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Full-Text Articles in Biological Engineering

Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen Jan 2018

Efficient One‑Step Fusion Pcr Based On Dual‑Asymmetric Primers And Two‑Step Annealing, Yilan Liu, Jinjin Chen, Anders Thygesen

Chemical and Biomolecular Engineering -- All Faculty Papers

Gene splicing by fusion PCR is a versatile and widely used methodology, especially in synthetic biology. We here describe a rapid method for splicing two fragments by one-round fusion PCR with a dual-asymmetric primers and two-step annealing (ODT) method. During the process, the asymmetric intermediate fragments were generated in the early stage. Thereafter, they were hybridized in the subsequent cycles to serve as template for the target full-length product. The process parameters such as primer ratio, elongation temperature and cycle numbers were optimized. In addition, the fusion products produced with this method were successfully applied in seamless genome editing. The ...


Recovery And Utilization Of Lignin Monomers As Part Of The Biorefinery Approach, Kirsten M. Davis, Marjorie Rover, Robert C. Brown, Xianglan Bai, Zhiyou Wen, Laura R. Jarboe Dec 2017

Recovery And Utilization Of Lignin Monomers As Part Of The Biorefinery Approach, Kirsten M. Davis, Marjorie Rover, Robert C. Brown, Xianglan Bai, Zhiyou Wen, Laura R. Jarboe

Robert C. Brown

Lignin is a substantial component of lignocellulosic biomass but is under-utilized relative to the cellulose and hemicellulose components. Historically, lignin has been burned as a source of process heat, but this heat is usually in excess of the process energy demands. Current models indicate that development of an economically competitive biorefinery system requires adding value to lignin beyond process heat. This addition of value, also known as lignin valorization, requires economically viable processes for separating the lignin from the other biomass components, depolymerizing the lignin into monomeric subunits, and then upgrading these monomers to a value-added product. The fact that ...


Responsive Pentablock Copolymers For Sirna Delivery, Surya K. Mallapragada, Metin Uz, Sacide Alsoy Altinkaya Dec 2017

Responsive Pentablock Copolymers For Sirna Delivery, Surya K. Mallapragada, Metin Uz, Sacide Alsoy Altinkaya

Surya K. Mallapragada

In this study, temperature and pH responsive cationic and amphiphilic pentablock copolymers, which consist of the temperature responsive triblock Pluronic F127 sandwiched between pH responsive PDEAEM (poly(2-diethylaminoethyl methacrylate)) end blocks, were used for the first time in the development of polyplex and gold nanoparticle (AuNP) based multicomponent siRNA delivery systems (MCSs). Copolymers in both systems protected siRNA from external effects, provided cell entry and endosomal escape. The thermoreversible micellization of the hydrophobic PPO block facilitated the cellular entry while the PDEAEM blocks enhanced the endosomal escape through protonated tertiary amine groups by pH buffering. The synergistic advantages of the ...


Characterization Of Sheared Colloidal Aggregation Using Langevin Dynamics Simulation, Sergiy Markutsya, Rodney O. Fox, Shankar Subramaniam Dec 2017

Characterization Of Sheared Colloidal Aggregation Using Langevin Dynamics Simulation, Sergiy Markutsya, Rodney O. Fox, Shankar Subramaniam

Rodney O. Fox

Aggregation of colloidal particles under shear is studied in model systems using a Langevin dynamics model with an improved interparticle interaction potential. In the absence of shear, aggregates that form are characterized by compact structure at small scales and ramified structure at larger scales. This confirms the structural crossover mechanism previously suggested by Sorensen and coworkers, that colloidal aggregation occurs due to monomer addition at small scales and due to cluster-cluster aggregation at large scales. The fractal dimension of nonsheared aggregates is scale-dependent. Smaller aggregates have a higher fractal dimension than larger ones, but the radius of gyration where this ...


