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Full-Text Articles in Engineering Science and Materials

Mimicking The Arterial Microenvironment With Peg-Pc To Investigate The Roles Of Physicochemical Stimuli In Smc Phenotype And Behavior, William G. Herrick Jan 2015

Mimicking The Arterial Microenvironment With Peg-Pc To Investigate The Roles Of Physicochemical Stimuli In Smc Phenotype And Behavior, William G. Herrick

Doctoral Dissertations

The goal of this dissertation was to parse the roles of physical, mechanical and chemical cues in the phenotype plasticity of smooth muscle cells (SMCs) in atherosclerosis. We first developed and characterized a novel synthetic hydrogel with desirable traits for studying mechanotransduction in vitro. This hydrogel, PEG-PC, is a co-polymer of poly(ethylene glycol) and phosphorylcholine with an incredible range of Young’s moduli (~1 kPa - 9 MPa) that enables reproduction of nearly any tissue stiffness, exceptional optical and anti-fouling properties, and support for covalent attachment of extracellular matrix (ECM) proteins. To our knowledge, this combination of mechanical range, low ...


Quantification Of Plaque Stiffness By Brillouin Microscopy In Experimental Thin Cap Fibroatheroma, Giuseppe Antonacci, Ryan M. Pedrigi, Avinash Kondiboyina, Vikram V. Mehta, Ranil De Silva, Carl Paterson, Rob Krams, Peter Torok Jan 2015

Quantification Of Plaque Stiffness By Brillouin Microscopy In Experimental Thin Cap Fibroatheroma, Giuseppe Antonacci, Ryan M. Pedrigi, Avinash Kondiboyina, Vikram V. Mehta, Ranil De Silva, Carl Paterson, Rob Krams, Peter Torok

Mechanical & Materials Engineering Faculty Publications

Plaques vulnerable to rupture are characterized by a thin and stiff fibrous cap overlaying a soft lipid-rich necrotic core. The ability to measure local plaque stiffness directly to quantify plaque stress and predict rupture potential would be very attractive, but no current technology does so. This study seeks to validate the use of Brillouin microscopy to measure the Brillouin frequency shift, which is related to stiffness, within vulnerable plaques. The left carotid artery of an ApoE-/- mouse was instrumented with a cuff that induced vulnerable plaque development in nine weeks. Adjacent histological sections from the instrumented and control arteries were ...