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Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Novel Quantitative Macro Biomolecule Analysis Based On A Micro Coulter Counter, Y Han, H. Wu, F. Liu, Gang Cheng, Jiang Zhe
Dr. Jiang Zhe
We demonstrate quantitative biomolecule analysis using a micro coulter counter. Specific binding between antibody functionalized microparticles and target biomolecule cause large aggregates of microparticles. The micro coulter counter was employed to measure aggregation ratio, ratio of the aggregate counts to the total particle counts. Goat anti-rabbit IgG, used as a model biomarker, were tested in this paper. The experiment results showed that the aggregation ratio increases with the increasing biomolecule concentration, and the detectable concentration range from 16 to 160 ng/ml was achieved.