Investigation And Engineering Of Polyketide Biosynthetic Pathways, Lei Sun Dec 2017

Investigation And Engineering Of Polyketide Biosynthetic Pathways, Lei Sun

All Graduate Theses and Dissertations

This research is focused on investigation and engineering of natural product biosynthetic pathways for efficient production of pharmaceutically important molecules or generation of new bioactive molecules for drug development.

Natural products are an important source of therapeutics, such as chromomycin (anti-cancer), emodin (anti-inflammatory and anti-tumor) and sprolaxine (anti-Helicobacter pylori). Metabolic engineering of natural product biosynthetic pathways shows its promise for creating and producing valuable compounds with chemical diversity for drug discovery. One goal of this research is to create highly efficient strains to biosynthesize valuable natural products. The engineered Streptomyces roseiscleroticus strain constructed in this work showed higher titers of ...


Gender And Participation In An Engineering Problem-Based Learning Environment, Laura Hirshfield, Milo D. Koretsky Nov 2017

Gender And Participation In An Engineering Problem-Based Learning Environment, Laura Hirshfield, Milo D. Koretsky

Interdisciplinary Journal of Problem-Based Learning

The use of problem-based learning (PBL) is gaining attention in the engineering classroom as a way to help students synthesize foundational knowledge and to better prepare students for practice. In this work, we study the discourse interactions between 27 student teams and two instructors in an engineering PBL environment to analyze how participation is distributed among team members, paying particular attention to the differences between male and female students. There were no statistically significant differences between the amount that male and female students spoke; however, stereotypical gender roles and traditional gendered behavior did manifest in the discussion. Also, regardless of ...


Escherichia Coli Attachment To Model Particulates: The Effects Of Bacterial Cell Characteristics And Particulate Properties, Xiao Liang, Chunyu Liao, Michelle L. Soupir, Laura R. Jarboe, Michael L. Thompson, Philip M. Dixon Sep 2017

Escherichia Coli Attachment To Model Particulates: The Effects Of Bacterial Cell Characteristics And Particulate Properties, Xiao Liang, Chunyu Liao, Michelle L. Soupir, Laura R. Jarboe, Michael L. Thompson, Philip M. Dixon

Chemical and Biological Engineering Publications

E. coli bacteria move in streams freely in a planktonic state or attached to suspended particulates. Attachment is a dynamic process, and the fraction of attached microorganisms is thought to be affected by both bacterial characteristics and particulate properties. In this study, we investigated how the properties of cell surfaces and stream particulates influence attachment. Attachment assays were conducted for 77 E. coli strains and three model particulates (ferrihydrite, Ca-montmorillonite, or corn stover) under environmentally relevant conditions. Surface area, particle size distribution, and total carbon content were determined for each type of particulate. Among the three particulates, attachment fractions to ...


Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos Sep 2017

Influence Of Fibroblasts On Functional Arteriogenesis In A Murine Chronic Hindlimb Ischemia Model, Ashli A. Santos

Biomedical Eng/General Eng.

Peripheral arterial occlusive disease (PAOD) occurs when there is a narrowing or blockage – usually a buildup of plaque - within the arteries that reduces blood flow to tissues which can chronic ischemia. As with most diseases, early detection and proactive treatment are important to maximize prognosis. Exercise effectively treats PAOD, but due to ischemic pain in the limbs, or intermittent claudication (IC), exercise can become painful and difficult. Due to the buildup of plaque, occlusions create an ischemic environment that changes the pressure distribution in collateral networks and increases the shear stress in transverse collaterals. Those two responses signal the beginning ...


The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco Aug 2017

The Subject Librarian Newsletter, Engineering And Computer Science, Fall 2017, Buenaventura "Ven" Basco

Libraries' Newsletters

No abstract provided.


Rice Hull Bioreactor For Recirculating Aquaculture, Marlon A. Greensword Aug 2017

Rice Hull Bioreactor For Recirculating Aquaculture, Marlon A. Greensword

LSU Doctoral Dissertations

The engineering of floating media biofilters has been optimized over the years. The backwashing process has made them more energy and water efficient. Likewise, moving bed bioreactors (MBBR) are gaining interest and popularity because they are relatively affordable to build. Yet, developing countries’ aquaculture production remains largely excluded from the advances made in recirculating aquaculture systems (RAS). This discrepancy is partially driven by the high costs of media such plastic beads and Kaldnes (KMT) media, commonly used in MBBR.

