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Articles 1 - 30 of 52

Full-Text Articles in Biomedical Engineering and Bioengineering

Optical Vortex And Poincaré Analysis For Biophysical Dynamics, Anindya Majumdar Jan 2019

Optical Vortex And Poincaré Analysis For Biophysical Dynamics, Anindya Majumdar

Dissertations, Master's Theses and Master's Reports

Coherent light - such as that from a laser - on interaction with biological tissues, undergoes scattering. This scattered light undergoes interference and the resultant field has randomly added phases and amplitudes. This random interference pattern is known as speckles, and has been the subject of multiple applications, including imaging techniques. These speckle fields inherently contain optical vortices, or phase singularities. These are locations where the intensity (or amplitude) of the interference pattern is zero, and the phase is undefined.

In the research presented in this dissertation, dynamic speckle patterns were obtained through computer simulations as well as laboratory setups involving scattering ...


In Vivo Brainstem Imaging In Alzheimer’S Disease: Potential For Biomarker Development, David J. Braun, Linda J. Van Eldik Sep 2018

In Vivo Brainstem Imaging In Alzheimer’S Disease: Potential For Biomarker Development, David J. Braun, Linda J. Van Eldik

Neuroscience Faculty Publications

The dearth of effective treatments for Alzheimer’s disease (AD) is one of the largest public health issues worldwide, costing hundreds of billions of dollars per year. From a therapeutic standpoint, research efforts to date have met with strikingly little clinical success. One major issue is that trials begin after substantial pathological change has occurred, and it is increasingly clear that the most effective treatment regimens will need to be administered earlier in the disease process. In order to identify individuals within the long preclinical phase of AD who are likely to progress to dementia, improvements are required in biomarker ...


Fluorescent Nanodiamonds: Past, Present, And Future, Masfer H. Alkahtani, Fahad Alghannam, Linkun Jiang, Abdulrahman Almethen, Arfaan A. Rampersaud, Robert Brick, Carmen L. Gomes, Marlan O. Scully, Philip R. Hemmer Jul 2018

Fluorescent Nanodiamonds: Past, Present, And Future, Masfer H. Alkahtani, Fahad Alghannam, Linkun Jiang, Abdulrahman Almethen, Arfaan A. Rampersaud, Robert Brick, Carmen L. Gomes, Marlan O. Scully, Philip R. Hemmer

Mechanical Engineering Publications

Multi-color fluorescent nanodiamonds (FNDs) containing a variety of color centers are promising fluorescent markers for biomedical applications. Compared to colloidal quantum dots and organic dyes, FNDs have the advantage of lower toxicity, exceptional chemical stability, and better photostability. They can be surface functionalized by techniques similar to those used for other nanoparticles. They exhibit a variety of emission wavelengths from visible to near infrared, with narrow or broad bandwidths depending on their color centers. In addition, some color centers can detect changes in magnetic fields, electric fields, and temperature. In this article review, we will discuss the current trends in ...


Fluorescence Guided Tumor Imaging: Foundations For Translational Applications, Jessica P. Miller May 2018

Fluorescence Guided Tumor Imaging: Foundations For Translational Applications, Jessica P. Miller

Engineering and Applied Science Theses & Dissertations

Optical imaging for medical applications is a growing field, and it has the potential to improve medical outcomes through its increased sensitivity and specificity, lower cost, and small instrumentation footprint as compared to other imaging modalities. The method holds great promise, ranging from direct clinical use as a diagnostic or therapeutic tool, to pre-clinical applications for increased understanding of pathology. Additionally, optical imaging uses non-ionizing radiation which is safe for patients, so it can be used for repeated imaging procedures to monitor therapy, guide treatment, and provide real-time feedback. The versatile features of fluorescence-based optical imaging make it suited for ...


Development Of Low Frequency Electron Paramagnetic Resonance Methods And Instrumentation For Biological Applications, Laura A. Buchanan Jan 2018

Development Of Low Frequency Electron Paramagnetic Resonance Methods And Instrumentation For Biological Applications, Laura A. Buchanan

Electronic Theses and Dissertations

EPR is a powerful biophysical tool that can be used to measure tumor physiology. With the addition of magnetic field gradients, the spectral properties of paramagnetic species can be mapped. To facilitate EPR imaging, methods and instrumentation at frequencies between 250 MHz and 1 GHz were developed.

