wu, Jim Meagher

Mechanical Engineering

A mathematical model of a cracked rotor and an asymmetric rotor with two disks representing a turbine and a generator is utilized to study the vibrations due to imbalance and side load. Nonlinearities typically related with a “breathing” crack are included using a Mayes steering function. Numerical simulations demonstrate how the variations of rotor parameters affect the vibration response and the effect of coupling between torsional and lateral modes. Bode, spectrum, and orbit plots are used to show the differences between the vibration signatures associated with cracked shafts versus asymmetric shafts. Results show how nonlinear lateral-torsional coupling shifts the resonance …

Response Of A Warped Flexible Rotor With A Fluid Bearing, Jim Meagher, Xi Wu, Chris Lencioni

Mechanical Engineering

A two-complex-degrees-of-freedom model is developed and compared to experimental data for various amounts of rotor bow and its orientation to mass imbalance of the rotor. The equation of motion is developed by adding constant forces that rotate with the rotor to a Bently-Muszynska two-mode isotropic rotor model with a plane journal bearing. Diagnostic information discernable from probes at the bearing is explored and compared to midspan response, where previous research has concentrated. The model presented also extends earlier work by representing the effect of a nonrigid bearing. Good agreement between the analytical model and experiment demonstrates that the analysis presented …