This dissertation evaluates the usability and profitability of rice hulls (RH), an abundant by-product in many developing nations, as a sinking ...


Fundamental Characterization Of Oxygen Nanobubbles, John Hamlin, Yi Wen, Joseph Irudayaraj Aug 2017

Fundamental Characterization Of Oxygen Nanobubbles, John Hamlin, Yi Wen, Joseph Irudayaraj

The Summer Undergraduate Research Fellowship (SURF) Symposium

A hypoxic environment is created by tumors’ incredible growth rate. Hypoxia provides radioresistance to the tumors, thus making radiation treatment less effective. The issue is that increasing the radiation leads to increased side effects in patients. Our goal for the oxygen-filled nanobubble is to deliver oxygen to the tumor to lessen radioresistance and make radiation treatment more efficient. However, we need preliminary research to understand and improve the nanobubbles before further research and implementation. To do this, we synthesized different batches of nanobubbles to optimize the production method and find the best container and temperature to store nanobubbles. We measured ...


The Response Of Schwann Cells To Weak Dc Electric Fields, Alexander T. Lai, Jianming Li Aug 2017

The Response Of Schwann Cells To Weak Dc Electric Fields, Alexander T. Lai, Jianming Li

The Summer Undergraduate Research Fellowship (SURF) Symposium

Schwann cells are glial cells that serve the vital role of supporting neurons in the peripheral nervous system. While their primary function is to provide insulation (myelin) for axons, they also help regenerate injured axons by digesting severed axons and providing scaffolding to guide the regeneration process. This specific role of Schwann cells makes them highly important cellular targets following nerve injury. Although some efforts have been made to encourage Schwann cell migration after nerve damage, the use of electric fields to control cell responses remain unexplored; therefore, this experiment serves to characterize the behavior of Schwann cells to weak ...


Pathogenic Dna Detection Using Dna Hairpins: A Non-Linear Hybridization Chain Reaction Platform, Lance Novak, Tamara L. Kinzer-Ursem Aug 2017

Pathogenic Dna Detection Using Dna Hairpins: A Non-Linear Hybridization Chain Reaction Platform, Lance Novak, Tamara L. Kinzer-Ursem

The Summer Undergraduate Research Fellowship (SURF) Symposium

Currently, 3.2 billion people are at risk of being infected with malaria, with 1.2 billion of those being at high risk (>1 in 1000 chance of getting malaria in a year). Thus, there is a need for a biosensor that is highly sensitive, cost effective, and simple to use for point-of-care diagnosis. The biosensing platform, PathVis, has achieved this by measuring changes in fluid properties after a loop-mediated isothermal amplification (LAMP). LAMP is a DNA amplification system that requires enzymes and a temperature of 65degrees C. LAMP currently limits PathVis by being costly, requiring refrigeration, and difficult to ...


Localized Blood Occlusion Generation In An In-Vitro Circulatory Catheter System, Ryan D. Harris, Qi Yang, Hyowon Lee Aug 2017

Localized Blood Occlusion Generation In An In-Vitro Circulatory Catheter System, Ryan D. Harris, Qi Yang, Hyowon Lee

The Summer Undergraduate Research Fellowship (SURF) Symposium

Hydrocephalus is a debilitating neurological disorder that involves the accumulation of cerebrospinal fluid in a ventricle of the brain. The implantation of a catheter commonly treats hydrocephalus with drainage. These catheters have a short lifespan due to obstruction from biological materials. Shunt systems have an extremely high failure rate of more than 40% failed within 1 year and up to 85% failed within 10 years. Previously, polymer-based flexible implantable magnetic micro-actuators were developed to clean up the catheter by mechanical vibration. We have demonstrated clearing of bacteria attachment and are proceeding to examine clearing effects on larger clotting materials, such ...


Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus Aug 2017

Establishing A Lung Model For Evaluation Of Engineered Lung Microbiome Therapies, Kathryn F. Atherton, Stephen Miloro, Jenna Rickus

The Summer Undergraduate Research Fellowship (SURF) Symposium

Benzene, a toxin and carcinogen found in air polluted by cigarette smoke, car exhaust, and industrial processes, is associated with the development of leukemia and lymphoma. Other than avoiding exposure, there is no current method to deter the effects of benzene. One potential strategy to prevent these effects is to engineer the bacteria of the human lung microbiome to degrade benzene. To evaluate this novel approach, we must verify that the bacteria remain viable within the lung microenvironment. To do so, lungs were harvested from rats and swabbed to determine the contents of the original lung microbiome. Then green fluorescent ...


Evaluating Thermal Comfort Of Broiler Chickens During Transportation Using Heat Index And Simulated Electronic Chickens, Kaushik Luthra Aug 2017

Evaluating Thermal Comfort Of Broiler Chickens During Transportation Using Heat Index And Simulated Electronic Chickens, Kaushik Luthra

Theses and Dissertations

Broilers experience high physiological stress during pre-slaughter transport, especially under extremes of thermal environment. Characterization of thermal environment on the trailer is crucial to identify stress-prone regions during transportation. At the same time, Broilers experience high physiological stress during pre-slaughter transport, especially under extremes of thermal environment. Characterization of thermal environment on the trailer is crucial to identify stress-prone regions during transportation. At the same time, quantification of heat loss of the broilers loaded on trailers is important in understanding the well-being of the broilers. We have developed four electronic chickens (E-chickens) to simulate the sensible heat loss of live ...


An Automated Device To Increase Screening Throughput Of Zebrafish Larvae, Fuoad Saliou-Sulley Aug 2017

An Automated Device To Increase Screening Throughput Of Zebrafish Larvae, Fuoad Saliou-Sulley

Electronic Theses and Dissertations

The use of the zebrafish as an animal model alternative to mammalian species has spawned research advancements in several medical fields. Since the zebrafish shares a high degree of sequence and functional homology with mammals, studies using this organism can provide in-depth insight into host response to disease and provide a platform for testing a range of treatment options. The optical transparency of zebrafish at early stages of development permits easy assessment of the effects of treatments, occurrence of tumors and other abnormal growth, disease progression, and immune response, to name only a few. These characteristics make it ideal for ...


Enhancing Co-Water Mass Transfer By Functionalized Mcm41 Nanoparticles, Haiyang Zhu, Brent H. Shanks, Theodore J. Heindel Jun 2017

Enhancing Co-Water Mass Transfer By Functionalized Mcm41 Nanoparticles, Haiyang Zhu, Brent H. Shanks, Theodore J. Heindel

Theodore J. Heindel

Organic groups are grafted to ∼250-nm-diameter MCM41 nanoparticles with a spherical morphology to enhance the CO-water volumetric mass-transfer coefficient (kLa) for synthesis gas fermentation. The results indicate that (i) ∼250-nm MCM41 nanoparticles show a higher kLa value than large silica particles (1.4 and 7 μm), (ii) surface hydroxyl groups on MCM41 nanoparticles play an important role in mass-transfer enhancement, (iii) organic groups grafted to MCM41 modify the mass-transfer enhancement, and (iv) mercaptan groups grafted to MCM41 show the most mass-transfer enhancement of ∼1.9 times that of no nanoparticle addition. The CO-water mass-transfer enhancement depends on the interaction between ...