At low spin concentrations, the rapid scan background signal is often many times larger than the EPR signal of interest. To help remove the background contribution, a data acquisition procedure that takes advantage of a cross-loop resonator and bipolar power supplies was developed at 250 MHz. In this procedure, two scans are collected ...


Experimental And Model-Based Terahertz Imaging And Spectroscopy For Mice, Human, And Phantom Breast Cancer Tissues, Tyler Bowman Jan 2018

Experimental And Model-Based Terahertz Imaging And Spectroscopy For Mice, Human, And Phantom Breast Cancer Tissues, Tyler Bowman

Theses and Dissertations

The goal of this work is to investigate terahertz technology for assessing the surgical margins of breast tumors through electromagnetic modeling and terahertz experiments. The measurements were conducted using a pulsed terahertz system that provides time and frequency domain signals. Three types of breast tissues were investigated in this work. The first was formalin-fixed, paraffin-embedded tissues from human infiltrating ductal and lobular carcinomas. The second was human tumors excised within 24-hours of lumpectomy or mastectomy surgeries. The third was xenograft and transgenic mice breast cancer tumors grown in a controlled laboratory environment to achieve more data for statistical analysis.

Experimental ...


Chelator-Free Radiolabeling Of Serrs Nanoparticles For Whole-Body Pet And Intraoperative Raman Imaging, Matthew A. Wall, Travis Shaffer, Stefan Harmsen, Darjus-Felix Tschaharganeh, Chun-Hao Huang, Scott W. Lowe, Charles Michael Drain, Moritz F. Kircher Jul 2017

Chelator-Free Radiolabeling Of Serrs Nanoparticles For Whole-Body Pet And Intraoperative Raman Imaging, Matthew A. Wall, Travis Shaffer, Stefan Harmsen, Darjus-Felix Tschaharganeh, Chun-Hao Huang, Scott W. Lowe, Charles Michael Drain, Moritz F. Kircher

Publications and Research

A single contrast agent that offers whole-body non-invasive imaging along with the superior sensitivity and spatial resolution of surface-enhanced resonance Raman scattering (SERRS) imaging would allow both pre-operative mapping and intraoperative imaging and thus be highly desirable. We hypothesized that labeling our recently reported ultrabright SERRS nanoparticles with a suitable radiotracer would enable pre-operative identification of regions of interest with whole body imaging that can be rapidly corroborated with a Raman imaging device or handheld Raman scanner in order to provide high precision guidance during surgical procedures. Here we present a straightforward new method that produces radiolabeled SERRS nanoparticles for ...


Radiographic Assessment Of Lung Anatomy, Physiology, And Disease In A Porcine Model Of Cystic Fibrosis And People With Cystic Fibrosis, Ryan J. Adam May 2017

Radiographic Assessment Of Lung Anatomy, Physiology, And Disease In A Porcine Model Of Cystic Fibrosis And People With Cystic Fibrosis, Ryan J. Adam

Theses and Dissertations

Despite affecting many organ systems, the leading cause of morbidity and mortality in the cystic fibrosis (CF) population is lung disease. For the current studies we investigated elements of CF lung disease in a porcine model of CF and in people with CF. Our primary analysis tool was chest computed tomography (CT).

To investigate early CF lung disease we examined three week old CF and non-CF pigs. We found three week old CF pigs to have large, irregular tracheal smooth muscle bundles, airways of reduced size, airways of irregular shape, and airways of abnormal distensibility.

Three week old CF pig ...


