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Biomedical Engineering and Bioengineering Commons™
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Articles 1 - 4 of 4
Full-Text Articles in Biomedical Engineering and Bioengineering
Validation Of Energy Dispersive X-Ray Spectrometry As A Method To Standardize Backscattered Electron Images Of Bone, E. G. Vajda, R. D. Bloebaum, J. G. Skedros
Validation Of Energy Dispersive X-Ray Spectrometry As A Method To Standardize Backscattered Electron Images Of Bone, E. G. Vajda, R. D. Bloebaum, J. G. Skedros
Cells and Materials
The use of backscattered electron (BSE) imaging as a tool for the qualitative measurement of mineral content in bone has been well documented. The challenge still remains to develop BSE imaging as a tool for quantitative mineral content analysis in bone. The limiting factor has been the ability to standardize the BSE signal within and between laboratories. Energy dispersive x-ray spectrometry (EDX) has been proposed as a method to standardize the BSE operating environment. The goal of this study is to investigate the relationship between EDX-determined wt.% Ca measurements and BSE graylevels. A comparison with traditional ash content measurements will …
Investigation Of Bone And Calcium Phosphate Coatings And Crystallinity Determination Using Raman Microspectroscopy, C. J. De Grauw, J. D. De Bruijn, C. Otto, J. Greve
Investigation Of Bone And Calcium Phosphate Coatings And Crystallinity Determination Using Raman Microspectroscopy, C. J. De Grauw, J. D. De Bruijn, C. Otto, J. Greve
Cells and Materials
Conventional spontaneous Raman micro-spectroscopy was used for the investigation of bone and calcium phosphate coatings on bone-implant surfaces. Chemical and structural changes at the bone-coating interface could be monitored on a microscopic scale. It was shown that the crystallinity did not vary within the crystalline apatite and amorphous calcium phosphate coatings, while the density within each coating did vary. Different degrees of crystallinity in coatings were investigated for a series of plasma-sprayed apatite coatings. It is concluded that Raman microspectroscopy is an easy non-destructive way to obtain information about the apatite structure and the degree of crystallinity.
Gap Junctional Intercellular Communication In Bone, Henry J. Donahue, Monique A. Vander Molen, Clare E. Yellowley, Zhongyong Li
Gap Junctional Intercellular Communication In Bone, Henry J. Donahue, Monique A. Vander Molen, Clare E. Yellowley, Zhongyong Li
Cells and Materials
Gap junctional intercellular communication has been demonstrated in bone cells and may contribute to the mechanism by which osteoblasts integrate and amplify extracellular signals, both chemical (hormonal) and biophysical (electrical and mechanical). Connexin 43 (Cx43) is the predominant gap junction protein expressed by bone cells. Experiments with osteoblastic cells in which Cx43 expression was diminished by antisense transfection demonstrate that cell-to-cell coupling in osteoblastic ROS 17/2.8 cells is via gap junctions composed of Cx43. Cellular networks of these coupling deficient clones are dramatically less responsive to parathyroid hormone (PTH) suggesting that coupling contributes to hormonal responsiveness. Furthermore, PTH per se …
Perturbations Of Periosteel Bone During Healing: Effect Of Non-Weight Bearing, Helen E. Gruber, Mary E. Kirchen, John R. Sweeney, William E. Anderson, G. June Marshall
Perturbations Of Periosteel Bone During Healing: Effect Of Non-Weight Bearing, Helen E. Gruber, Mary E. Kirchen, John R. Sweeney, William E. Anderson, G. June Marshall
Cells and Materials
Weight bearing (WB) is an important factor influencing bone remodelling. The present study evaluates the effects of weight bearing and non-weight bearing (S) (achieved by tail suspension and hindlimb elevation) on the healing of a fibular osteotomy in adult male rats. After 9, 18 or 36 days under WB or S conditions, periosteum near the callus formed at the osteotomy site was compared to periosteum of the contralateral fibula (which did not receive an osteotomy) or to periosteum of fibula of control animals which did not receive an osteotomy. Data show that periosteal bone healing is sensitive to alterations in …