Effect Of Electrolytes On Co-Water Mass Transfer, Haiyang Zhu, Brent H. Shanks, Theodore J. Heindel Jun 2017

Effect Of Electrolytes On Co-Water Mass Transfer, Haiyang Zhu, Brent H. Shanks, Theodore J. Heindel

Theodore J. Heindel

The influence of various electrolytes such as sulfate, nitrate, and chloride on CO-water mass transfer was investigated in this study. The results indicate that the enhancement in the CO-water volumetric masstransfer coefficient ranged from 1.5 to 4.7 times that of a baseline system without electrolytes, depending on electrolyte type and concentration. For those electrolytes with the same anions, copper-containing electrolytes provided stronger enhancement, whereas for those electrolytes with the same cations, sulfate-containing electrolytes showed stronger enhancement. By measuring both the CO-water volumetric mass-transfer coefficient (kLa) and the mass-transfer coefficient (kL), it was found that the electrolytes inhibit gas ...


Flow Cytometry Is A Promising And Rapid Method For Differentiating Between Freely Suspended Escherichia Coli And E. Coli Attached To Clay Particles, Xiao Liang, Michelle L. Soupir, Shawn M. Rigby, Laura R. Jarboe, Wei Zhang Jun 2017

Flow Cytometry Is A Promising And Rapid Method For Differentiating Between Freely Suspended Escherichia Coli And E. Coli Attached To Clay Particles, Xiao Liang, Michelle L. Soupir, Shawn M. Rigby, Laura R. Jarboe, Wei Zhang

Laura R. Jarboe

Aim: A standard procedure does not exist to distinguish between attached and unattached micro-organisms. In this study, we compared two methods to quantify between Escherichia coli attached to clay particles and E. coli freely suspended in solution: flow cytometry (attachment assay and viability assay) and settling (or centrifugation followed by settling). Methods and Results: Methods were tested using three environmental strains collected from swine facilities (A, B and C) and one purchased modified pathogenic strain (ATCC 43888); four clay particles: Hectorite, Kaolinite, Ca-Montmorillonite, Montmorillonite K-10; and a range of surface area ratios (particle surface area to E. coli surface area ...


Growth Condition Optimization For Docosahexaenoic Acid (Dha) Production By Moritella Marina Mp-1, Kumar Babu Kautharapu, John A. Rathmacher, Laura R. Jarboe Jun 2017

Growth Condition Optimization For Docosahexaenoic Acid (Dha) Production By Moritella Marina Mp-1, Kumar Babu Kautharapu, John A. Rathmacher, Laura R. Jarboe

Laura R. Jarboe

The marine organism Moritella marina MP-1 produces the polyunsaturated fatty acid docosahexaenoic acid (DHA). While the basic metabolic pathway for DHA production in this organism has been identified, the impact of growth conditions on DHA production is largely unknown. This study examines the effect of supplemental carbon, nitrogen and salts, growth temperature and media composition and pH on DHA and biomass production and the fatty acid profile. The addition of supplemental nitrogen significantly increased the overall DHA titer via an increase in biomass production. Supplemental glucose or glycerol increased biomass production, but decreased the amount of DHA per biomass, resulting ...


Electron Microscopic Observations Of Interfacial Voids In Aluminum Created By Alkaline Dissolution, Saikat Adhikari, L. Scott Chumbley, H. Chen, Y. C. Jean, A. C. Geiculescu, Kurt R. Hebert Jun 2017

Electron Microscopic Observations Of Interfacial Voids In Aluminum Created By Alkaline Dissolution, Saikat Adhikari, L. Scott Chumbley, H. Chen, Y. C. Jean, A. C. Geiculescu, Kurt R. Hebert

Kurt R. Hebert

We report evidence from electron microscopy and positron annihilation spectroscopy (PAS) for the formation by alkaline dissolution of nm-scale voids in aluminum near the metal-oxide interface. Imaging was carried out using transmission electron microscopy (TEM), scanning transmission electron microscopy (STEM) and field emission scanning electron microscopy (FESEM). EM images supported the PAS finding that voids were found within tens of nm of the interface, and revealed that the void number density increased by at least 10 times due to dissolution. From TEM, void number densities were on the order of 108 cm-2. From TEM and SEM, voids appeared circular in ...