Multimodal Evaluation Of Local And Whole-Joint Cartilage Changes In An In Vivo Animal Model, David James Heckelsmiller May 2017

Multimodal Evaluation Of Local And Whole-Joint Cartilage Changes In An In Vivo Animal Model, David James Heckelsmiller

Theses and Dissertations

Osteoarthritis is a chronic, deleterious disease of the joints. It currently affects nearly 25 million Americans. Clinically, osteoarthritis presents as joint pain and verified by radiographic evidence of joint space narrowing. Unfortunately, symptomatic osteoarthritis describes the later stages of disease, at which point irreversible cartilage and bone damage has occurred. Cross-sectional imaging modalities offer the promise of visualizing early features of disease, enabling the development and evaluation of interventions to forestall or prevent degenerative change. Modalities of clinical interest include magnetic resonance imaging (MRI) and multi-detector computed tomography (MDCT).

The following work describes the efficacy of MRI-derived measures for the ...


Developing An Imaging Biomarker To Detect Aberrant Brain Connectivity In Individual Patients, Esther Cox Apr 2017

Developing An Imaging Biomarker To Detect Aberrant Brain Connectivity In Individual Patients, Esther Cox

Master's Theses (2009 -)

Resting state functional MRI (rsfMRI) has been proven to be a valuable tool in clinical applications such as pre-surgical mapping, but there is not yet a functional and usable algorithm that can be used by physicians in a clinical setting to evaluate an individual patient for diseases and aberrant brain connectivity. If a physician wants to evaluate a patient in this way, the rsfMRI data must be looked at “by hand,” i.e. the physician must manually evaluate the data and identify the functional ICN’s and whether they are normal or aberrant. An algorithm that would automate this process ...


A Structural And Functional Analysis Of Human Brain Mri With Attention Deficit Hyperactivity Disorder, Arjun A. Watane Jan 2017

A Structural And Functional Analysis Of Human Brain Mri With Attention Deficit Hyperactivity Disorder, Arjun A. Watane

Honors Undergraduate Theses

Attention Deficit Hyperactivity Disorder (ADHD) affects 5-10% of children worldwide. Its effects are mainly behavioral, manifesting in symptoms such as inattention, hyperactivity, and impulsivity. If not monitored and treated, ADHD may adversely affect a child's health, education, and social life. Furthermore, the neurological disorder is currently diagnosed through interviews and opinions of teachers, parents, and physicians. Because this is a subjective method of identifying ADHD, it is easily prone to error and misdiagnosis. Therefore, there is a clear need to develop an objective diagnostic method for ADHD.

The focus of this study is to explore the use of machine ...


Photoreceptor Inner Segment Morphology In Best Vitelliform Macular Dystrophy, Drew Scoles, Yusufu N. Sulai, Robert F. Cooper, Brian P. Higgins, Ryan D. Johnson, Joseph Carroll, Alfredo Dubra, Kimberly E. Stepien Oct 2016

Photoreceptor Inner Segment Morphology In Best Vitelliform Macular Dystrophy, Drew Scoles, Yusufu N. Sulai, Robert F. Cooper, Brian P. Higgins, Ryan D. Johnson, Joseph Carroll, Alfredo Dubra, Kimberly E. Stepien

Biomedical Engineering Faculty Research and Publications

PURPOSE

To characterize outer retina structure in best vitelliform macular dystrophy (BVMD) and to determine the effect of macular lesions on overlying and adjacent photoreceptors.

METHODS

Five individuals with BVMD were followed prospectively with spectral domain optical coherence tomography and confocal and nonconfocal split-detector adaptive optics scanning light ophthalmoscopy (AOSLO). The AOSLO cone photoreceptor mosaic images were obtained within and around retinal lesions. Cone density was measured inside and outside lesions. In 2 subjects, densities were compared with published measurements acquired ∼2.5 years before. One subject was imaged 3 times over a 5-month period.

RESULTS

The AOSLO imaging demonstrated ...


Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj Aug 2016

Nanobubbles Provide Theranostic Relief To Cancer Hypoxia, Christopher M. Long, Pushpak N. Bhandari, Joseph Irudayaraj

The Summer Undergraduate Research Fellowship (SURF) Symposium

Hypoxia is a common motif among tumors, contributing to metastasis, angiogenesis, cellular epigenetic abnormality, and resistance to cancer therapy. Hypoxia also plays a pivotal role in oncological studies, where it can be used as a principal target for new anti-cancer therapeutic methods. Oxygen nanobubbles were designed in an effort to target the hypoxic tumor regions, thus interrupting the hypoxia-inducible factor-1α (HIF-1α) regulatory pathway and inhibiting tumor progression. At less than 100nm, oxygen nanobubbles act as a vehicle for site-specific oxygen delivery, while also serving as an ultrasound contrast agent for advanced imaging purposes. Through in vitro and in vivo studies ...


