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Full-Text Articles in Mechanical Engineering

Parametric Nasopharyngeal Swab For Sampling Covid-19 And Other Respiratory Viruses: Open Source Design, Sla 3-D Printing And Uv Curing System, Nicole Gallup, Adam Pringle, Shane Oberloier, Nagendra Gautam Tanikella, Joshua M. Pearce May 2020

Parametric Nasopharyngeal Swab For Sampling Covid-19 And Other Respiratory Viruses: Open Source Design, Sla 3-D Printing And Uv Curing System, Nicole Gallup, Adam Pringle, Shane Oberloier, Nagendra Gautam Tanikella, Joshua M. Pearce

Michigan Tech Publications

Access to nasopharyngeal swabs for sampling remain a bottleneck in some regions for COVID19 testing. This study develops a distributed manufacturing solution using only an open source manufacturing tool chain consisting of two types of open source 3-D printing and batch UV curing, and provides a parametric fully free design of a nasopharyngeal swab. The swab was designed using parametric OpenSCAD in two components (a head with engineered break point and various handles), which has several advantages: i) minimizing print time on relatively slow SLA printers, ii) enabling the use of smaller print volume open source SLA printers, iii) reducing ...


Tra-1-60-Positive/Cd45low Cells Found In The Peripheral Blood Of Prostate Cancer Patients With Metastatic Disease – A Proof-Of-Concept Study, Claudia Schäfer, Yawen Ju, Youngbin Tak, Cesar Vazquez, Sangyoon J. Han, Edwin Tan, Et Al. Jan 2020

Tra-1-60-Positive/Cd45low Cells Found In The Peripheral Blood Of Prostate Cancer Patients With Metastatic Disease – A Proof-Of-Concept Study, Claudia Schäfer, Yawen Ju, Youngbin Tak, Cesar Vazquez, Sangyoon J. Han, Edwin Tan, Et Al.

Michigan Tech Publications

Purpose

Over 90% of all cancer related deaths are due to metastasis. However, current diagnostic tools can't reliably discriminate between invasive and localized cancers.

Patients and methods

In this proof-of-concept study, we employed the embryonic stem cell marker TRA-1-60 (TRA+) to identify TRA + cells within the blood of prostate cancer patients and searched for TRA + cells in men with metastatic and localized cancers. We isolated whole peripheral blood mononuclear cells from 26 metastatic prostate cancer patients, from 13 patients with localized prostate cancer and from 17 healthy controls. Cells were stained for DAPI, CD45 and TRA + by immunofluorescence and ...