Model For The Steady-State Growth Of Porous Anodic Alumina Films, Jerrod E. Houser, Kurt R. Hebert Jun 2017

Model For The Steady-State Growth Of Porous Anodic Alumina Films, Jerrod E. Houser, Kurt R. Hebert

Kurt R. Hebert

Simulations were developed for the distributions of electrical potential and incorporated anions in porous anodic alumina (PAA) films during steady-state growth. Predictions of a model for the potential distribution based on Laplace's equation were compared to those of the current continuity equation in conjunction with high-field conduction. It was found that Laplace's equation, which has been used previously in PAA models, resulted in strong violations of charge conservation, when the current density was evaluated using the high field conduction equation. Interface motion predicted by the current continuity equation was nearly uniform except near convex ridges on the metal-film ...


Tin Dioxide-Carbon Heterostructures Applied To Gas Sensing: Structure-Dependent Properties And General Sensing Mechanism, Catherine Marichy, Patricia A. Russo, Mariangela Latino, Jean-Philippe Tessonnier, Marc-Georg Wilinger, Nicola Donato, Giovanni Neri, Nicola Pinna Jun 2017

Tin Dioxide-Carbon Heterostructures Applied To Gas Sensing: Structure-Dependent Properties And General Sensing Mechanism, Catherine Marichy, Patricia A. Russo, Mariangela Latino, Jean-Philippe Tessonnier, Marc-Georg Wilinger, Nicola Donato, Giovanni Neri, Nicola Pinna

Jean-Philippe Tessonnier

Carbon materials such as carbon nanotubes (CNTs), graphene, and reduced graphene oxide (RGO) exhibit unique electrical properties, which are also influenced by the surrounding atmosphere. They are therefore promising sensing materials. Despite the existence of studies reporting the gas-sensing properties of metal oxide (MOx) coated nanostructured carbon, an incomplete understanding of their sensing mechanism remains. Here we report a systematic study on the preparation, characterization, and sensing properties of CNT and RGO composites with SnO2 coating. Atomic layer deposition (ALD) was applied to the conformal coating of the inner and outer walls of CNTs with thin films of SnO2 of ...


Mixed-Surface, Lipid-Tethered Quantum Dots For Targeting Cells And Tissues, Yanjie Zhang, Amanda Haage, Elizabeth M. Whitley, Ian C. Schneider, Aaron R. Clapp Jun 2017

Mixed-Surface, Lipid-Tethered Quantum Dots For Targeting Cells And Tissues, Yanjie Zhang, Amanda Haage, Elizabeth M. Whitley, Ian C. Schneider, Aaron R. Clapp

Ian C. Schneider

Quantum dots (QDs), with their variable luminescent properties, are rapidly transcending traditional labeling techniques in biological imaging and hold vast potential for biosensing applications. An obstacle in any biosensor development is targeted specificity. Here we report a facile procedure for creating QDs targeted to the cell membrane with the goal of cell-surface protease biosensing. This procedure generates water-soluble QDs with variable coverage of lipid functional groups. The resulting hydrophobicity is quantitatively controlled by the molar ratio of lipids per QD. Appropriate tuning of the hydrophobicity ensures solubility in common aqueous cell culture media and while providing affinity to the lipid ...


Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider Jun 2017

Epitaxially Grown Collagen Fibrils Reveal Diversity In Contact Guidance Behavior Among Cancer Cells, Juan Wang, Joseph W. Petefish, Andrew C. Hillier, Ian C. Schneider

Ian C. Schneider

Invasion of cancer cells into the surrounding tissue is an important step during cancer progression and is driven by cell migration. Cell migration can be random, but often it is directed by various cues such as aligned fibers composed of extracellular matrix (ECM), a process called contact guidance. During contact guidance, aligned fibers bias migration along the long axis of the fibers. These aligned fibers of ECM are commonly composed of type I collagen, an abundant structural protein around tumors. In this paper, we epitaxially grew several different patterns of organized type I collagen on mica and compared the morphology ...


Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider Jun 2017

Cellular Contractility And Extracellular Matrix Stiffness Regulate Matrix Metalloproteinase Activity In Pancreatic Cancer Cells, Amanda Haage, Ian C. Schneider

Ian C. Schneider

The pathogenesis of cancer is often driven by local invasion and metastasis. Recently, mechanical properties of the tumor microenvironment have been identified as potent regulators of invasion and metastasis, while matrix metalloproteinases (MMPs) are classically known as significant enhancers of cancer cell migration and invasion. Here we have been able to sensitively measure MMP activity changes in response to specific extracellular matrix (ECM) environments and cell contractility states. A pancreatic cancer cell line, Panc-1 cells, up-regulate MMP activities between 3- and 10- fold with increased cell contractility. Conversely, they down-regulate MMP activities when contractility is blocked to levels seen with ...


Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider Jun 2017

Collagen Attachment To The Substrate Controls Cell Clustering Through Migration, Yue Hou, Laura L. Rodriguez, Juan Wang, Ian C. Schneider

Ian C. Schneider

Cell clustering and scattering play important roles in cancer progression and tissue engineering. While the extracellular matrix (ECM) is known to control cell clustering, much of the quantitative work has focused on the analysis of clustering between cells with strong cell-cell junctions. Much less is known about how the ECM regulates cells with weak cell-cell contact. Clustering characteristics were quantified in rat adenocarcinoma cells, which form clusters on physically adsorbed collagen substrates, but not on covalently attached collagen substrates. Covalently attaching collagen inhibited desorption of collagen from the surface. While changes in proliferation rate could not explain differences seen in ...


The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider Jun 2017

The Number Of Lines A Cell Contacts And Cell Contractility Drive The Efficiency Of Contact Guidance, Nicholas R. Romsey, Yue Hou, Laura L. Rodriguez, Ian C. Schneider

Ian C. Schneider

Cell migration is an important biological function that impacts many physiological and pathological processes. Often migration is directed along various densities of aligned fibers of collagen, a process called contact guidance. However, cells adhere to other components in the extracellular matrix, possibly affecting migrational behavior. Additionally, changes in intracellular contractility are well known to affect random migration, but its effect on contact guidance is less known. This study examines differences in directed migration in response to variations in the spacing of collagen, non-specific background adhesion strength and myosin II-mediated contractility. Collagen was microcontact printed onto glass substrates and timelapse live-cell ...


H Emagglutinin-Based Polyanhydride Nanovaccines Against H5n1 Influenza Elicit Protective Virus Neutralizing Titers And Cell-Mediated Immunity, Kathleen Alaine Ross, Hyelee Park Loyd, Wuwei Wu, Lucas Mark Huntimer, Shaheen Ahmed, Anthony Sambol, Scott Broderick, Zachary Flickinger, Krishna Rajan, Tatiana Bronich, Surya K. Mallapragada, Michael J. Wannemuehler, Susan Long Carpenter, Balaji Narasimhan Jun 2017

H Emagglutinin-Based Polyanhydride Nanovaccines Against H5n1 Influenza Elicit Protective Virus Neutralizing Titers And Cell-Mediated Immunity, Kathleen Alaine Ross, Hyelee Park Loyd, Wuwei Wu, Lucas Mark Huntimer, Shaheen Ahmed, Anthony Sambol, Scott Broderick, Zachary Flickinger, Krishna Rajan, Tatiana Bronich, Surya K. Mallapragada, Michael J. Wannemuehler, Susan Long Carpenter, Balaji Narasimhan

Balaji Narasimhan

H5N1 avian influenza is a significant global concern with the potential to become the next pandemic threat. Recombinant subunit vaccines are an attractive alternative for pandemic vaccines compared to traditional vaccine technologies. In particular, polyanhydride nanoparticles encapsulating subunit proteins have been shown to enhance humoral and cell-mediated immunity and provide protection upon lethal challenge. In this work, a recombinant H5 hemagglutinin trimer (H53) was produced and encapsulated into polyanhydride nanoparticles. The studies performed indicated that the recombinant H53 antigen was a robust immunogen. Immunizing mice with H53 encapsulated into polyanhydride nanoparticles induced high neutralizing antibody titers and enhanced CD4+ T ...