Machine Learning Methods For Medical And Biological Image Computing, Rongjian Li Jul 2016

Machine Learning Methods For Medical And Biological Image Computing, Rongjian Li

Computer Science Theses & Dissertations

Medical and biological imaging technologies provide valuable visualization information of structure and function for an organ from the level of individual molecules to the whole object. Brain is the most complex organ in body, and it increasingly attracts intense research attentions with the rapid development of medical and bio-logical imaging technologies. A massive amount of high-dimensional brain imaging data being generated makes the design of computational methods for efficient analysis on those images highly demanded. The current study of computational methods using hand-crafted features does not scale with the increasing number of brain images, hindering the pace of scientific discoveries ...


Proof That Can Travel - Documented Clonality Report For Regulatory Submission, Ian Taylor, Paul Miller May 2016

Proof That Can Travel - Documented Clonality Report For Regulatory Submission, Ian Taylor, Paul Miller

Cell Culture Engineering XV

Clonality is a key element of cell line development and is an important component of a regulatory submission. Indeed for BLA, the clonality of MCB is mandatory. Historically, the regulator has insisted upon 2 rounds of cloning for developing a cell line and assurance of monoclonality based on statistical outgrowth measurements. Recent improvements in high resolution whole well imaging of cells in microplates, enables the creation of indisputable image-based evidence for the growth of a colony from a single cell. This evidence eliminates a round of sub-cloning and results in several months of cell line development time being saved. However ...


Increasing 18f-Fdg Pet/Ct Capabilities In Radiotherapy For Lung And Esophageal Cancer Via Image Feature Analysis, Jasmine Alexandria Oliver Mar 2016

Increasing 18f-Fdg Pet/Ct Capabilities In Radiotherapy For Lung And Esophageal Cancer Via Image Feature Analysis, Jasmine Alexandria Oliver

Graduate Theses and Dissertations

Positron Emission Tomography (PET) is an imaging modality that has become increasingly beneficial in Radiotherapy by improving treatment planning (1). PET reveals tumor volumes that are not well visualized on computed tomography CT or MRI, recognizes metastatic disease, and assesses radiotherapy treatment (1). It also reveals areas of the tumor that are more radiosensitive allowing for dose painting - a non-homogenous dose treatment across the tumor (1). However, PET is not without limitations. The quantitative unit of PET images, the Standardized Uptake Value (SUV), is affected by many factors such as reconstruction algorithm, patient weight, and tracer uptake time (2). In ...


Europium(Ii)-Containing Complexes For Responsive Magnetic Resonance Imaging, Levi Alexander Ekanger Jan 2016

Europium(Ii)-Containing Complexes For Responsive Magnetic Resonance Imaging, Levi Alexander Ekanger

Wayne State University Dissertations

The research projects described in this dissertation are focused on studying the oxidation of EuII-containing complexes within the context of responsive MRI. Prior to this research, EuII had not been explored within the context of oxidation-responsive MRI nor had the use of this ion been reported in vivo. The results of these studies enable predictions about the oxidation-responsive behavior of EuII-containing complexes in vitro and in vivo.

The EuII-containing cryptate 1.33 was used to evaluate EuII-based positive contrast enhancement after intravenous, intraperitoneal, and subcutaneous injections. The transitory behavior of the positive contrast enhancement correlated with reported levels of oxygenation ...


Recent Advances In Optical Spectroscopic And Imaging Methods For Medicine And Biology, Yu Shang, Zhiyu Qian, Rickson C. Mesquita, Mathieu Dehaes Jan 2016

Recent Advances In Optical Spectroscopic And Imaging Methods For Medicine And Biology, Yu Shang, Zhiyu Qian, Rickson C. Mesquita, Mathieu Dehaes

Biomedical Engineering Faculty Publications

No abstract provided.


