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Biomedical Engineering and Bioengineering Commons™
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Full-Text Articles in Biomedical Engineering and Bioengineering
A Quantitative Method Of Measuring Cell-Substrate Adhesion Areas, R. G. Richards, G. Rh Owen, B. A. Rahn, I. Ap Gwynn
A Quantitative Method Of Measuring Cell-Substrate Adhesion Areas, R. G. Richards, G. Rh Owen, B. A. Rahn, I. Ap Gwynn
Cells and Materials
Variability in measurements of the 'cell adhesion strength' of fibroblasts to substrates using mechanical disruption techniques causes difficulty in determining precisely the position, in the cytoskeleton-focal adhesion-matrix -substrate interface, where failure has occurred. In the present study, a quantitative in vitro procedure for measuring the total area and percentage of fibroblast adhesion to biomaterials, using the scanning electron microscope (SEM), is described. The amount of adhesion ofL929 and Balb/c3T3 fibroblasts to discs of stainless steel, commercially pure titanium, and polyethylene terepthalate (Thermanox) was quantified. Cells were fixed, stained with heavy metals, dehydrated and embedded in resin. The resin blocks were …
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cell Adhesion To Crystal Surfaces: A Model For Initial Stages In The Attachment Of Cells To Solid Substrates, D. Hanein, B. Geiger, L. Addadi
Cells and Materials
This study addresses the mechanism of the chirally-restricted, ROD-independent adhesion of A6 epithelial cells to the {011} faces of calcium {R,R)-tartrate tetrahydrate crystals. The extensive and rapid adhesion of the cells to these surfaces, in the presence or absence of serum proteins, is distinctly different from the extracellular matrix-mediated adhesion to conventional tissue culture surfaces or to the {101} faces of the same crystals. The differences are manifested by insensitivity to ATP depletion, to disruption of microfilaments and microtubules and even to formaldehyde fixation of the cells. Furthermore, trypsin pretreatment does not affect cell attachment to the {011} faces, nor …
Quantitative Scanning Electron Microscopy (Sem) To Study The Adhesion And Spreading Of Human Endothelial Cells To Surface-Modified Poly(Carbonate Urethane)S, C. James Kirkpatrick, Tillmann Otterbach, Dirk Anderheiden, Johannes Schiefer, Horst Richter, Hartwig Hartwig Hocker, Christian Mittermayer, Albert Dekker
Quantitative Scanning Electron Microscopy (Sem) To Study The Adhesion And Spreading Of Human Endothelial Cells To Surface-Modified Poly(Carbonate Urethane)S, C. James Kirkpatrick, Tillmann Otterbach, Dirk Anderheiden, Johannes Schiefer, Horst Richter, Hartwig Hartwig Hocker, Christian Mittermayer, Albert Dekker
Cells and Materials
The polymers currently in use as vascular prostheses are, in the native state, poor substrates for endothelial cell (EC) adhesion and growth. This has a negative effect on the success of pre-seeding regimes. One attempt to improve the polymer substrate is to covalently couple reactive molecules to the surface, which can be used as anchorage points for EC or serve as spacer molecule to couple biological signal molecules such as oligopeptides. We have used a digitized image analysis system coupled to a scanning electron microscope to study the adhesion and spreading of human venous EC to unmodified poly(carbonate urethane) (uPCU), …