Ultrasound-Guided Nanobubbles For Targeted Drug Delivery, Pei Yang, Pushpak Bhandari, Joseph Irudayaraj Aug 2015

Ultrasound-Guided Nanobubbles For Targeted Drug Delivery, Pei Yang, Pushpak Bhandari, Joseph Irudayaraj

The Summer Undergraduate Research Fellowship (SURF) Symposium

In a large number of biological and environmental applications, ultrasound (US)-powered micro- and nano-motors have attracted considerable attention. However, their applications in biological settings have been limited due to the incompatibility of metallic motors and the lack of precision guiding. Here, we demonstrate that cellulosic polymer nanobubbles (200-800nm) can be propelled, aligned, accelerated, and assembled in solution using Doppler ultrasound beam (DUB) and simultaneously imaged using low-frequency ultrasound. Results show that by utilizing Doppler ultrasound beam (DUB), nanobubbles accumulation at a pre-determined site can be enhanced. Moreover, bubbles’ trajectory and velocity can be also be manipulated. Related parameters associated ...


3d Thoracoscopic Ultrasound Volume Measurement Validation In An Ex Vivo And In Vivo Porcine Model Of Lung Tumours, V. Hornblower, E. Yu, A. Fenster, J. Battista, R. Malthaner Jul 2015

3d Thoracoscopic Ultrasound Volume Measurement Validation In An Ex Vivo And In Vivo Porcine Model Of Lung Tumours, V. Hornblower, E. Yu, A. Fenster, J. Battista, R. Malthaner

Richard A. Malthaner

The purpose of this study was to validate the accuracy and reliability of volume measurements obtained using three-dimensional (3D) thoracoscopic ultrasound (US) imaging. Artificial "tumours" were created by injecting a liquid agar mixture into spherical moulds of known volume. Once solidified, the "tumours" were implanted into the lung tissue in both a porcine lung sample ex vivo and a surgical porcine model in vivo. 3D US images were created by mechanically rotating the thoracoscopic ultrasound probe about its long axis while the transducer was maintained in close contact with the tissue. Volume measurements were made by one observer using the ...


3d Thoracoscopic Ultrasound Volume Measurement Validation In An Ex Vivo And In Vivo Porcine Model Of Lung Tumours, V. Hornblower, E. Yu, A. Fenster, J. Battista, R. Malthaner Jul 2015

3d Thoracoscopic Ultrasound Volume Measurement Validation In An Ex Vivo And In Vivo Porcine Model Of Lung Tumours, V. Hornblower, E. Yu, A. Fenster, J. Battista, R. Malthaner

Richard A. Malthaner

The purpose of this study was to validate the accuracy and reliability of volume measurements obtained using three-dimensional (3D) thoracoscopic ultrasound (US) imaging. Artificial "tumours" were created by injecting a liquid agar mixture into spherical moulds of known volume. Once solidified, the "tumours" were implanted into the lung tissue in both a porcine lung sample ex vivo and a surgical porcine model in vivo. 3D US images were created by mechanically rotating the thoracoscopic ultrasound probe about its long axis while the transducer was maintained in close contact with the tissue. Volume measurements were made by one observer using the ...


Characterization Of Low Density Intracranial Lesions Using Dual-Energy Computed Tomography, Jessica L. Nute May 2015

Characterization Of Low Density Intracranial Lesions Using Dual-Energy Computed Tomography, Jessica L. Nute

UT GSBS Dissertations and Theses (Open Access)

Calcific and hemorrhagic foci of susceptibility are frequently encountered on routine brain MR studies. Both etiologies cause variations in local magnetic field strength, leading to dark regions on the MR images that cannot be classified. Single-energy CT (SECT) can be used to identify lesions with attenuation over 100 HU as calcific, however lesions with lower attenuation cannot be reliably identified. While calcific lesions are unlikely to cause harm, hemorrhagic lesions carry a risk of subsequent intracranial bleeding; as such, identification of hemorrhage is vital in preventing the inappropriate use of anticoagulant medications in patients with hemorrhagic lesions.

Given there currently ...


Molecular Sensing And Imaging Of Human Disease Cells And Their Responses To Biochemical Stimuli, Lifu Xiao May 2015

Molecular Sensing And Imaging Of Human Disease Cells And Their Responses To Biochemical Stimuli, Lifu Xiao

All Graduate Theses and Dissertations

The overall goal of this dissertation is to develop noninvasive imaging techniques that allow us not only to detect diseased cells but also to study the molecular mechanisms underlying these diseases.

Atomic force microscopy and Raman spectroscopy are applied to measure cellular mechanical properties (e.g. Young’s Modulus, adhesion force) and biochemical composition of living cancerous vs. healthy (A549 vs. SAEC) human lung epithelial cells. These biomechanical and biochemical properties can be utilized to differentiate between cancerous A549 and healthy SAEC human lung epithelial cells. Furthermore, different cellular responses to anticancer drug doxorubicin (DOX) treatment are also observed. Using ...


A Novel Fmri Paradigm Suggests That Pedaling-Related Brain Activation Is Altered After Stroke, Nutta-On Promjunyakul, Brian D. Schmit, Sheila M. Schindler-Ivens Jan 2015

A Novel Fmri Paradigm Suggests That Pedaling-Related Brain Activation Is Altered After Stroke, Nutta-On Promjunyakul, Brian D. Schmit, Sheila M. Schindler-Ivens

Physical Therapy Faculty Research and Publications

The purpose of this study was to examine the feasibility of using functional magnetic resonance imaging (fMRI) to measure pedaling-related brain activation in individuals with stroke and age-matched controls. We also sought to identify stroke-related changes in brain activation associated with pedaling. Fourteen stroke and 12 control subjects were asked to pedal a custom, MRI-compatible device during fMRI. Subjects also performed lower limb tapping to localize brain regions involved in lower limb movement. All stroke and control subjects were able to pedal while positioned for fMRI. Two control subjects were withdrawn due to claustrophobia, and one control data set was ...


Three Dimensional Quantification Of Angiotensin Ii-Induced Murine Abdominal Aortic Aneurysms Using High Frequency Ultrasound, Amelia R. Adelsperger, Alexa A. Yrineo, Hilary D. Schroeder, Ashley Nicole Blaize, Katherine E. Wilson, Evan H. Phillips, Frederick W. Damen, Craig J. Goergen Aug 2014

Three Dimensional Quantification Of Angiotensin Ii-Induced Murine Abdominal Aortic Aneurysms Using High Frequency Ultrasound, Amelia R. Adelsperger, Alexa A. Yrineo, Hilary D. Schroeder, Ashley Nicole Blaize, Katherine E. Wilson, Evan H. Phillips, Frederick W. Damen, Craig J. Goergen

The Summer Undergraduate Research Fellowship (SURF) Symposium

Abdominal aortic aneurysms (AAAs), a localized dilation of the vessel wall of 50% or more above normal, claims approximately 14,000 U.S. lives yearly due to aortic rupture. This commonly asymptomatic disease can only be treated by endovascular stent grafts or invasive surgery, usually after the AAA diameter reaches 5 cm. Because these treatment methods carry serious risk, stem cell therapy is being explored in order to provide a low risk option for managing smaller AAAs. To determine if stem cell therapy, once administered, could stabilize or reduce AAA growth, baseline 3D ultrasound measurements in a control group were ...


Integration Of Instrumentation And Processing Software Of A Laser Speckle Contrast Imaging System, Jacob James Carrick Jan 2014

Integration Of Instrumentation And Processing Software Of A Laser Speckle Contrast Imaging System, Jacob James Carrick

Dissertations, Master's Theses and Master's Reports - Open

Laser speckle contrast imaging (LSCI) has the potential to be a powerful tool in medicine, but more research in the field is required so it can be used properly. To help in the progression of Michigan Tech's research in the field, a graphical user interface (GUI) was designed in Matlab to control the instrumentation of the experiments as well as process the raw speckle images into contrast images while they are being acquired. The design of the system was successful and is currently being used by Michigan Tech's Biomedical Engineering department. This thesis describes the development of the ...


Image Registration Of Ex-Vivo Mri To Sparsely Sectioned Histology Of Hippocampal And Neocortical Temporal Lobe Specimens., Maged Goubran, Cathie Crukley, Sandrine De Ribaupierre, Terence M Peters, Ali R Khan Dec 2013

Image Registration Of Ex-Vivo Mri To Sparsely Sectioned Histology Of Hippocampal And Neocortical Temporal Lobe Specimens., Maged Goubran, Cathie Crukley, Sandrine De Ribaupierre, Terence M Peters, Ali R Khan

Robarts Imaging Publications

Intractable or drug-resistant epilepsy occurs in up to 30% of epilepsy patients, with many of these patients undergoing surgical excision of the affected brain region to achieve seizure control. Recent magnetic resonance imaging (MRI) sequences and analysis techniques have the potential to detect abnormalities not identified with diagnostic MRI protocols. Prospective studies involving pre-operative imaging and collection of surgically-resected tissue provide a unique opportunity for verification and tuning of these image analysis techniques, since direct comparison can be made against histopathology, and can lead to better prediction of surgical outcomes and potentially less invasive procedures. To carry out MRI and ...


Automated Point-Of-Care Image Processing Methodology For The Diagnosis Of Malaria, Michael B. Jorgensen Jan 2013

Automated Point-Of-Care Image Processing Methodology For The Diagnosis Of Malaria, Michael B. Jorgensen

Master's Theses

Malaria has profoundly influenced human history for over four thousand years and despite numerous attempts at eradication, the prevention, diagnosis, and treatment of malaria have been largely ineffective. More than five hundred million people are affected by malaria every year resulting in over one million deaths. Drug resistance development by the parasite has diminished the effectiveness of numerous treatment options due, in part, to overtreatment of negative patients based on insufficient clinical algorithms and diagnostic methods. The goal of this research was to develop an image analysis algorithm to diagnose malaria with a high degree of sensitivity and specificity in ...


Correct Spectral Conversion Between Surface‐Enhanced Raman And Plasmon Resonance Scattering From Nanoparticle Dimers For Single‐Molecule Detection, Kyuwan Lee Dec 2012

Correct Spectral Conversion Between Surface‐Enhanced Raman And Plasmon Resonance Scattering From Nanoparticle Dimers For Single‐Molecule Detection, Kyuwan Lee

Kyuwan Lee

Simultaneous measurement of surface-enhanced Raman scattering (SERS) and localized surface plasmon resonance (LSPR) in nanoparticle dimers presents outstanding opportunities in molecular identification and in the elucidation of physical properties, such as the size, distance, and deformation of target species. SERS–LSPR instrumentation exists and has been used under limited conditions, but the extraction of SERS and LSPR readouts from a single measurement is still a challenge. Herein, the extraction of LSPR spectra from SERS signals is reported and a tool for measuring the interparticle distance from Raman enhancement data by the standardization of the SERS signal is proposed. The SERS ...


Quantitative, Spectrally-Resolved Intraoperative Fluorescence Imaging, Pablo A. Valdés, Frederic Leblond, Valerie L. Jacobs, Brian C. Wilson, Keith D. Paulsen, David W. Roberts Nov 2012

Quantitative, Spectrally-Resolved Intraoperative Fluorescence Imaging, Pablo A. Valdés, Frederic Leblond, Valerie L. Jacobs, Brian C. Wilson, Keith D. Paulsen, David W. Roberts

Open Dartmouth: Faculty Open Access Scholarship

Intraoperative visual fluorescence imaging (vFI) has emerged as a promising aid to surgical guidance, but does not fully exploit the potential of the fluorescent agents that are currently available. Here, we introduce a quantitative fluorescence imaging (qFI) approach that converts spectrally-resolved data into images of absolute fluorophore concentration pixel-by-pixel across the surgical field of view (FOV). The resulting estimates are linear, accurate, and precise relative to true values, and spectral decomposition of multiple fluorophores is also achieved. Experiments with protoporphyrin IX in a glioma rodent model demonstrate in vivo quantitative and spectrally-resolved fluorescence imaging of infiltrating tumor margins for the ...